Valorization of Agro-industrial byproducts for high yield Laccase production
Keywords:
Laccase, Brewers spent grain, Peniophora sp,, Solid state fermentationAbstract
Fungal Laccases have higher potential in different industry due to the wide reaction capabilities and broad substrate specificity. Costs and availability of substrates are important factors to consider and therefore the choice of solid substrate plays an important role in determining efficiency in solid fermentation system processes. The objective of this work was to find out substrate for low-cost high profit production of laccase as demand of laccase is high. In this study utilization of Agro industrial biomass waste had applied and various wastes like Tur bagasse, Corn stubs, Soybean bagasse, Groundnut bagasse, Sugarcane bagasse and brewer’s spent grain were used. In present experiment native rotting fungi Peniophora sp collected and screened for laccase production, Result shows that the activity unit of the different types of substrates is significantly different. The laccase activity unit was higher when Brewers spent grain was used as substrates (0.4044µg/ml) which is 45 times greater than the lowest production by sugarcane bagasse as substrates (0.0090 µg/ml). Experimental results confirmed that the best substrate for laccase production is brewers spent grain with nutrient addition. Further statistical optimization needs to be carried to increase the production of laccase enzyme which will help to bio-eliminate the various industrial phenolic waste compounds.
Downloads
References
1. Akermann A, Weiermüller J, Chodorski JN, Nestriepke MJ, Baclig MT, Ulber R (2022) Optimization of bioprocesses with Brewers' spent grain and Cellulomonas uda. Engineering in Life Sciences 22(3-4): 132-151. https://doi.org/10.1002/elsc.202100053
2. Dancker P, Glas K, Gastl M (2025) Potential utilisation methods for brewer's spent grain: A review. International Journal of Food Science and Technology 60(1): vvae022. https://doi.org/10.1093/ijfood/vvae022
3. Lech M, Labus K (2022) The methods of brewers' spent grain treatment towards the recovery of valuable ingredients contained therein and comprehensive management of its residues. Chemical Engineering Research and Design 183: 494-511. https://doi.org/10.1016/j.cherd.2022.05.032
4. Liu K, Xie Z, Huang Y, Suo H, Zhao G, Wang D (2026) Brewers' spent grain reborn via cellulase–xylanase hydrolysis and Lactobacillus acidophilus fermentation and their application in bread. Innovative Food Science & Emerging Technologies 107: 104352. https://doi.org/10.1016/j.ifset.2025.104352
5. Nascimento RP, Junior NA, Pereira Jr N, Bon EPS, Coelho RRR (2009) Brewer's spent grain and corn steep liquor as substrates for cellulolytic enzymes production by Streptomyces malaysiensis. Letters in Applied Microbiology 48(5): 529-535. https://doi.org/10.1111/j.1472-765X.2009.02575.x
6. Nyhan L, Sahin AW, Schmitz HH, Siegel JB, Arendt EK (2023) Brewers' Spent Grain: An Unprecedented Opportunity to Develop Sustainable Plant-Based Nutrition Ingredients Addressing Global Malnutrition Challenges. Journal of Agricultural and Food Chemistry 71(28): 10543-10564. https://doi.org/10.1021/acs.jafc.3c02489
7. Perdani MS, Margaretha G, Sahlan M, Hermansyah H (2020) Solid state fermentation method for production of laccase enzyme with bagasse, cornstalk and rice husk as substrates for adrenaline biosensor. Energy Reports 6: 336-340. https://doi.org/10.1016/j.egyr.2019.08.065
8. Perwez M, Al Asheh S (2025) Valorization of agro-industrial waste through solid-state fermentation: Mini review. Biotechnology Reports 45: e00873. https://doi.org/10.1016/j.btre.2024.e00873
9. Revankar MS, Desai KM, Lele SS (2007) Solid-state fermentation for enhanced production of laccase using indigenously isolated Ganoderma sp. Applied Biochemistry and Biotechnology 143(1): 16-26. https://doi.org/10.1007/s12010-007-0029-0
10. Sharma A, Gupta V, Khan M, Balda S, Gupta N, Capalash N, Sharma P (2017) Flavonoid-rich agro-industrial residues for enhanced bacterial laccase production by submerged and solid-state fermentation. 3 Biotech 7(3): 200. https://doi.org/10.1007/s13205-017-0836-0
11. Sidhu AK, Agrawal SB, Patil SN, Gaikwad VB (2022) Utilizing agro-industrial waste for the production of laccase enzyme by Scytalidium lignicola pesante under solid state fermentation. Agrica 11(2): 117-126. https://doi.org/10.5958/2394-448X.2022.00014.1
12. Solanke K (2025) Screening and Production of Extracellular Fungal Laccase from Peniophora Albobdia by Solid State Fermentation with Agro-industrial Waste. International Journal for Research in Applied Science and Engineering Technology 13(2): 822-827. https://doi.org/10.22214/ijraset.2025.66953
13. Wu J, Li Z, Wang J, Gan H, Wang J, Jin C, Yan G, Yu C, Zhou Y, Wang W (2022) Effects of laccase and cellulase on saccharification of barley malt. Heliyon 8(9): e10744. https://doi.org/10.1016/j.heliyon.2022.e10744
14. Zeko-Pivač A, Tišma M, Žnidaršič-Plazl P, Kulisic B, Sakellaris G, Hao J, Planinić M (2022) The Potential of Brewer's Spent Grain in the Circular Bioeconomy: State of the Art and Future Perspectives. Frontiers in Bioengineering and Biotechnology 10: 870744. https://doi.org/10.3389/fbioe.2022.870744
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/
