Eco dye & Bio cleanser: Innovating Sustainability with Natural Fabric Dyes and Microbial Decolorizers
Keywords:
Natural fabric dye, Bougainvillea, Balsamina, Clitoria Ternetea, Cestrum Nocturnum, Mirabilis Jalapa, Red Malabar Spinach, bacteria (bacillus species), yeast, fungus (Aspergillus Niger), activated charcoalAbstract
The textile industry is at a turning point with the increasing demand for eco-friendly products. Natural fabric dyes and organic decolorizers are a ray of hope for sustainable clothing. Bougainvillea, Balsamina, Clitoria Ternetea, Cestrum Nocturnum, Mirabilis Jalapa, and Red Malabar Spinach seeds are some of the plant materials used to dye clothes. Cream of tartar is used to enhance dye retention, making clothes more sustainable. Fabrics are immersed directly in extracted pigments for 24 hours after moderation. Top of FormMicrobial decolorizers offer a sustainable solution for removing dyes from fabrics. Bacillus species can produce laccases, peroxidases, and azoreductases that break down synthetic and natural dyes, allowing for efficient decolorization without harsh chemicals. These bacteria can effectively decolorize natural dyes through enzymatic reactions. Microorganisms like Aspergillus Niger and yeast (Saccharomyces cerevisiae) have enzymatic activities that can effectively decolorize natural dyes in fabrics. Activated charcoal is another efficient solution for removing colorants from dye-containing solutions, with great adsorption properties and the ability to be reused. These eco-friendly alternatives offer sustainable solutions for reducing the environmental impact of dyeing processes in the textile industry. We were able to obtain various shades of dyed fabrics by using different plant sources. For instance, Night Jasmine yielded a yellow color, Balsamina flowers produced a yellowish-brown hue, Red Malabar Spinach created a Magenta color, Bougainvillea flowers resulted in a pink shade, Clitoria Ternatea produced a blue color for both cotton and polyester fabrics. At the same time, Curcuma longa (turmeric) and baking soda pigment gave us an orange color and Mirabilis Jalapa was used to dye grey-colored fabric. This paper investigates the decolorization of various dyed fabrics using four different methods: bacteria (bacillus species), yeast, fungus (Aspergillus Niger), and activated charcoal. The results showed that while all four methods were successful in decolorizing the fabrics, Bacteria took 7 days, yeast took 5 days, and fungus and activated charcoal took 9 days to decolorize fabrics. This study provides useful information on removing dyes from textiles.
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References
Grover N, Patani V (2011) High Frequency Plantlet Regeneration Of First Flamed Bush [Woodfordia fruticosa (Linn.) Kurz.] Using Nodal Explant. India Botanical Society 90(3&4). Available at: https://indianbotsoc.org/assets/upload/uploaded/286-291NG%20VP.pdf
Asadulla S, Ramandnag, Rajasekharan (2011) Pharmacognosy of Embelia ribes Burm f. IJRPC 1(1). Available at: https://www.ijrpc.com/files/000060.pdf
Balasubramanian H (2023) Impatiens Balsamina Flower: Uses, benefits and plant care. Available at: https://housing.com/news/impatiens-balsamina/
Singh A, Vyas B (2018) Night Jasmine (Nyctanthes arbortristis). Res J Pharmacognosy and Phytochem 10: 324-330. doi:10.5958/0975-4385.2018.00052.3
Annapoorani G, Sundarraj D (2014) Dyeing of Cotton and Wool Fabric Using Mirabilis Jalapa Flower. IJSR 3(7). Available at: https://www.ijsr.net/archive/v3i7/MDIwMTQxMTUx.pdf
Banat IM, Nigam P, Singh D, Marchant R (1996) Microbial decolorization of textile-dye containing effluents: A review. Bioresource Technology 58(3). doi:10.1016/S0960-8524(96)00113-7
Pearce CI, Lloyd JR, Guthrie JT (2003) The removal of colour from textile wastewater using whole bacterial cells: a review. Available at: https://d1wqtxts1xzle7.cloudfront.net/4641748
Stolz A (2001) Basic and Applied Aspects in the Microbial Degradation of Azo Dyes. Appl Microbiol Biotechnol. doi:10.1007/s002530100686
Telke A, Kadam AA, Gowindwar SP (2014) Bacterial Enzymes and Their Role in Decolorization of Azo Dyes. doi:10.1007/978-3-319-10942-8_7
