Electrophoretic Studies of Tissue Specific Inhibition of Esterases In Different Tissues (Intestine,Muscle,Brain) Of Fresh Water Fish Channa punctatus Exposed To Enzyme Inhibitors Paraxon, Eserine , Parachloro Mercuric Benzoate
Keywords:
Channa punctatus, electrophoretic studies, intestine, muscle, brain, tissue specific inhibition, Esterases, Paraxon, Eserine, pCMBAbstract
The current research was under taken to discover the electrophoretic studies of tissue specific inhibition of esterases in different tissues (intestine, muscle, brain) of Channa punctatus exposed to enzyme inhibitors i.e. Paraxon, Eserine (Physostigmine), Parachloro Mercuric Benzoate (pCMB). The inhibition studies of Esterases were conducted as per the standardized procedures.The results revealed that Intestine tissue E-1 are CHsp esterases which are inhibited by all the three inhibitors. E-2 are Carboxyl Esterases (CE) which are inhibited by paraoxon only. While E-3 are Ese-Esterases which are inhibited by eserene only. Muscle tissue E-1 are CHsp, E-2 are enzymes resistent to inhibitors (ER)esterases , which are not inhibited by all inhibitors, E-3 are Ese-esterases which are inhibited by eserene only. Brain tissue E-1 and E-2 are Carboxyl Esterases (CE) which are inhibited by paraoxon only, E-3 are Cholinesterases (ChE) which are inhibited by both paraoxon and eserine.
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