Bioremediation of Chlorpyrifos Using Bacteria Isolated from Pesticide Contaminated Soil

Authors

  • Wahida Rehman The Institute of Science, Mumbai, 15, Madam Cama Road, Mumbai 400032.
  • Khan SJ The Institute of Science, Mumbai, 15, Madam Cama Road, Mumbai 400032.

Keywords:

Chlorpyrifos, toxicity, bacteria, degradation and clean up technology

Abstract

Chlorpyrifos, used as an agricultural pesticide is a cause of great environment pollution and toxicity. Hence exploration of various chlorpyrifos degrading organisms to clean up the pollutant is of immense importance. Present study shows the great efficiency of bacteria in degradation of chlorpyrifos from minimal media where chlorpyrifos is the sole carbon source available for their growth. Four bacterial species isolated from chlorpyrifos contaminated soil showed growth in simulated conditions. These bacterial species were further checked for the extent of degradation. All the four showed degradation above 70%. The most efficient one Achromobacter spp. was further studied in detail for degradation along with parallel study of bacteria Pseudomonas aeruginosa previously known for degradation capability. Both these species showed degradation above 80%. Selected bacterial species showed promising result in degradation which can be harnessed in clean up technology for chlorpyrifos contaminated environment.

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References

1. Abo-Amer Aly E (2011) Biodegradation of diazinon by Serratia marcescens DI101 and its use in bioremediation of contaminated environment, J. Micro & Biotechl., 21 (1), 71–80.

2. Bhagopaty RK, Joshi SR, Mallick A (2007) Microbial degradation of organophosphorous pesticide: Chlorpyrifos. The Internet Journal of Microbiology,4:1

3. Singh Brajesh K, Allan Walker, et al (2003) Effects of Soil pH on the Biodegradation of Chlorpyrifos and Isolation of a Chlorpyrifos-Degrading Bacterium: App. & Environ. Microbio., 69 (9), 5198–5206.

4. Cho CMH, Mulchandani A & Chen W (2002) Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agent: Appl. Env.. Microbio., 68, 2026–2030.

5. Getzin LW (1981) Degradation of chlorpyrifos in soil: influence of autoclaving, soil moisture, and temperature: J. Econ. Entomol., 74, 158–162.

6. Metin Diurak and Ferda Ukazanici (2001). Effect of Some Organophosphorus Insecticides on Soil Microorganisms: Turk J Biology, 25, 51-58.

7. McDaniel CS, Harper LL, Wild JR (1988) Cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase. J. Bacteriol. 170: 2306-2311

8. Racke KD, Coats JR (1990) Pesticides in soil microbial ecosystems. Am. Chem. Soc. Symp. Ser., 426.1-12

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Published

2013-11-30

How to Cite

Wahida Rehman, & Khan SJ. (2013). Bioremediation of Chlorpyrifos Using Bacteria Isolated from Pesticide Contaminated Soil. International Journal of Life Sciences, 97–100. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1101

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Section

Research Articles