Molecular characterization of Infectious Bursal Disease virus from gamefowls in selected areas in CALABARZON, Philippines

Authors

  • Caceres Abbegaile B DVM
  • Umali Dennis V DVM, PhD Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines, Los Baños, Laguna, 4031, Philippines
  • Bernardo Francis Andrew Eugene M DVM, MS Molecular characterization of Infectious Bursal Disease virus from gamefowls in selected areas in CALABARZON, Philippines
  • Katoh Hiromitsu DVM, PhD Poultry Products Quality Control (PPQC), 125-7 Daiwa Dakeonsen, Nihonmatsu, Fukushima, 964-0062, Japan

Keywords:

Calabarzon, gamefowl, Infectious bursal disease, phylogenetic analysis, vvIBD

Abstract

Infectious Bursal Disease (IBD) is a highly infectious, economically important, immunosuppressive viral disease of poultry. In this study, four field strains of IBD virus (IBDV) from gamefowls in selected areas in CALABARZON, Philippines were characterized. Clinical signs observed in affected chickens were inappetence, white scours, ruffled feathers, lethargy and acute increased in mortalities. Gross lesions observed were enlarged hemorrhagic cream colored bursae with yellowish exudates. Hemorrhages in the junction of the proventriculus and gizzard were also noted. Nucleotide sequencing showed that the field strains belong to serotype-1 with deduced amino acid sequences of 222A, 256I, 294I, 242I and 299S at the VP-2 gene, which indicates that the field strains were from the very virulent IBDV (vvIBDV) genotype. Phylogenetic analysis showed that the field strains belong to the European-like vvIBDV subgenotype and were closely related to (97-98%) to Spanish, South African and Nigerian IBDV strains. At present, this is the first molecular characterization of IBDVs in the Philippines.

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References

Adamu J, Owoade A, Abdu PA, Kazeem H and Fatihu M (2013) Characterization of field and vaccine infectious bursal disease viruses from Nigeria revealing possible virulence and regional markers in the VP2 minor hydrophilic peaks. Avian Pathology 42(5): 420-433.

Cardoso de Paula MB, Yokosawa J, Coutinho MDB, Silva PL, Ferraz RA and Oliveira TFM (2004) Identification and Molecular Characterization of the Infectious Bursal disease virus (IBDV) from an outbreak in a broiler flock in Midwestern Brazil. Brazilian Journal of Microbioliogy 35:352-358.

Cortey M, Bertran K, Toskano J, Majo N and Dolz R (2012) Phylogeographic distribution of very virulent infectious bursal disease virus isolates in the Iberian Peninsula. Avian Pathology 41(3): 277-284.

Eterradossi N, Arnauld C, Tekaia F, Toquin D, Le Coq H, Rivallan G, Guittet M, Domenech J, van den Berg, TP and Skinner, MA (1999) Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate. Avian Pathology 28: 36-46.

Jackwood DJ, Sreedevi B, Lefever LJ and Sommer-Wagner SE (2008) Studies on naturally occurring infectious bursal disease viruses suggest that a single amino acid substitution at position 253 in VP2 increases pathogenicity. Virology 377: 110-116.

Kim HR, Kwon YK, Bae YC, Oem JK and Lee O (2010) Genetic Characteristics of virion protein 2 genes of infectious bursal disease viruses isolated from commercial chickens with clinical disease in South Korea. Poultry Science 89: 1642-1646.

Lone NA, Rehmani SF, Kazmi SU, Muzaffat R, Khan TA, Khan A, Khan SA and Ahmed A (2009) Molecular Characterization of Pakistani Field Isolates of Infectious Bursal Disease Virus. Avian Diseases 53: 306-309.

Mawgod SA, Arafa AS and Hussein HA (2014) Molecular genotyping of the infectious bursal disease virus (IBDV) isolated from Broiler Flocks in Egypt. International Journal of Veterinary Science and Medicine 2: 46-52.

Mittal D, Jindal N, Gupta SL, Tawari AK (2005) Detection of Infectious Bursal Disease Virus in Field Outbreaks in Broiler Chickens by Reverse Transcription-Polymerase Chain Reaction. International Journal of Poultry Science 4: 239-243.

Mohamed MA, Elzanaty KES, Bakhit M and Safwat M (2014) Genetic Characterization of Infectious Bursal Disease Viruses Associated with Gumboro Outbreaks in Commercial Broilers from Asyut Province, Egypt. ISRN Veterinary Science Article ID 916412, 9 pages; doi:10.1155/2014/916412

Ozbey G, Ertas HB and Mutz A (2003) Restriction fragment length polymorphism analysis of isolates of infectious bursal disease viruses from Turkey. Vet. Med/ Czech 48(12): 359-362.

Roussn DA, Al-Saleh W, Khawaldeh G and Totanji WS (2012) Characterization of infectious bursal disease virus field strains in Jordan using molecular techniques – a short communication. Veterinarski Arhiv 82(1): 115-124.

van den Berg, TP, Eterradossi N, Toquin D and Meulemans G (2000) Infectious Bursal Disease (Gumboro disease). Rev. Sci. Tech. Off. Int. Epiz. 19(2): 527-543.

van den Berg TP, Morales D, Eterradossi N, Rivalian G, Toquin D, Rauec R, Zierenberg K, Zhang MF, Zhud YP, Wange CQ, Zheng H, Wang JX, Chen GC, Ling BL and Müller H (2004) Assessment of genetic, antigenic and pathotypic criteria for the characterization of IBDV strains. Avian Pathology 33(5): 470-476.

Yamaguchi T, Kasanga CJ, Terasaki K, Maw MT, Ohya K and Fukushi H (2007) Nucleotide Sequence Analysis of VP2 Hypervariable Domain of Infectious Bursal Disease Virus Detected in Japan from 1993 to 2004. Journal of Veterinary Medical Science 69(7): 733-738.

Zahoor MA, Abubakar M, Naim S, Khan QM and Arshed MJ (2011) Molecular Typing of Field Isolates from two outbreaks of Infectious Bursal Disease Virus from Pakistan. Veterinary World 4(7): 297-300.

Zahoor M, Abubakar M, Naim S, Khan Q and Arshed MJ (2010) Incidence and Molecular Characterization of Infectious Bursal Disease Virus in Commercial Broilers in Pakistan. IJAVMS 4(3): 75-80.

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Published

2017-11-21

How to Cite

Caceres Abbegaile B DVM, Umali Dennis V DVM, PhD, Bernardo Francis Andrew Eugene M DVM, MS, & Katoh Hiromitsu DVM, PhD. (2017). Molecular characterization of Infectious Bursal Disease virus from gamefowls in selected areas in CALABARZON, Philippines. International Journal of Life Sciences, 5(3), 491–498. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1424

Issue

Vol. 5 No. 3 (2017): International Journal of Life Sciences

Section

Research Articles

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Copyright (c) 2017 Authors

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