Statistical optimization of an amylase from Bacillus sp. by using response surface methodology
Keywords:
Bacillus sp, Amylase, RSMAbstract
There are four Bacillus sp. were tentatively identified by using culture dependent method. Among them one of the Bacillus sp. having potential to produce maximum extracellular amylase on skimmed milk agar plate. The production of an extracellular amylase by Bacillus sp was statistically optimized by using response surface methodology (RSM). The maximum production of amylase from Bacillus sp. was achieved in presence of glucose and peptone as a carbon and nitrogen source respectively. The obtained ANOVA from central composite design showed a significant p value hence the designed model can be useful for to produce amylase by Bacillus sp.
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