Exploring the Biological Mechanisms of Healing and Functional Recovery After Achilles Tendon Rupture Repair
DOI:
https://doi.org/10.66167/ijlsci/2025113237Keywords:
Achilles Tendon, Calf Muscle Atrophy, Tendon Elongation, Early Functional Mobilization, Achilles Tendon RuptureAbstract
The Achilles tendon is the largest and strongest tendon in the human body, capable of withstanding forces twelve times a human's body weight. It is essential for locomotion enabling efficient walking, running, and jumping. Acute tendon rupture is a debilitating injury that often requires prolonged rehabilitation. It triggers a complex healing cascade progressing through overlapping inflammatory, proliferative, and remodeling phases over months. However, tissue often forms fibrotic scars, leaving the tendon susceptible to adhesions and re-rupture. Even after surgical repair, the tendon may heal in an elongated, less stiff state. Additionally, the calf muscles frequently undergo atrophy and fatty degeneration. These structural changes in the muscle tendon unit lead to persistent plantarflexion weakness and reduced push off strength, making full functional restoration a significant challenge. This research paper examines evidence based rehabilitation strategies to enhance tendon healing and functional recovery. Early controlled mechanical loading and mobilization have been shown to promote tendon maturation and reduce re-rupture risk. Muscle preservation therapies can help with disuse atrophy. Adjunctive biological treatments, such as platelet rich plasma injections, have been explored to accelerate the healing process, though clinical outcomes remain inconclusive. By integrating insight from the biology of tendon repair and muscle protection, this research paper aims to inform more effective post rupture rehabilitation protocols that safely minimize re-injury risk and optimize functional recovery.
Downloads
References
Abate M, Gravare-Silbernagel K, Siljeholm C, Di Iorio A, De Amicis D, Salini V et al. (2009) Pathogenesis of tendinopathies: Inflammation or degeneration? Arthritis Research & Therapy 11(3): 235. https://doi.org/10.1186/ar2723
Abdul Alim M, Domeij-Arverud E, Nilsson G, Edman G, Ackermann PW (2018) Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis. Knee Surgery, Sports Traumatology, Arthroscopy 26(7): 2021–2029. https://doi.org/10.1007/s00167-017-4621-8
Adami A, Corvino RB, Calmelat RA, Porszasz J, Casaburi R, Rossiter HB (2020) Muscle Oxidative Capacity Is Reduced in Both Upper and Lower Limbs in COPD. Medicine and Science in Sports and Exercise 52(10): 2061–2068. https://doi.org/10.1249/MSS.0000000000002364
Andarawis‐Puri N, Flatow EL, Soslowsky LJ (2015) Tendon basic science: Development, repair, regeneration, and healing. Journal of Orthopaedic Research 33(6): 780–784. https://doi.org/10.1002/jor.22869
Balboni JM, Madhira K, Martinez V, Tung WS, Kennedy JG, Gianakos AL (2025) Effect of blood flow restriction on muscle strength and stability following foot and ankle injury: A systematic review. World Journal of Orthopedics 16(6): 106804. https://doi.org/10.5312/wjo.v16.i6.106804
Barfod KW, Overgård AB, Hansen MS, Haddouchi IE, Toft M, Hölmich P (2023) Effect of the Copenhagen Achilles Rupture Treatment Algorithm (CARTA) on Calf Muscle Volume and Tendon Elongation After Acute Achilles Tendon Rupture: A Predefined Secondary Analysis of the First 60 Patients in a Randomized Controlled Trial. Orthopaedic Journal of Sports Medicine 11(11): 23259671231211282. https://doi.org/10.1177/23259671231211282
Benjamin M, Kaiser E, Milz S (2008) Structure-function relationships in tendons: A review. Journal of Anatomy 212(3): 211–228. https://doi.org/10.1111/j.1469-7580.2008.00864.x
Bordoni B, Black AC, Varacallo MA (2025) Anatomy, Tendons. In: StatPearls. StatPearls Publishing.
Brumitt J, Cuddeford T (2015) CURRENT CONCEPTS OF MUSCLE AND TENDON ADAPTATION TO STRENGTH AND CONDITIONING. International Journal of Sports Physical Therapy 10(6): 748–759.
Butterfield TA, Best TM, Merrick MA (2006) The Dual Roles of Neutrophils and Macrophages in Inflammation: A Critical Balance Between Tissue Damage and Repair. Journal of Athletic Training 41(4): 457–465.
