Arsenic induces oxidative stress and apoptosis in human lung cancer cells

Authors

  • Kiran Kumar KM Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India
  • Naveen Kumar M Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India
  • Rashmi Nagesh Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India
  • Rajeshwari H Patil Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India
  • Babu RL Department of Bioinformatics and Biotechnology, Akkamahadevi Women’s University, Jnana Shakthi Campus, Vijayapura, 586 108, Karnataka, India
  • S Chidananda Sharma Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India
  • Manjunath K Department of Microbiology and Biotechnology, Bangalore University, Jnana Bharathi, Bengaluru- 560 056, Karnataka, India

Abstract

Arsenic is a toxic heavy metal and a well-known environmental contaminant. Often water, food, soil are found to be contaminated with arsenic and occupational exposure leads to harmful health issues. Hence, in the present study human lung epithelial A549 cells were used as in vitro model system. Treatment of A549 cells with arsenic for 48 h showed reduced viability with increased leakage of lactate dehydrogenase with loss of membrane damage. Arsenic significantly induces reactive oxygen species with depletion of free radical neutralizing antioxidant glutathione peroxidase and catalase enzymes. IC50 value of 2 µM arsenic concentration was used for mRNA expression studies. Further, pro-apoptotic Bax gene was induced and anti-apoptotic Bcl-2 was decreased. Arsenic stimulates dysfunction of cell cycle progression by downregulation of Cyclin-E1and Cyclin-D1 mRNA expressions. Thus, confirms the toxic nature of arsenic and clues to oxidative redox potential alteration with cell cycle inhibition and apoptosis in lung epithelial cells.

Keywords: Arsenic, A549 cells, Oxidative stress, Apoptosis, Cyclins.

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Published

2017-12-05

How to Cite

Kiran Kumar KM, Naveen Kumar M, Rashmi Nagesh, Rajeshwari H Patil, Babu RL, S Chidananda Sharma, & Manjunath K. (2017). Arsenic induces oxidative stress and apoptosis in human lung cancer cells. International Journal of Life Sciences, 5(4), 551–560. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/148

Issue

Vol. 5 No. 4 (2017): International Journal of Life Sciences

Section

Research Articles

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Copyright (c) 2017 Kiran Kumar KM, Naveen Kumar M, Rashmi Nagesh, Rajeshwari H Patil, Babu RL, S Chidananda Sharma, Manjunath K

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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