Anatomical markers and Phytochemical study of different plant parts of Bacopa monnieri (L.) Wettst.

Authors

  • Varshney Anju Department of Botany, Birla College of Arts, Science and Commerce, Kalyan (W), Maharashtra – 421304 , India
  • Chandra Naresh Principal Birla College of Arts, Science and Commerce, Kalyan (W), Maharashtra – 421304 , India
  • Patil Avinash Vice Principal, Birla College of Arts, Science and Commerce, Kalyan (W), Maharashtra – 421304 , India

Keywords:

Bacopa monnieri (L.) Wettst, foliar idioblast, anomocytic, diacytic types of stomata, prismatic calcium oxalate crystal and phytoconstituents

Abstract

Bacopa monnieri (L.) Wettst. (Scrophulariaceae) commonly known as “brahmi”, has been used in the traditional systems of medicines as "Medhya Rasayana" as it increases mental clarity and brain stimulating action. It is also being used as anti-inflammatory, analgesic, antipyretic, sedative, antioxidant and anti-cancerous agent. The present study was carried out to provide characteristic anatomical markers for root, stem and leaf, along with this study preliminary photochemical analysis was also carried out for the presence of major secondary metabolites which can act as the indicator of bioactivity of the different plant parts of Bacopa monnieri (L.) Wettst. The distinguishing features for root and stem are simple starch grains, xylem vessel with spiral thickenings, pitted tracheids and for leaf are sub- epidermal foliar idioblast, sessile, multicellular trichome and prismatic calcium oxalate crystals. Presence of two types of stomata i.e. anomocytic and diacyitc in the same field of vision under 45X magnification, are seen in the surface preparation as well as in the powder microscopy of Bacopa monnieri leaf. This is the major observation of the study. All these distinguishing features can be used as anatomical markers for correct indentification of root, stem and leaf of the plant. Preliminary phytochemical analysis revealed the presence of alkaloids, saponins, flavonoids, tannins, carbohydrates, proteins and anthroquinones in different plant parts of Bacopa monnieri while quantitative estimation showed that alkaloids (mg /g dry weight) in root, stem and leaf were found to be 1.67 ± 0.577, 47.00 ± 0.81 and 53.07 ± 2.08 respectively; saponins (mg /g dry weight) in root, stem and leaf were 7.50 ± 0.71, 23.33 ± 1.53 and 57.00 ± 2.65 respectively and flavonoids (mg /g dry weight) in stem and leaf were 25.33 ± 1.53 and 27.67 ± 2.08 respectively. Thus the findings of the present study will provide referential pharmaco - botanical information for correct identification and authentication of the crude drug and its plant parts.

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References

Agro - technique of selected medicinal plants. National Medicinal Plants Board (NMBP) (2008) Ayush, Ministry of Health and Family Welfare. TERI Press, 1: 33-37.

Banso A. (2009) Phytochemical and antimicrobial investigaton of bark extracts of Acacia nilotica. Journal of Medicinal Plants Research, 3(2): 82-85.

Coopoosamy RM and Naidoo KK (2011) Anatomical features of the medicinal importance of Aloe excelsa (Berger). African Journal of Biotechnology,10 (39): 7622- 7632.

Harborne JB. (2007) Phytochemical Methods – A Guide to Modern Technique of Plant Analysis. Chapman and Hall London, UK. Third Indian Reprint, ISBN No. 978- 81-81- 8128- 310- 8.

Gohil KJ and Patel J A (2010) A review on Bacopa monnieri: Current research and future prospects. Int J Green Pharm, 4 (1): 1-9.

Krishnaiah D, Devi T, Bono A and Sarbatly (2009) Studies on phytochemical constituents of six Malaysian medicinal plants. Journal of Medicinal Plants Research, 2: 067-072.

Khan MA, Qureshi A R, Ullah F, Gilani AS, Nosheen A, Sahreen S, Laghari K M, Laghari U M, Rehman US, Hussain I and Murad W. (2011) Phytochemical analysis of selected medicinal plants of Margalla Hills and surroundings. Journal of Medicinal Plants Research, 5(25): 6017 – 6023.

Krishnamurthy KH. Traditional Family Medicine. Isabgol, Gokhru and Brahmi. Health Series, 56-75.

Lersten RN and Curtis DJ. (1997) Anatomy and distribution of foliar idioblasts in Scrophularia and Vervsscum (Scrophulariaceae). American Journal of Botany, 84(12): 1638-1645.

Mallikharjuna PB, Rajanna NL, Seetharam NY and Sharanabasappa, KG (2007) Phytochemical Studies of Strychnos potatorum L.f. - A Medicinal Plant. E- Journal of Chemistry, 4 (4): 510-518.

Mahesh, B and Satish, S. (2008) Antimicrobial activity of some important Medicinal Plant against plant and human pathogens. World Journal of Agricultural Sciences, 4 (S): 839-843.

Modi DC, Patel JK, Shah BN and Nayak BS(2010) Pharmacognostic studies of the seed of Syzygium cumini Linn. International Journal of Pharmaceutical Sciences, 1(1): 20- 26.

Monograph (2004) Bacopa monnieri. Alternative Medicine Review, 9(1): 79-84.

Patil AG, Joshi KA, Patil DA and Naresh Chandra (2011) Pharmacognostical standardization and HPTLC fingerprint of Cardiospermum halicacum L. stem. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2(2): 343- 352.

Poornima N, Umarajan KM and Babu K (2009) Studies on anatomical and phytochemical analysis of Oxystelma esculentum (L.f.) R.br. Ex Schltes. Botany Research International, 2(4):239-243.

Prasad R, Bagde US, Puspangadan P and Varma A (2008) Bacopa monnieri (L.): Pharmacoloigcal aspects and case study involving Piriformospora indica. International Journal of Integrative Biology, 3(2): 100-110.

Russo A and Borrelli F. (2005) Bacopa monnieri, a reputed nootropic plant: an overview. International Journal of Phytotherapy and amp Phytopharmacology,12(4): 305-317.

Serrano R, Silva DG and Silva O (2010) Application of Light and Scanning Electron Microscopy in the identification of Herbal Medicines. Microscopy: Science, Technology, Application and Education. A. Mendez- Vilas and J. Diaz (Eds.), 182- 190.

Srinivasa RB, Sharan S V and Chippada S C. (2011) A Review on Pharmacological Studies of Bacopa monnieri. Journal of Chemical, Biological and Physical Sciences, 1(2): 250-259.

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Published

2017-09-19

How to Cite

Varshney Anju, Chandra Naresh, & Patil Avinash. (2017). Anatomical markers and Phytochemical study of different plant parts of Bacopa monnieri (L.) Wettst. International Journal of Life Sciences, 5(3), 379–386. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1406