Metabolism of indole acetic acid within the root nodules and changes of edaphic factors in the rhizospheric area of Vigna radiata
Keywords:
Vigna radiata, root nodule, indole acetic acid, edaphic factors, symbiosisAbstract
The mature root nodules of Vigna radiata (L.) R. Wilczek (Mung bean), a pulse legume, contained significant amount of indole acetic acid (IAA) and total phenol, which were greater than non-nodulated roots. A remarkable amount of tryptophan pool both in the mature root nodules and the non-nodulated roots were found as the precursor molecule of IAA. Presence of IAA oxidase and peroxidase, the IAA metabolising enzymes, in the mature root nodules and non-nodulated roots indicated the metabolism of IAA in the nodules and roots. Metabolism of phenol by enzymes in both root nodules and roots controlled the IAA production in those tissues. Decomposition of the mature root nodules were increased the percentage of average organic carbon and total nitrogen over control as the root nodules were mixed up with the soil after decomposition. The important physiological implications of the IAA production and the changes of edaphic factors of the rhizospheric area in the light of legume-Rhizobium symbiosis were discussed.
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References
Anderson JPE and Domsch KH (1980) Quantities of plant nutrients in the microbial biomass of selected soils. Soil Sci, 130: 211-216.
Azhrafuzzaman M, Farid AH, Ismail MR, Hoque MA, Islam MZ and Shadidullah SM (2009) Efficiency of plant growth promoting rhizobacteria (PGPR) for the enhancement of rice growth. Afr J Biotechnol, 8:1247-52.
Badenoch-Jones J, Rolfe BG, Letham DS (1983) Phytohormones, Rhizobium mutants and nodulation in legumes. III. Auxin metabolism in effective and ineffective pea root nodules. Plant Physiol, 73: 347-352.
Berrada H and Fikri-Benbrahim K (2014) Taxonomy of the Rhizobia: Current Perspectives. British Microbio Res Journal, 4(6): 616-639.
Bhutani N, Maheshwari R, Negi M and Suneja P (2018) Optimization of IAA production by endophytic Bacillus spp. from Vigna radiata for their potential use as plant growth promoters. Israel J of Plant Sci, 65: 1-2.
Black CA (1965) Methods of Soil Analysis. Vol. 2. American Society of Agronomy.
Bray HG and Thorpe MV (1954) Analysis of phenolic compounds of interest in metabolism. Vol. I. In: Glick, D. (Eds), Methods of Biochemical Analysis. Intersci Publ, New York, 17-52.
Campbell CA, Biederbeck VO, Zentner RP, and Lafond GP (1991) Effect of Crop rotations and cultural practices on soil organic matter, microbial biomass and respiration in a thin black Chernozem. Can J Soil Sci, 71: 363-376.
Christenhusz MJM and Byng JW (2016) The number of known plants species in the world and its annual increase. Phytotaxa, 261 (3): 201–217.
Datta C and Basu PS (1998) Production of Indole Acetic Acid in Root Nodules and Culture by a Rhizobium species from Root Nodules of the Fodder Legume Melilotus alba DESR. Acta Biotechnol, 18(1): 53-62.
Ghosh S and Basu PS (2006) Production and metabolism of indole acetic acid in roots and root nodules of Phaseolus mungo. Microbiol Res, 161: 362-366.
Ghosh S, Sengupta C, Maiti TK and Basu PS (2008) Production of 3-Indolylacetic Acid in Root Nodules and Culture by a Rhizobium Species Isolated from Root Nodules of the Leguminous Pulse Phaseolus mungo. Folia Microbiol, 53(4): 351–355.
Gordon SA and Weber RP (1951) Colorimetric estimation of indole-acetic acid. Plant Physiol, 26: 192-195.
Hassan SSM (1975) Spectrophotometric method for simultaneous determination of tryptophan and tyrosine. Anal Chem, 47: 1429-1432.
Hopkins W G (1999) Introduction to Plant Physiology, 2nd edition. John Wiley and Sons Inc., New York.
Hussain K, Ghaus H, Amin R, Nawaz K, Ahlawat Y, Elhindi KM, Malik A, Bashir S, Iqbal I and Arshad N (2025) Straight Vs. blended application of IAA (Indole Acetic Acid) and Boron (B) for the improvement of crop productivity in maize (Zea mays L.), BMC Plant Biology, 25: 1648.
Jackson ML (1968) Soil chemical Analysis. Prentice-Hall, London.
Jordan DC (1984) Rhizobiaceae. In: Bergey's Manual of Systematic Bacteriology, vol. 1. In: Krieg, N. R., Holt, J. G., (Eds.), Williams and Wilkins Co., Baltimore, U.S.A., 235-240.