Carcia CR, Martin RL, Jeff, Wukich DK (2010) Achilles Pain, Stiffness, and Muscle Power Deficits: Achilles Tendinitis. Journal of Orthopaedic & Sports Physical Therapy 40(9): A1–A26. https://doi.org/10.2519/jospt.2010.0305
Chen K, Hu X, Blemker SS, Holmes JW (2018) Multiscale computational model of Achilles tendon wound healing: Untangling the effects of repair and loading. PLOS Computational Biology 14(12): e1006652. https://doi.org/10.1371/journal.pcbi.1006652
Contreras-Hernandez I, Arvanitidis M, Falla D, Negro F, Martinez-Valdes E (2024) Achilles tendon morpho-mechanical parameters are related to triceps surae motor unit firing properties. Journal of Neurophysiology 132(4): 1198–1210. https://doi.org/10.1152/jn.00391.2023
Darrieutort-Laffite C, Blanchard F, Soslowsky LJ, Le Goff B (2024) Biology and physiology of tendon healing. Joint Bone Spine 91(5): 105696. https://doi.org/10.1016/j.jbspin.2024.105696
Deng Z, Fan T, Xiao C, Tian H, Zheng Y, Li C et al. (2024) TGF-β signaling in health, disease and therapeutics. Signal Transduction and Targeted Therapy 9: 61. https://doi.org/10.1038/s41392-024-01764-w
DiIorio SE, Young B, Parker JB, Griffin MF, Longaker MT (2024) Understanding Tendon Fibroblast Biology and Heterogeneity. Biomedicines 12(4): 859. https://doi.org/10.3390/biomedicines12040859
Dirks ML, Wall BT, Snijders T, Ottenbros CLP, Verdijk LB, van Loon LJC (2014) Neuromuscular electrical stimulation prevents muscle disuse atrophy during leg immobilization in humans. Acta Physiologica 210(3): 628–641. https://doi.org/10.1111/apha.12200
Freedman BR, Gordon JA, Soslowsky LJ (2014) The Achilles tendon: Fundamental properties and mechanisms governing healing. Muscles, Ligaments and Tendons Journal 4(2): 245–255.
Fu SC, Rolf C, Cheuk YC, Lui PP, Chan KM (2010) Deciphering the pathogenesis of tendinopathy: A three-stages process. Sports Medicine, Arthroscopy, Rehabilitation, Therapy & Technology: SMARTT 2: 30. https://doi.org/10.1186/1758-2555-2-30
Gaines J (2021) Low-load blood flow restriction (BFR) training induces similar Achilles tendon adaptations compared with high-load resistance training. IAOM-US.
Galloway MT, Lalley AL, Shearn JT (2013) The Role of Mechanical Loading in Tendon Development, Maintenance, Injury, and Repair. The Journal of Bone and Joint Surgery 95(17): 1620–1628. https://doi.org/10.2106/JBJS.L.01004
Galvan E, Arentson-Lantz E, Lamon S, Paddon-Jones D (2016) Protecting Skeletal Muscle with Protein and Amino Acid during Periods of Disuse. Nutrients 8(7): 404. https://doi.org/10.3390/nu8070404
Gesteira TF, Verma S, Coulson-Thomas VJ (2023) Small leucine rich proteoglycans: Biology, function and their therapeutic potential in the ocular surface. The Ocular Surface 29: 521–536. https://doi.org/10.1016/j.jtos.2023.06.013
Gil-Melgosa L, Grasa J, Urbiola A, Llombart R, Susaeta Ruiz M, Montiel V et al. (2021) Muscular and Tendon Degeneration after Achilles Rupture: New Insights into Future Repair Strategies. Biomedicines 10(1): 19. https://doi.org/10.3390/biomedicines10010019
Harmon KK, Stout JR, Fukuda DH, Pabian PS, Rawson ES, Stock MS (2021) The Application of Creatine Supplementation in Medical Rehabilitation. Nutrients 13(6): 1825. https://doi.org/10.3390/nu13061825
Heikkinen J, Lantto I, Piilonen J, Flinkkilä T, Ohtonen P, Siira P et al. (2017) Tendon Length, Calf Muscle Atrophy, and Strength Deficit After Acute Achilles Tendon Rupture: Long-Term Follow-up of Patients in a Previous Study. Journal of Bone and Joint Surgery 99(18): 1509–1515. https://doi.org/10.2106/JBJS.16.01491
