Influence of Culture Media on Growth and Sclerotia formation of Rhizoctonia solani
Keywords:
Rhizoctonia solani, Culture media, Colony characteristics, SclerotiaAbstract
Rhizoctonia solani is an important soil-borne fungal pathogen responsible for significant yield losses in many crops. The present study was conducted to evaluate the effect of different culture media on the growth and cultural characteristics of Rhizoctonia solani under in vitro conditions. Six different media, namely Corn Meal Agar, Czapek Dox Agar, Glucose Nitrate Agar, Malt Extract Agar, Nutrient Agar and Potato Dextrose Agar were used for the study. The results revealed significant variation in colony diameter, texture, pigmentation and sclerotia formation across different media. Maximum radial growth was observed on Czapek Dox Agar while minimum growth was recorded on Glucose Nitrate Agar. Early initiation and higher formation of sclerotia were observed in Malt Extract Agar and Czapek Dox Agar, whereas no sclerotia formation was recorded in Glucose Nitrate Agar and Nutrient Agar. The study concludes that culture media significantly influence the growth and morphological characteristics of Rhizoctonia solani.
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