Judd WS et al. (2002) Plant Systematics: a phylogenetic approach, Sinauer Associate. Pp. 287-92, ISBN: 978-0-87893-403-4.
Kapur P and Govil SR (2000) Experimental Plant Ecology, CBS Publishers and Distributors (New Delhi).
Kar M and Mishra D (1976) Catalase, peroxidase, and polyphenol oxidase activities during rice leaf senescence. Plant Physiol, 57: 315-319.
Kaushik R, Sharma DK and Joshi HC (2006) Impact on short term changes in soil microbial biomass, carbon and nitrogen dynamics due to irrigation with distillery effluent in rice-wheat cropping symtem. Ind J Microbiol, 46(2): 139-145.
Kefford NP, Brockwell J and Zwar JA (1960) The symbiotic synthesis of auxin by legumes and nodule bacteria and its role in nodule development. Aust J Biol Sci, 13: 456–467.
Kiruthika S and Arunkumar M (2021) A comprehensive study on IAA production by Bradyrhizobium japonicum and Bacillus subtilis and its effect on Vigna radiata plant growth. Indian Journal of Agricultural Research, 55(5): 570-576.
Kokkinakis DM, and Brooks JL (1979) Hydrogen peroxidase mediated oxidation of indole-3-acetic acid by tomato peroxidase and molecular oxygen. Plant Physiol, 64: 220-223.
Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein estimation with folin phenol reagent. J Biol Chem, 193: 265-275.
Mabberley DJ (1997) The plant book, 2nd ed. Cambridge University Press, Cambridge, UK.
Nitsch JP (1955) Free auxin and free tryptophan in strawberry. Plant Physiol, 30: 33-39.
Nutman PS (1977) Study frame works for symbiotic nitrogen fixation. In: Newton W., Postagate J.R., Rodriguez Barrueco C. (Eds.), Recent Developments in Nitrogen Fixation. Academic Press, London, 443–447.
Palraj P, Subraswami V, Zimik Z and Paranthaman S (2025) Indole 3-acetic acid production and growth-promoting effect of Desertifilum sp. PSL17 on Vigna radiata. Frontiers in Industrial Microbiology, 3:1594031.
Pandey PO, Valvi GR and Mahajan JS (2025) Effect of Growth Regulator IAA on Vigna radiata L. (Green Gram). International Journal of Multidisciplinary Research (IJFMR), Vol 7, Issue 3, 1-4.
Panse VG and Sukhatme PV (1985) Statistical Methods for Agricultural Workers, 4th ed. Indian Council of Agricultural Research, New Delhi, 145-156.
Pellock BJ, Cheng HP and Walker GC (2000) Alfalfa root nodule invasion efficiency is dependent on Sinorhizobium meliloti polysaccharides. J Bacteriol, 182: 310-313.
Phillip-Hollingsworth S, Hollingsworth RI and Dazzo FB (1989) Host-range related structural features of the acidic extracellular polysaccharides of Rhizobium trifolii and Rhizobium leguminosarum. J Biol Chem, 264: 1461-1466.
Saleem S, Yasin G, Haq IU, Altaf A, Hussain K and Nawaz K (2021) Indole Acetic Acid (IAA) Mediated Amelioration of Lead (Pb) Stress, Legume Research, Vol 44, Issue 10: 1152-1158
Sinha BK and Basu PS (1981) Indole-3-acetic acid metabolism in root nodules of Pongamia pinnata (L.). Pierre Biochem Physiol Pflanzen, 176: 218-227.
Stafford HA (1974) The metabolism of aromatic compounds. Annu Rev Plant Physiol, 2: 459-486.
Vance CP (1978) Comparative aspects of root and nodule secondary metabolism. Phytochem, 17: 1889-1891.
Verma DPS, Hu CA and Zhang M (1992) Root nodule development: origin, function and regulation of nodulin genes. Physiol Plant, 85: 253–265.
Vogel AI (1961) A Text Book of Quantitative Inorganic Analysis. London, Longman.
Weir BS (2016) The current taxonomy of rhizobia. New Zealand Rhizobia. website. https://www.rhizobia.co.nz/taxonomy/rhizobia.html, Jan, 2016.
Widowati T, Nuriyanah, and Sukiman H (2019) Production of indole acetic acid by Enterobacter cloacea H3 isolated from Mungbean (Vigna radiata) and its potential supporting the growth of soybean seedling. IOP Conf Ser: Earth Environ Sci, 308: 012040.
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