In Vitro Antagonism of Trichoderma harzianum SDM1 Against Sclerotium rolfsii Associated with Stem Rot Disease of Groundnut (Arachis hypogaea L.)
Keywords:
Groundnut, Sclerotium rolfsii, Dual Culture, Biocontrol, TrichodermaAbstract
Sclerotium rolfsii is one a destructive soil borne fungal pathogen infecting about more than 500 plant species worldwide including groundnut (Arachis hypogea L.). Groundnut is an important oil seed crop in the world. The antagonistic potential of Trichoderma species which has been long known to control various soil-borne fungal pathogens in biological way may be utilized. The faster growth rates with which it competes with fungal pathogen mainly brings upon their antagonistic characteristics. An investigation was carried out in laboratory condition towards biological efficacy of Trichoderma harzianum SDM1 on potato dextrose agar (PDA) medium for the bio-control of soil borne plant pathogen Sclerotium rolfsii in-vitro condition. The dual culture technique was followed in which T. harzianum SDM1showed significant antifungal activities towards the pathogen. In Vitro, T. harzianum SDM1significantly inhibited the mycelial radial growth of S. rolfsii by 64.44±2.2%. The results showed mycelial growth rate for fungal isolate which was determined after 7 days of incubation. Thus, T. harzianum showed encouraging results regarding their biocontrol potential against plant pathogen which may be endorsed to substitute harmful chemical supplements that exists in modern day agricultural practices.
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