Comparative study of cytotoxic effect of Natural and chemical fungicide on onion root tip

Authors

  • Sujata Bondar Department of Botany G.N. Khalsa College of Arts, Commerce and Science (Autonomous), Mayunga (E), Mumbai
  • Manjiri Bhave Department of Botany G.N. Khalsa College of Arts, Commerce and Science (Autonomous), Mayunga (E), Mumbai
  • Shital Jarare Department of Botany G.N. Khalsa College of Arts, Commerce and Science (Autonomous), Mayunga (E), Mumbai

Keywords:

Natural fungicides, chemical fungicides, cytotoxic effect, chromosomal aberrations

Abstract

Fungicides are used to control plant based bacterial and fungal diseases. They can be chemical (synthetic) and natural fungicides, which may show same results but can have different side effects. For current study, we have selected ‘Carbendazim’ as a chemical fungicide, which is widely used to control fungal diseases in crops, vegetables and fruits by inhibiting fungal growth. It is categorized under systematic fungicide which is absorbed by plants and translocated within the tissues, protecting them from inborn and soil born infections. Turmeric, lemon grass and neem are considered under the category of natural fungicides which are extracted in water. Aim of study is to compare and evaluate the possible cytotoxic effect of selected natural and chemical fungicides. Chromosomal aberration such as metaphase with puff chromosome, chromosomal breakage, vagrant chromosomes in anaphase, micronuclei, anaphase bridge, c- mitosis are observed during studies. 

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References

1. Ahmad S, Yasmin R (1992) Effects of methyl parathion and Tri-miltox on the mitosis of Allium cepa. Cytologia 57: 155-160.

2. Aire TA (2005) Short-term effects of carbendazim on the gross and microscopic features of the testes of Japanese quails (Coturnix coturnix japonica). Anatomy and embryology 210(1): 43–49. doi:10.1007/s00429-005-0001-0

3. Al-Najjar NR, Soliman AS (1980) Cytological effects of fungicides. I. mitotic effects of Vitavax-200 and Dithane S-60 on wheat and two related species. Cytologia 45: 163-168.

4. Devi P, Kiranmai V, Padmavathi T (1991) Pesticide induced cytological abnormalities in allium cepa l. J Cytol Genet 26: 13-18.

5. Grover IS, Tyagi PS (1980) Chromosomal aberrations induced by pesticides in meiotic cells of barley. Cytologia 33: 251-261.

6. Grover S, Malhi PK (1988) Genotoxic effects of some organophosphorus pesticides iii. In vivo chromosomal aberration bioassay in root meristems of allium and hordeum. Cytologia 53: 181-191.

7. Kabarity, Mallalah (1980) Cata edulis extract showed a decrease in the rate of cell division in Allium cepa root tip meristem. Cytologia 45: 733-738.

8. Mann (1977) Studied cytological effects of Dithane fungicides which are widely used in the control of blight diseases in fruits, vegetables, onion and other crops were tested for their mutagenic properties on Allium cepa.

9. Mousa M (1982) Mitotic inhibition and chromosomal aberrations induced by some herbicides in root tips of allium cepa. Egypt J Genet Cytol 11: 193-207.

10. Nithyameenakshi S, Jeyaramraja PR, Manian S (2006) Cytotoxicity of the Fungicides Azoxystrobin and Difenoconazole in Root Tips of Allium cepa.

11. Pandita TK (1988) Assessment of the mutagenic potential of a fungicide bavistin using multiple assays. Mutation research/genetic toxicology 204(4): 627-643.

12. Raizada RB, Srivastava MK, Kaushal RA, Singh RP (2001) Azadirachtin, a neem biopesticide: subchronic toxicity assessment in rats. Food Chem Toxicol 39: 477–483.

13. Rank J, Nielsen MH (1997) Allium cepa anaphase-telophase root tip chromosome aberration assay on N-methyl-N-nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfonate. Mut Res 390: 121-127.

14. Rastogi RP, Mehrotra BN (1993) Azadirachta (Meliaceae). In: Rastogi RP (ed) Compendium of Indian Medicinal Plants (1980-1984) vol. 1. Central Drug Research Institute in collaboration with PID publishers, New Delhi, pp. 85-88.

15. Rastogi RP, Mehrotra BN (1995) Azadirachta (Meliaceae). In: Rastogi RP (ed) Compendium of Indian Medicinal Plants (1985-1989), vol. 3. Central Drug Research Institute in collaboration with PID publishers, New Delhi, pp. 87-95.

16. Richmond, Phillips (1975) The effect of benomyl and carbendazim on mitosis in hyphae of Botrytis cinerea Pers. ex Fr. and roots of Allium cepa. Pesticide Biochemistry and Physiology 5(4): 367-379.

17. Sahu RK, Behere BN, Sharma CBSR (1981) Effects of a fungicide, dexoa and its derivatives on root tip systems. The nucleus 24: 60-65.

18. Shehab (1979) Cytological effect of water extract of pulicarianispa on allium cepa. CYTOLOGIA 44(3): 607-613.

19. Shukla Y, Arora A, Taneja P (2002) Anti-mutagenic potential of curcumin on chromosomal aberrations in Wistar rats. MutatRes 515: 197–202.

20. Shukla Y, Arora A, Taneja P (2003) Anti-genotoxic potential of certain dietary constituents. Teratog Carcinog Mutagen 23(s1): 323–335. doi:10.1002/tcm.10059

21. Singh RB (2001) Cytotoxic and mito-depressive effects of pesticides in vicia faba. J Cytol Genet 2: 143-148.

22. Slamenova D, Gabelova A (1980) The effects of sodium azide on mammalian cells cultivated in vitro. MutatRes 71(2): 253–261.

23. Sreejayan N, Rao MN (1996) Free radical scavenging activity of curcuminoids. Arzneimittelforschung 46: 169–171.

24. Sreejayan N, Rao MN (1997) Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 49(1): 105–107.

25. Subramanian M, Sreejayan N, Rao MN, Devasagayam TP, Singh BB (1994) Diminution of singlet oxygen induced DNA damage by curcumin and related antioxidants. MutatRes 311(2): 249–255.

26. Sun YM, Zhang HY, Chen DZ, Liu CB (2002) Theoretical elucidation on the antioxidant mechanism of curcumin: a DFT study. Org Lett 4(17): 2909–2911. doi:10.1021/ol0262789

27. Woo JH, Kim YH, Choi YJ, Kim DG, Lee KS, Bae JH, Min DS, Chang JS, Jeong YJ, et al (2003) Molecular mechanisms of curcumin-induced cytotoxicity: induction of apoptosis through generation of reactive oxygen species, down regulation of Bcl-XL and IAP, the release of cytochrome c and inhibition of Akt. Carcinogenesis 24(7): 1199–1208. doi:10.1093/carcin/bgg082

28. Yildiz M, Evrim SA (2008) Genotoxicity testing of quizalofop-P-ethyl herbicide using the Allium cepa anaphase-telophase chromosome aberration assay. Caryology 61(1): 45–52.

29. Youssef KM, El-Sherbeny MA (2005) Synthesis and antitumor activity of some analogs. Archiv der Pharmazie 338(4): 181–189. doi:10.1002/ardp.200400939

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Published

2026-05-30

How to Cite

Sujata Bondar, Manjiri Bhave, & Shital Jarare. (2026). Comparative study of cytotoxic effect of Natural and chemical fungicide on onion root tip. International Journal of Life Sciences, 14(1A), 333–337. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1638

Issue

Vol. 14 No. 1A (2026): International Journal of Life Sciences

Section

Pest Management & Biological Control

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