Divergence analysis of Phosphoglucomutase from two members of family Asteraceae by applying computational tools

Authors

  • Thakre Swapnil B Bioinformatics Laboratory, Department of Botany, Sant Gadge Baba Amravati University, Amravati. 444602, India

Keywords:

Asteraceae, CLUSTAL-W, Phylogenetic analysis, Phosphoglucomutase (PGM)

Abstract

Phylogenetic relationships of Phosphoglucomutase enzyme sequences was performed in two members i.e. Helianthus annus L. and Lactuca sativa L. of family Asteraceae by applying CLUSTAL-W programme. Ten sequences of phosphoglucomutase (PGM) from each species those are already deposited on NCBI were subjected to Multiple Sequence Alignment (MSA). Dendrogram reveals that 20 phosphoglucomutase sequence divided into two distinct clusters. The cluster-I consist of 8 phosphoglucomutase sequences from Lactuca sativa, whereas cluster-II consist of 12 phosphoglucomutase sequences belonging to Helianthus annus L. and Lactuca sativa L.

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References

1. Almeida MR and SM Almeida (2001) A floura of Maharashtra. For Blattern herbarium st. Xavier College, 3: 113-118.

2. Bayer RJ and JR Starr (1998). Tribal phylogeny of the Asteraceae based on two non-coding chloroplast sequences, the trnL intron and trnL/trnF intergenic spacer. Annals of the Missouri Botanical Garden, 85: 242–256.

3. Ben-Zvi R and M Schramm (1961) A Phosphoglucomutase specific for beta-glucose 1-phosphate. J. Biol. Chem., 236:2186-2189.

4. Bremer K (1994) Asteraceae: cladistics and classification van Nielsen Article first published online: 28 Jun 2008 DOI: 10.1111/j.1756-1051.1994.tb00632.x

5. Dangi KS, Arya V and SN Mishra (2011) CLUSTAL W Analysis of cytochrom b based genetic diversity of osteichthyes: Asian J. Exp. Biol. Sci., 2 (1): 108-113.

6. Denda T, Watanabe K, Kosuge K, Yahara T and M Ito (1999) Molecular phylogeny of Brachycome (Asteraceae). Plant Systematics and Evolution, 217: 299–311.

7. Duret L and S Abdeddaim (2000) Multiple alignment for structural, functional or phylogenetic analyses of homologous sequences. In: Higgins D. and W. Taylor, (eds) Bioinformatics, sequences, structures and databanks. Oxford University Press, pp: 157–185.

8. Gupta R, Vairale MG, Deshmukh RR, Chaudhary PR and SR Vate (2010) Ethnomidicinal uses of some plants used by Gond tribe of Bhandara Distrtict, Maharashtra, 9 (4):713-717.

9. Hopkinson DA and H Harris (1968) A thrird phosphoglucomutase locus in man. Ann. Hum. Genet., 31, 359-367.

10. Kim KJ, Jansen RK, Wallace RS, Micheals HH and JD Palmer (1992) Phylogenetic implications of rbcL sequence variation in the Asteraceae. Annals of the Missouri Botanical Garden, 79: 428 – 445.

11. Kim KJ and RK Jansen (1995) ndhF sequence evolution and the major clades in the sunflower family. Proc. Natl. Acad. Sci. USA, 92: 10379-10383.

12. Panero JL and VA Funk (2002) Toward a phylogenetic subfamilial classification for the Compositae (Asteraceae). Proc. Biol. Soc. Wash., 115: 909-922.

13. Paula AV, Luisa LC, Arsenio MF and S Isabel (2000) Identification of the pgmG gene, Encoding a bifunctional protein with phosphoglucomutase and phosphomannomutase activities, in the Gellan gum–producing strain Sphingomonas paucimobilis ATCC31461. Applied and Environmental Microbiology, 66(5): 2252-2258.

14. Rastogi RS, Mendirutta N and P Rastogi (2003) Bioinformatics, Concepts, Skill and applications, CBS Publishers and distributers New Delhi (India).

15. Rattazzi MC, Scandalios JG, and GS Whitt (1983) Isozymes, current topics in biological and medical research: Genetic and evolution 10, Alan R. Liss, New York.

16. Tang YL, Ren WW, Zang L, and KX Tang (2011) Molecular cloning and characterization of gene coding for γ-tocopherol methyltransferase from lettuce (Lactuca sativa). Genet. Mol. Res., 10(4): 3204-3212.

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Published

2013-03-31

How to Cite

Thakre Swapnil B. (2013). Divergence analysis of Phosphoglucomutase from two members of family Asteraceae by applying computational tools. International Journal of Life Sciences, 1(1), 42–45. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1023