“Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents”

Authors

  • Sunil KCR Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Swati K Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Bhavya G Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Nandhini M Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Veedashree M Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Prakash HS Eco-Biotech Laboratory, Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysuru, India – 570 006
  • Kini KR “Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents”
  • Geetha N “Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents”

Keywords:

Heavy Metals, Minimal Glucose Yeast Extract (MGY), Industrial effluents, Agricultural lands, Metal-tolerant bacteria

Abstract

Prime drive of the research work carried out was to explore heavy metal-tolerant bacteria from rhizospheric soil samples collected from contaminated agricultural lands of southern India. Soil samples of Mysore agricultural lands of Karnataka state, India, irrigated with industrial effluents from several decades, were brought to the laboratory in sterile polythene bags and were screened by plating serially diluted samples on to Minimal Glucose Yeast extract agar media amended with 0.3mM mercury. Morphologically differing cultures were tested for their tolerance to mercury by growing them in the liquid minimal media amended with 0.3, 0.4, 0.5 and 0.6mM concentrations of mercuric chloride and 0.5, 1.0 and 1.5mM of lead nitrate at optimal physiological conditions. Further the isolates were identified using 16s r-DNA PCR amplification. Blast analysis of the sequence results revealed for the first time the tolerance potential of Streptomyces flavomacrosporus, Bacillus methylotrophicus, Achromobacter xylosoxidans, Bacillus tequilensis, Bacillus pumilus, and Bacillus subtilis and their chances of participation in bioremediation of mercury. Among these isolates the actinomycetes S. flavomacrosporus, showed tolerance to multi metals.

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Published

2015-03-30

How to Cite

Sunil KCR, Swati K, Bhavya G, Nandhini M, Veedashree M, Prakash HS, Kini KR, & Geetha N. (2015). “Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents”. International Journal of Life Sciences, 3(1), 9–15. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1199

Issue

Vol. 3 No. 1 (2015): International Journal of Life Sciences

Section

Research Articles

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