Mutagenic effectiveness and efficiency of individual and combination treatments of EMS and gamma rays in Isabgol (Plantago ovata Forsk.)

Authors

  • Mishra Monika Cytogenetics and Mutation Breeding Research Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202 002, Uttar Pradesh, India
  • Khan Ainul Haq Cytogenetics and Mutation Breeding Research Laboratory, Department of Botany, Aligarh Muslim University, Aligarh-202 002, Uttar Pradesh, India

Keywords:

Isabgol, EMS, gamma rays, mutagenic effectiveness and efficiency, chlorophyll mutations, biological damage

Abstract

Mutagenic effectiveness and efficiency of individual and combination treatments of EMS and gamma rays were assessed in Isabgol (Plantago ovata Forsk.) var. Mayuri. The experiment included total 16 mutagenic treatments (6 each for individual treatments of EMS and gamma rays and 4 combination treatments), selected on the basis of their LD50 values. Effectiveness and efficiency were calculated on the basis of biological damage of M1 generation and chlorophyll mutation frequency of M2 generation. While mutagenic effectiveness was found to be decrease with the increasing concentrations/doses of the mutagens, mutagenic efficiency showed variable trend depending upon the mutagen type and the criteria selected for its estimation. EMS treatments showed high mutagenic effectiveness than the gamma rays and combination treatments.

Downloads

Download data is not yet available.

References

Badere RS and Chaudhary AD (2007) Effectivity and efficiency of gamma rays, sodium azide and ethlymethane sulphonate in linseed. Bioinfolet., 4: 181-187.

Bhosle SS and Kothekar VS (2010) Mutagenic efficiency and effectiveness in cluster bean (Cyamopsis tetragonoloba (L.) Taub.). Journal of Phytology, 2(6): 21-27.

Blixt S (1970) Studies on induced mutations in peas. XXVI. Genetically controlled differences in radiation sensitivity. Agric. Hort. Genet., 28: 55-116.

Brown D (1995) Encyclopedia of Herbs and Their Uses. pp: 331, Dorling Kindersley, London.

Cheema AA and Atta BM (2003) Radiosensitivity studies in Basmati rice. Pak. J. Bot., 35(2): 197-207.

Desai RM and Bhatia CR (1975) Mutagenecity of N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea in durum wheat. Mutation Res., 27: 119-121.

Dhanavel D, Pavadai P, Mullainnathan L, Mohana D, Raju G, Girija M and Thilagavathi C (2008) Effectiveness and efficiency of chemical mutagens in cowpea (Vigna unguiculata (L.) Walp.). Afr. J. Biotechnol., 7: 4116-4117.

Dixit P and Dubey DK (1986) Mutagenic efficiency of gamma rays, NMU and their combinations in lentil (Lens culinaris Medik) var. T 36. Indian J. Genet., 46(3): 501-505.

Gaikwad NB and Kothekar VS (2004) Mutagenic effectiveness and efficiency of ethylmethane sulphonate and sodium azide in lentil (Lens culinaris Medik.). Indian J. Genet., 64(1): 73-74.

Girija SP and Apparao J (2011) Studies on effectiveness and efficiency of EMS in pigeonpea (Cajanus cajan L.). Bioscience Discovery, 2: 29-31.

Gupta PK and Yashvir AN (1975) Induced mutations in foxtail millet (Setaria italica Beauv.). I. Chlorophyll mutations induced by gamma rays, EMS and DES. Theor. Appl. Genet., 45: 242-249.

Khadke SG (2005) Genetic improvement of mothbean through mutation breeding. Ph.D. Thesis, Dr. BA Marathwada University, Aurangabad, M.S., India.

Khan MH and Tyagi SD (2010) Studies on effectiveness and efficiency of gamma rays, EMS and their combination in soybean (Glycine max (L.) Merrill). J. Plant Breed. Crop Sci., 2: 55-58.

Kharkwal MC (1998) Induced mutations in chickpea (Cicer arietinum L.). I. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens. Indian J. Genet., 58(2): 159-167.

Konzak CF, Nilan RN, Wagner J and Foster RJ (1965) Efficient chemical mutagenesis. In: The use of induced mutation in plant breeding. Rad. Bot. (Suppl.), 5: 49-70.

Kumar DS, Nepolean T and Goplan A (2003) Effectiveness and efficiency of the mutagens gamma rays and ethylmethane sulphonate on limabean (Phaseolus lunatus L.). Indian J. Agric. Res., 37(2): 115-119.

Kumar PRR and Ratnam SV (2010) Mutagenic effectiveness and efficiency in varieties of sunflower (Helinthus annuus L.) by separate and combined treatments with gamma rays and sodium azide. African J Biotecnol., 9(39): 6517-6521.

Kundi RS, Gill MS, Singh TP and Phul PS (1997) Radiation induced variability for quantitative trairs in soybean (Glycine max L. Merrill). Crop Improv., 24(2): 231-234.

Mishra D and Singh B (2014) Studies on effectiveness and efficiency of gamma rays in green gram (Vigna radiate (L.) Wilczek). SABRAO J. Breed. Genet., 46(1): 34-43.

Nerkar YS (1977) Mutagenic effectiveness and efficiency of gamma rays, ethyl methanesulphonate and nitroso methyl urea in Lathyrus sativus. Indian J. Genet., 37(1): 137-141.

Panchabhaye PM (1997) Mutation breeding of sunflower (Helianthus annuus L.). Ph.D. Thesis, Dr. BA Marathwada University, Aurangabad, M.S., India.

Pavadai P, Girija M and Dhanavel D (2009) Effectiveness, efficiency and biochemical content of physical and chemical mutagens in soybean (Glycine max (L.) Merrill). J. Phytol., 1: 444-447.

Sareen S, Kaul V and Koul AK (1999) Resource allocation in induced variants of Plantago ovata Forsk. Crop Improv., 26(1): 38-45.

Satpute RA and Fultambkar RV (2012) Effectiveness and efficiency of the mutagens gamma rays and EMS in soybean (Glycine max (L.) Merrill)., Curr. Bot., 3(2): 18-20.

Shah TM, Mirza JI, Haq MA and Atta BM (2008) Induced genetic variability in chickpea (Cicer arietinum L.). II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens. Pak. J. Bot., 40(2): 605-613.

Sharma SK and Sharma B (1981) Induced chlorophyll mutations in lentil. Indian J. Genet., 41(3): 328-333.

Sharma SK, Sood R and Pandey DP (2005) Studies on mutagen sensitivity, effectiveness and efficiency in urdbean (Vigna mungo (L.) Hepper.). Indian J Genet., 65: 20-22.

Singh AK and Singh RM (2007) Gamma rays and EMS induced chlorophyll mutations in mungbean (Vigna radiata (L.) Wilczek.). Indian J. Crop Sci., 2(2): 355-359.

Thakur JR and Sethi GS (1995) Comparative mutagenicity of gamma rays, ethylemethane sulphonate and sodium azide in barley (Hordeum vulgare L.). Crop Res., 9: 350-357.

Waghmare VN and Mehra RB (2001) Induced chlorophyll mutations, mutagenic effectiveness and efficiency in Lathyrus sativus L. Indian J. Genet., 60(1): 53-56.

Downloads

Published

2014-06-30

How to Cite

Mishra Monika, & Khan Ainul Haq. (2014). Mutagenic effectiveness and efficiency of individual and combination treatments of EMS and gamma rays in Isabgol (Plantago ovata Forsk.). International Journal of Life Sciences, 2(3), 212–216. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1158

Issue

Vol. 2 No. 3 (2014): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2014 Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/