Receptor mediated counteraction of melatonin in splenocytic proliferation of lipopolysaccharide challenged mice
Abstract
The activation of the spleen lymphocyte can be measured by spleen lymphocytic proliferation assay. The pineal gland secretion melatonin hormone is well known for its immune modulation in mammalian physiology. This hormone performs the various activities including the immune responsiveness through binding of its two receptors MT1 and MT2. The aim of the present study is to focus on the MT1 and MT2 receptor mediated impacts of melatonin in bacterial toxic component LPS affected spleen lymphocytic proliferation of Swiss Albino mice. Immunohisto-chemical study revealed the localization of melatonin receptors on spleen. The western blotting analysis was done to carry out the melatonin receptors expression in spleen and splenocytes proliferation was assayed for analyzing the splenocytes proliferation during different experimental situations. The increased expression of melatonin receptors of spleen and the decreased proliferation of splenocytes were observed due to melatonin treatment in LPS challenged mice. The observing results finally conclude that melatonin counteracted the splenocytes proliferation through activation of its MT1 and MT2 receptors in spleen of LPS challenged mice.
Keywords: Melatonin; Splenocytes proliferation; LPS; MT1 and MT2 receptors.
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Copyright (c) 2020 Laskar P, Singh SS
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