Hoeffner R, Svensson RB, Bjerregaard N, Kjær M, Magnusson SP (2022) Persistent Deficits after an Achilles Tendon Rupture: A Narrative Review. Translational Sports Medicine 2022: 7445398. https://doi.org/10.1155/2022/7445398
Hsu YC, Wu WT, Chang KV, Han DS, Chou LW (2017) Healing of Achilles tendon partial tear following focused shockwave: A case report and literature review. Journal of Pain Research 10: 1201–1206. https://doi.org/10.2147/JPR.S132951
Hyer CF, Berlet G, Philbin T, Bull P, Brandão R, Prissel M et al. (2021) Does Functional Neuromuscular Electrical Stimulation (NMES) Influence Calf Atrophy Following Achilles Tendon Surgery? Prospective Double-Blind Randomized Controlled Trial on the Use of Immediate Postoperative Electrical Muscle Stimulation to Preserve Muscle Function and Volume. The Journal of Foot and Ankle Surgery 60(4): 683–688. https://doi.org/10.1053/j.jfas.2020.12.005
Johnson KE, Wilgus TA (2014) Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair. Advances in Wound Care 3(10): 647–661. https://doi.org/10.1089/wound.2013.0517
Lemme NJ, Li NY, DeFroda SF, Kleiner J, Owens BD (2018) Epidemiology of Achilles Tendon Ruptures in the United States: Athletic and Nonathletic Injuries From 2012 to 2016. Orthopaedic Journal of Sports Medicine 6(11): 2325967118808238. https://doi.org/10.1177/2325967118808238
Lewis T, Cook J (2014) Fluoroquinolones and Tendinopathy: A Guide for Athletes and Sports Clinicians and a Systematic Review of the Literature. Journal of Athletic Training 49(3): 422–427. https://doi.org/10.4085/1062-6050-49.2.09
Li HY, Hua YH (2016) Achilles Tendinopathy: Current Concepts about the Basic Science and Clinical Treatments. BioMed Research International 2016: 6492597. https://doi.org/10.1155/2016/6492597
Liu X, Zhu B, Li Y, Liu X, Guo S, Wang C et al. (2021) The Role of Vascular Endothelial Growth Factor in Tendon Healing. Frontiers in Physiology 12: 766080. https://doi.org/10.3389/fphys.2021.766080
Lorenz DS, Bailey L, Wilk KE, Mangine RE, Head P, Grindstaff TL et al. (2021) Blood Flow Restriction Training. Journal of Athletic Training 56(9): 937–944. https://doi.org/10.4085/418-20
Lu J, Jiang L, Chen Y, Lyu K, Zhu B, Li Y et al. (2022) The Functions and Mechanisms of Basic Fibroblast Growth Factor in Tendon Repair. Frontiers in Physiology 13: 852795. https://doi.org/10.3389/fphys.2022.852795
Marrone W, Andrews R, Reynolds A, Vignona P, Patel S, O’Malley M (2024) Rehabilitation and Return to Sports after Achilles Tendon Repair. International Journal of Sports Physical Therapy 19(9): 1152–1165. https://doi.org/10.26603/001c.122643
Mashimo S, Nozaki T, Amaha K, Tanaka K, Kubota J, Sato H et al. (2023) Quantitative Assessment of Calf Muscle Volume, Strength, and Quality After Achilles Tendon Rupture Repair: A 1-Year Prospective Follow-up Study. The American Journal of Sports Medicine 51(14): 3781–3789. https://doi.org/10.1177/03635465231206391
Massoud EIE (2013) Healing of subcutaneous tendons: Influence of the mechanical environment at the suture line on the healing process. World Journal of Orthopedics 4(4): 229–240. https://doi.org/10.5312/wjo.v4.i4.229
Milz S, Rufai A, Buettner A, Putz R, Ralphs J, Benjamin M (2002) Three-dimensional reconstructions of the Achilles tendon insertion in man. Journal of Anatomy 200(2): 145–152. https://doi.org/10.1046/j.0021-8782.2001.00016.x
Nakamura S, Sato Y, Kobayashi T, Kaneko Y, Ito E, Soma T et al. (2020) Vitamin D protects against immobilization-induced muscle atrophy via neural crest-derived cells in mice. Scientific Reports 10(1): 12242. https://doi.org/10.1038/s41598-020-69021-y