Iqbal MJ, Ashiq MN (2007) Adsorption of dyes from aqueous solutions on activated charcoal. Journal of Hazardous Materials 139: 57-66. Available at: https://d1wqtxts1xzle7.cloudfront.net/46708081
Dhanalakshmi P, Sathya RI (2022) The Floral Based Natural Dyes and Its Applications. IJCRT. Available at: https://ijcrt.org/papers/IJCRT2207272.pdf
Ding Y, Freeman H (2014) Mordant Dye Application on Cotton Fabric. Available at: https://www.researchgate.net/publication/287955801
Ragab MM, Hassabo AG, Othman HA (2022) An Overview of Natural Dyes Extraction Techniques for Valuable Utilization on Textile Fabrics. J Text Color Polym Sci 19(2): 137-153. Available at: https://jtcps.journals.ekb.eg/article_230202_9cda175adfd34957920286debd244c6c.pdf
Guenthner Johnson P (undated) Using Plants as Natural Dyes. Available at: https://www.ndsu.edu/pubweb/chiwonlee/plsc211/student%20papers/articles04/petra%20Guenthner-Johnson/dyes.html
Kombey P, Rai K, Chaudhary V (2023) Natural Dyes Extracted from Flower Crops: A Review of Recent Advances. European Chemical Bulletin 12(7). Available at: https://www.researchgate.net/publication/372189842
Amir-Al Zumahi SM, Hossain MK, Abser MN, Matin R (2018) Extraction of different natural Dyes from Flower Plants. Available at: https://www.researchgate.net/publication/323416765
Jha CK, Kumar R, Kumar VS, Rajeswari DV (2015) Extraction of natural dye from marigold flower (Tagetes erecta L.) and dyeing of fabric and yarns: A focus on colorimetric analysis and fastness properties. Scholars Research Library 7(1): 185-195. Available at: https://www.scholarsresearchlibrary.com/articles/extraction-of-natural-dye-from-marigold-flower-tageteserectal-and-dyeing-of-fabric-and-yarns-a-focus-on-colorimetric-ana.pdf
Lakshmana Naik R, Sai Shireesha V, Mayuri A, Prakash P, Swetha S (2019) Extraction Of Natural Dyes From The Floral Parts Of Plants And Its Applications In Fabrics. JETIR 6(5). Available at: https://www.jetir.org/papers/JETIR1905352.pdf
Chungkrang L, Bhuyan S, Phukan AR (2021) Natural Dyes: Extraction and Applications. Int J Curr Microbiol App Sci 10(01): 1669-1677. Available at: https://www.ijcmas.com/10-1-2021/Lizamoni%20Chungkrang,%20et%20al.pdf
Botanical Colors (undated) How to Mordant with Aluminum Sulfate. Available at: https://botanicalcolors.com/how-to-mordant-animal-fibers/
Annika (undated) What is a Mordant? (Mordants for Natural Dyes). Available at: https://www.rosemaryandpinesfiberarts.de/what-is-a-mordant/
Rima SAJ, Paul GK, Islam S, Akhtar-E-Ekram M, Zaman S, Saleh MA, Uddin MS (2022) Efficacy of Pseudomonas sp. and Bacillus sp. in textile dye degradation: A combined study on molecular identification, growth optimization, and comparative degradation. Journal of Hazardous Materials Letters 3. Available at: https://www.sciencedirect.com/science/article/pii/S2666911022000211
Barathi S, Aruljothi KN, Karthik C, Padikasan IA (2020) Optimization for enhanced eco-friendly decolorization and detoxification of Reactive Blue 160 textile dye by Bacillus subtilis. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490544
Dhankhar P, Dalal V, Mahto JK, Gurjar BR, Tomar S, Sharma AK, Kumar P (2020) Characterization of dye-decolorizing peroxidase from Bacillus subtilis. Available at: https://pubmed.ncbi.nlm.nih.gov/32971035/
Selvaraj B, Nagarajan AJ, Karthik C, Padikasan IA (2020) Optimization for enhanced ecofriendly decolorization and detoxification of Reactive Blue 160 textile Dye by Bacillus subtilis. Available at: https://www.researchgate.net/figure/Decolourization
Kumar A, Chopra J, Singh SK, Khan A, Singh RN (2015) Biodegradation of azo dyes by Bacillus subtilis. Der Pharmacia Lettre 7(6): 234-238. Available at: https://www.scholarsresearchlibrary.com/articles/biodegradation-of-azo-dyes-by-bacillus-subtilis-ra29.pdf
Ezhilarasu A (2016) Textile industry Dye degrading by bacterial strain Bacillus sp. Int J Adv Res Biol Sci 3(3): 211-226. Available at: https://ijarbs.com/pdfcopy/mar2016/ijarbs27.pdf
Reddy GV, Shah MP (2016) Microbial Decolorization of Various Dyes by a Bacillus subtilis Strain Isolated from an Industrial Effluent Treatment Plant. Available at: https://symbiosisonlinepublishing.com/biotechnology/biotechnology04.php
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