Antioxidative, Antibacterial and Antibiofilm Activity of Melanin nanoparticles (MN)

Authors

  • Amit Tiwari Department of Microbiology, Ramnarain Ruia Autonomous College, University of Mumbai, Mumbai, Indi
  • Swapnil Pradhan Department of Microbiology, Ramnarain Ruia Autonomous College, University of Mumbai, Mumbai, India

Abstract

Melanin is a complex, diverse, negatively charged, hydrophobic, amorphous and high molecular weight hetero-polymeric dark brown photosynthetic pigment synthesized by living organisms including animals, humans, and microorganisms in the course of oxidative polymerization of phenolic compounds. In this study, melanin production was carried out by Pseudomonas sp. which was isolated from soil. After production, Melanin was extracted by acid hydrolysis and characterized based on its solubility in different solvents where it was found to be water-soluble (a unique characteristic of melanin); by TLC and by NMR. After the characterization of melanin, melanin nanoparticles were formed by sonication and then characterized and evaluated for antimicrobial and antioxidative properties. The nanoparticle was found to be of discrete sizes. Melanin nanoparticles showed strong antimicrobial activity against Gram-positive organisms and also higher antioxidative properties than melanin. Melanin Nanoparticles were found to disrupt formed biofilm of Staphylococcus aureus by 67.39%. Thus, Melanin nanoparticles may find their utility as a coating material in medical implants and as packaging material in food products against oxidation and bacterial contamination.

Keywords: Pseudomonas balearica DSM 6083(T), Melanin Nanoparticles, KMnO4, Titanium, Biofilm.

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Author Biography

Swapnil Pradhan , Department of Microbiology, Ramnarain Ruia Autonomous College, University of Mumbai, Mumbai, India

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Additional Files

Published

2020-06-29

How to Cite

Tiwari , A., & Pradhan , S. (2020). Antioxidative, Antibacterial and Antibiofilm Activity of Melanin nanoparticles (MN) . International Journal of Life Sciences, 8(2), 308–316. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/15

Issue

Vol. 8 No. 2 (2020): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2020 Amit Tiwari , Swapnil Pradhan

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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