Padilha CS, Cella PS, Salles LR, Deminice R (2017) Oral creatine supplementation attenuates muscle loss caused by limb immobilization: A systematic review. Fisioterapia Em Movimento 30: 831–838. https://doi.org/10.1590/1980-5918.030.004.AR01
Poenaru D, Sandulescu MI, Cinteza D (2022) Biological effects of extracorporeal shockwave therapy in tendons: A systematic review. Biomedical Reports 18(2): 15. https://doi.org/10.3892/br.2022.1597
Ricci V, Chang KV, Naňka O, Özçakar L (2025) Superficial retrocalcaneal bursae and nerves: From anatomy to ultrasound-guided procedures. Clinical Anatomy 38(1): 29–34. https://doi.org/10.1002/ca.24193
Salvatore L, Gallo N, Natali ML, Terzi A, Sannino A, Madaghiele M (2021) Mimicking the Hierarchical Organization of Natural Collagen: Toward the Development of Ideal Scaffolding Material for Tissue Regeneration. Frontiers in Bioengineering and Biotechnology 9: 644595. https://doi.org/10.3389/fbioe.2021.644595
Schweitzer ME, Karasick D (2000) MR Imaging of Disorders of the Achilles Tendon. American Journal of Roentgenology 175(3): 613–625. https://doi.org/10.2214/ajr.175.3.1750613
Screen HRC, Birk DE, Kadler KE, Ramirez F, Young MF (2015) Tendon Functional Extracellular Matrix. Journal of Orthopaedic Research 33(6): 793–799. https://doi.org/10.1002/jor.22818
Shamrock AG, Dreyer MA, Varacallo MA (2025) Achilles Tendon Rupture. In: StatPearls. StatPearls Publishing.
Siadat SM, Zamboulis DE, Thorpe CT, Ruberti JW, Connizzo BK (2021) Tendon Extracellular Matrix Assembly, Maintenance and Dysregulation Throughout Life. In: Halper J (ed) Progress in Heritable Soft Connective Tissue Diseases. Springer International Publishing, pp 45–103. https://doi.org/10.1007/978-3-030-80614-9_3
Stańczak M, Kacprzak B, Gawda P (2024) Tendon Cell Biology: Effect of Mechanical Loading. Cellular Physiology & Biochemistry 58(6): 677–701.
Svärd A, Hammerman M, Eliasson P (2020) Elastin levels are higher in healing tendons than in intact tendons and influence tissue compliance. FASEB Journal 34(10): 13409–13418. https://doi.org/10.1096/fj.202001255R
Tanaka T, Narazaki M, Kishimoto T (2014) IL-6 in Inflammation, Immunity, and Disease. Cold Spring Harbor Perspectives in Biology 6(10): a016295. https://doi.org/10.1101/cshperspect.a016295
Theobald P, Bydder G, Dent C, Nokes L, Pugh N, Benjamin M (2006) The functional anatomy of Kager’s fat pad in relation to retrocalcaneal problems and other hindfoot disorders. Journal of Anatomy 208(1): 91–97. https://doi.org/10.1111/j.1469-7580.2006.00510.x
Thomopoulos S, Parks WC, Rifkin DB, Derwin KA (2015) Mechanisms of tendon injury and repair. Journal of Orthopaedic Research 33(6): 832–839. https://doi.org/10.1002/jor.22806
Wang C, Fan H, Li Y, Yun Z, Zhang Z, Zhu Q (2021) Effectiveness of platelet-rich plasma injections for the treatment of acute Achilles tendon rupture: A systematic review and meta-analysis. Medicine 100(41): e27526. https://doi.org/10.1097/MD.0000000000027526
Wang R, Huang L, Jiang S, You G, Zhou X, Wang G et al. (2024) Immediate mobilization after repair of Achilles tendon rupture may increase the incidence of re-rupture: A systematic review and meta-analysis of randomized controlled trials. International Journal of Surgery 110(6): 3888–3899. https://doi.org/10.1097/JS9.0000000000001305
Wawrzyński T, Pietrzak BA, Mika A (2022) Does Mobility of the Ankle Joint Depends on Length of the Free Part of the Achilles Tendon? Symmetry 14(11): 2323. https://doi.org/10.3390/sym14112313
WebMD (2023) All About Achilles Tendon Injuries. WebMD.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Ashwin Sankar Narayanan

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/



