Effect of Gamma Irradiation on Floral Morphology and Pollen Viability in Two Varieties of Pea (Pisum sativum L.)

Authors

  • Aney AK Shri Shivaji Education Society Amravati’s Science College, Pauni, Dist. Bhandara-441 910, (M.S.) India

Keywords:

Floral characters, pollen viability, irradiation, sensitivity, mutagens, genotype

Abstract

Mutation breeding by using radiations has been proved to be an important tool in introducing different desirable characters of agronomic values. Irradiation of plant materials by gamma rays is widely used to induce mutations at the genetic level which encounter with number of biochemical processes leading to the desirable changes in the genotype. Seeds of two varieties of Pea i.e. P. sativum var. hortense (garden pea) and P. sativum var. arvense (field pea) were exposed to different doses of gamma irradiation to evaluate the effect on morphological, floral characters and pollen viability. Gamma irradiation mostly has adverse effect on floral characters and pollen viability. It has been apparent from the data that different floral parts in both the varieties are showing dose dependent reducing trends. Lower doses of gamma irradiation are found to be enhancing the number of pollen grains produced in the anther. Pollen-ovule ratio has shown significant increase in var. hortense except in 200 Gy dose. Data on present studies has reveled that genotype of var. arvense is more sensitive to gamma irradiation than var. hortense.

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References

Alexander MP (1969) Differential staining of aborted and non-aborted pollen. Stain Technology, 40: 117-122

Chary SN and Bhalla JK (1988) Mutagenic effectiveness and efficiency of gamma rays and EMS on pigeon pea (Cajanus cajan (L.) Millsp.) J. Cytol. Genet., 23: 174-162

Cherian SK, Pruthi JS and Tandon GL (1955) Recent advances in the canning of processed peas. Indian Fd. Pack., 9(3): 25-30

Ehrenberg T (1961) Chromosome interchange in barley. Cytologia 19: 191-202

Farha Jabee, Mohammad YK and Ansari SD (2008) Studies on effect of maleic hydrazide on root tip cells and pollen fertility in Trigonella foenum-graecum L., Turk J. Bot., 32:337-344.

Fierlinger P and Vik J (1966) The possibilities of using mutation processes in breeding of leguminous plants. Genetika a Slechteni 2: 87-92

Gaul H and Mittelstenscheid L (1960) Hinweise zur Herstellung von Mutationen durch ionisienende strahlen in der pflanzenzunchtung. Z. Pflazuncht, 43: 404-422

Gunckel JE and Sparrow AH (1954) Aberrant growth in plants induced by ionizing radiation. In abnormal and pathological plant growth, Brookhaven Symposia in Biol,. 6: 252-279.

Gupta MN and Samata Y (1967) The relationship between developmental stages of flower buds andsomatic mutations induced by X-ray and chronic gamma irradiation in Cosmos bipinnatus, Rad. Bot., 7: 225-240

Gupta YP and Das (1955) Amino acid content of pure strains of Indian pulses.1. Methionine, cysteine and tryptophan. Ann. Biochem., 15: 75-80

Kumar G, Keshwani S and Sharma V (2003) Clastogenic effect of individual and combined treatment of gamma rays and EMS in Lens culinaris. J. Cyto. Genet., 4 (NS):149-154

Kumar R and Mani SC (1997) Chemical mutagenesis in ‘Manhar’ variety of rice (Oryza sativa L.)Indian J. Genet., 57: 120-126

Kumar S and Dubey DK (1996) Effect of mutagens on pollen traits in M1 and their possible role as indicators of micromutations in later generations in Khesari (Lathyrus sativus L.) Var. P-505. J. Indian Bot. Soc., 75: 87-89.

Mukerji B (1957) Indigenous Indian drugs used in the treatments of diabetes. J. Sci. Industrial Res., 16 A (10): 1-16

Ramesh DV and Seetharami Reddi TVV (2002) Induced morphological variability among three genotypes of rice. J. Cyto. Genet., 3 (NS): 115-120

Rana SR and Swaminathan MS (1964) Cytological aspects of pollen sterility. Recent Advances in Palynology, National Botanical garden, Lucknow, 276-304

Ramanna MS (1974) The origin of unreduced microspores due to aberrant cytokinesis in meiocytes of potato and its genetic significance. Euphytica, 23: 20-30

Sanyal SN (1960) Ten years of research on oral contraceptive from Pisum sativum L., Science & Culture, 25 (12): 661-665.

Sinha SSN and Godward MBE (1972) Radiation studies in Lens culinaris, Meiosis: abnormalities induced due to gamma radiation and its consequences. Cytologia, 37: 685-695

Sjodin J (1971) Induced translocation in Vicia faba L. Hereditas, 68: 1-34

Sparrow AH (1961) Types of ionizing radiations and their cytogenetic effects. Mutation and Plant Breeding NAS- NRC, 891: 55-119

Tarar JL and Dnyansagar (1980) Mutation-induced variation in flowering and floral morphology in plants of Turnera ulmifolia L. J. Indian Bot. Soc., 59: 164-169

Tomkins V and Grants R (1972) Cytogenetic studies of reciprocal translocations induced by X-ray in Hordeum vulgare L. Heredity, 31: 85-94

Verma RP, Srivastava GK and Kumar G (1999) Comparative radiocytological studies in three varieties of Lens culinaris. J. Cyto. Genet., 34 (1): 49-56.

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Published

2014-06-30

How to Cite

Aney AK. (2014). Effect of Gamma Irradiation on Floral Morphology and Pollen Viability in Two Varieties of Pea (Pisum sativum L.). International Journal of Life Sciences, 2(3), 217–222. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1159