Effect of copper sulphate on the regulation of nitrogen metabolism in the Rita rita fish
Keywords:
Copper sulphate, Rita rita, 96 hrs LC50Abstract
Effect of copper sulphate (CuSO4.5H2O) to Rita rita (catfish) species was investigated using toxicity index of 96 hours LC50 and the quantal response determined by the statistical probit analysis method. In response to the lethality of the copper toxicant, behavioral anomalies (locomotor response) of the exposed fish species were studied as indication of toxic effects of the heavy metal. Fish species shows different mortality responses to the varying concentrations of copper sulphate studied (10 ppm to 50 ppm) due to toxicity. Copper was significantly (no overlap in 95% C.L of 96 hrs LC50 values) more toxic to Rita rita fish. 96 hrs LC50 values were revealed to be 34 mg/l. On termination of the experimental period (7 days, 15 days and 30 days) the biochemical analysis: tissue protein; total free amino acids; ammonia; urea and glutamic acid. There is need to control the use of copper because of its observed toxicity and fish avoidance test shows to be an important predictive and sensitive biomarker in aquatic monitoring and pollution management.
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