Study of Anti-psoriatic activity and Evaluation of E2A Gene Expression for Psoriasis Levels by RT-PCR of Thespesia populnea L. Methanolic Leaf Extract

Authors

  • Narendar Vankudothu Department of Genetics, University College of Science, Osmania University, Hyderabad, Telangana-500007, India.
  • Chandrashekhar R Pharmacognosy and Plant Molecular genetics Lab, Department of Botany, University College of Science, Saifabad, Osmania University, Hyderabad, Telangana-500004, India.
  • Sudhakar Chekuri Department of Genetics, University College of Science, Osmania University, Hyderabad, Telangana-500007, India.
  • Lakshmi Bhavani N Pharmacognosy and Plant Molecular genetics Lab, Department of Botany, University College of Science, Saifabad, Osmania University, Hyderabad, Telangana-500004, India.
  • Anwar SY Department of Genetics, University College of Science, Osmania University, Hyderabad, Telangana-500007, India.

Keywords:

Thespesia populnea L., Anti-psoriatic activity, Phytoconsituents, Retion-A (0.05%)

Abstract

Thespesia populnea L. (Malvaceae) traditionally claimed to be constructive in the cure of cutaneous infections such as scabies, psoriasis, ringworm, guinea worm, eczema and herpetic diseases. Oil prepared by boiling the ground bark and leaves in coconut oil is applied externally in psoriasis and scabies. Conversely there are no customary scientific reports for its anti-psoriatic activity. Hence the plant Thespesia populnea has been chosen to establish scientific data for its traditional claim as anti-psoriatic. In the present study a chemical test performed against all the phytochemical constituents for their preliminary screening of each solvent extract of leaf like water, methanol, chloroform, ethyl acetate, pet ether and acetone. From the phytochemical analysis of the leaf extracts; the result infers the presence of glycosides, tannins, flavonoids, triterpenoids and phytosterols in all the solvent extracts. But phenols are more retained in methanolic leaf extract compared to all the phytochemical constituents. Screening for anti-psoriatic activity was carried out by topical application of methanolic leaf extract of Thespesia populnea in the form of a cream using the Perry’s scientific mouse tail model. Successive methanolic leaf extract showed paramount anti-psoriatic activity than the standard drug Retion-A (0.05%). Potential function of E2A as a biomarker in psoriasis, is applied in the current investigation to study whether the methanolic leaf extracts of Thespesia populnea L. showed antipsoriatic effect targeting E2A gene expression for the psoriasis levels. Results indicated that methanolic leaf extract of Thespesia populnea can preferably be used as anti-psoriatic drug and the plant is promising for further investigations to prove its anti-psoriatic activity with no side effects.

Downloads

Download data is not yet available.

References

Akindele AJ, Adeyemi OO (2007) Anti inflammatory activity of the aqueous leaf extract of Byrsocarpuscoccineus. Fitoterapia 2007, 78(1):25-8.

Bjorntorp E, Parsa R, Thornemo M, Wennberg AM et al. (2003) The helix-loop-helix transcription factor Id1 is highly expressed in psoriatic involved skin. Acta Derm. Venereol., 83: 403-409.

Chandran RP, Manju S, Shaji PK, Nair GA and Sukumar B (2016) In Vitro Cytotoxic Activities of Leaf Extracts of Thespesia populnea and HygrophillaSchulli Against Dalton’s Lymphoma Ascites and Ehrlich Ascites Carcino-ma Cell Lines. Austin J.Lung Cancer Res, 1(2): 1007.

Chikhali Umakant, Shankar Shinde, Amar Dinkar, Patil Sandip, Balvant Naikwade (2011) Evaluation of antitumor, antioxidant and cytotoxic activity of Thespesia populnea bark extract. Journal of Pharmacy Research, 4(10):3641

Engel I and Murre C (2001) The function of E- and Id proteins in lymphocyte development. Nat. Rev. Immunol. 1: 193- 199.

Karna P, Chagani S, Gundala SR, Rida PCG, Asif G, Sharma V, Gupta MV, Aneja R (2012) Benefits of whole ginger extract in prostate cancer. British Journal of Nutrition, 107(4):473–484.

Kee BL (2009) E and ID proteins branch out. Nat. Rev. Immunol., 9: 175-184.

Lindamulage IK, Soysa P (2016) Evaluation of anticancer properties of a decoction containing Adenanthera-pavonina L. and Thespesiapopulnea. BMC Complement Altern Med., 16:70

Liu RH (2003) Health benefits of fruit and vegetables are from additive and synergistic combinations of phytochemicals. American Journal of Clinical Nutrition.78(3):517S–520S.

Mark EB, Jonsson M, Asp J, Wennberg AM et al. (2006) Expression of genes involved in the regulation of p16 in psoriatic involved skin. Arch.Dermatol.Res.,297:459-467.

Patwardhan B, Vaidhya ADB and Chorghade M (2004) Ayurveda and Naturalproducts drug discovery. Curr Sci., 86: 789-799.

Perk J, Iavarone A and Benezra R (2005) Id family of helix-loop-helix proteins in cancer. Nat.Rev.Cancer.5:603-614.

Pritam Kumar Panda, Danish Ibrahim, Pramod Kumar P. Gupta (2014) Computational modeling and analysis of theoretical structure of corneodesmosin receptor protein with existing phytochemicals in psoriasis, Indian J. of Fund. and Applied Life Sciences.2014, 4 (4), 346-355.

Parthasarathy R, Ilavarasan R, Karrunakaran CM (2009) Antidiabetic activity of ThespesiaPopulnea bark and leaf extract against streptozotocin induced diabetic rats. International J. of Pharm.Tech Research. 2009.

Samuel ML, Donald PM, Hurley JH Jr (1986) Dermatology Vol-I. W. B. Philadelphia:SaundersCompany; p.204.

Sandhya T, Lathika KM, Pandey BN, Mishra KP (2006) Potential of ayurvedic formulation, Triphala, as novel anticancer drug. Cancer letters, 231(2): 206-214.

Satyavati GV, Gupta AK, Tandon N (1987) Medicinal Plants of India. ICMR, New Delhi, India.

Siddharth Shrivastav, Rakesh K. Sindhu, Sanjeev Kumar, Pradeep Kumar (2009) Anti-Psoriatic And Phytoche-mical Evaluation Of Thespesia Populnea Bark Extracts. Int J Pharm Pharm Sci.

Si-Yuan Pan, Gerhard Litscher, Si-HuaGao, Shu-Feng Zhou, Zhi-Ling Yu, Hou-Qi Chen, Shuo-Feng Zhang, Min-Ke Tang, Jian-Ning Sun, and Kam-Ming Ko. Historical

Perspective of Traditional Indigenous Medical Practices: The Current Renaissance and Conservation of Herbal Resources. Evidence-Based Complementary and Alternative Medicine. 2014.

Walter LF, Gundula S (1981) Histopathology of the skin. 3rd Edn., Boston, Massachusetts: Lippincott;p.156-64.

Yogayata S Pathare, Vijay D Wagh(2012) Herbal Medicines and Nutritional supplements used in the treatment of Glaucoma: A Review. RJPBCS, Volume 3 Issue 1.

Downloads

Published

2018-01-07

How to Cite

Narendar Vankudothu, Chandrashekhar R, Sudhakar Chekuri, Lakshmi Bhavani N, & Anwar SY. (2018). Study of Anti-psoriatic activity and Evaluation of E2A Gene Expression for Psoriasis Levels by RT-PCR of Thespesia populnea L. Methanolic Leaf Extract. International Journal of Life Sciences, 6(1), 6–12. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1427