Effect of temperature on the antimicrobial activity of lime juice-honey syrup on certain bacterial isolate

Authors

  • Unegbu Chika C Department of chemistry/biochemistry, Federal Polytechnic Nekede, Owerri, Imo State, Ngeria
  • Njoku SC Department of chemistry/biochemistry, Federal Polytechnic Nekede, Owerri, Imo State, Nigeria
  • Ajah Obinna Department of Pharmaceutical Technology, Federal Polytechnic Nekede, Owerri, Imo State, Nigeria.
  • Nnaoma IE Department of Pharmaceutical Technology, Federal Polytechnic Nekede, Owerri, Imo State, Nigeria.

Keywords:

Antibacterial, bacteria isolate temperature, lime juice and honey

Abstract

Traditionally in Nigeria, lime juice (Citrus aurantifolia) is added to honey and often used as a cough medicine. The present study is aimed at determining the effect of the temperature on the mixture of lime juice and honey and its antimicrobial activity on selected bacteria isolates. The lime juice is squeezed out of the fruit and then mixed with honey in the ratio of 1:1, 2:1 and 1:2 respectively, the mixture are then heated for 15minutes at various temperature (40, 60, 80 and 100oC). The result shows that the mixture still has antibacterial activity on heating. The heating temperature of 40oC, 60oC and 80oC at the ratio of 1:1 and 2:1 showed zones of inhibition when compared with the unheated but the heating temperature of 100oC has no zone of inhibition on the isolates. This indicates that heating can affect the antibacterial activity of the mixture and the evidence suggests that the lime is majorly responsible for the antibacterial activity.

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References

Anderson RC, Tomita G, Wlaker S, Merkel R, Pinchak WE and Min BR (2008) Comparative antimicrobial activity of tannin extracts from perennial plants on mastitis pathogens, scientific. Research and Essay, 3(2),66-73.

Boukraa IE (2010) Honey in traditional and modern medicine, CRe press Taylor and Francis group.

Cohen HA, Rozen J, Kristal H, Laks Y, Berkoritch M, Uziel Y, Kozer E, Pomeranz A, Efrat H, (2000) Effect of honey on nocturnal cough and sleep quality: A double¬blind randomized placebo controlled study. Pediatrics, 1-8.

De Castillo MC, De Alloric C, De Guteieirez BC (2000) Bactericidal activity of lemon juice and lime against vibrio cholera biological pharmacy. 23: 1235-1238.

Frolov VM and Peressadin NA (2006) Honey against influenza and sore throat, pcelovodstvo10 (529), 52- 53.

Owhe-Ureghe UB, Ehwarieme DA and Eboh DO (2010) Antibacterial activity of garlic and lime on isolates of extracted carious teeth, African journal of Biotechnology, 9(21) 3163-66

Stephen HJ (2004) Evaluation of honey impregnated tulle dressing in primary care. Britain Journal community Nurs; wound care supplement 2:521-527.

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Published

2016-04-11

How to Cite

Unegbu Chika C, Njoku SC, Ajah Obinna, & Nnaoma IE. (2016). Effect of temperature on the antimicrobial activity of lime juice-honey syrup on certain bacterial isolate. International Journal of Life Sciences, 4(1), 57–61. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1273

Issue

Vol. 4 No. 1 (2016): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2016 Authors

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