In vitro propagational study of a genetic male sterile line of Capsicum annuum. L

Authors

  • Dr. Ch. G. Gupta Associate Professor, Department of Botany, SR&BGNR Govt. Arts and Science College (A), Khammam, Telangana, India

Keywords:

In Vitro propagation, male sterile line, apical buds, axillary buds, MS Medium, Phytoharmones, IAA, KN, BAP, Shooting, Rooting, Paper bridge

Abstract

In Vitro propagational studies were conducted for a genetic male sterile mutant of Capsicum annuum, which possess marker traits and exhibits very good combining ability with several C. annuum varieties. This genetic male sterile line yielded promising hybrids with high heterotic values. In Vitro propagation attempts were made by taking apical and axillary buds as an explant. The explants were cultured on modified MS medium supplemented with varying concentrations of auxins and cytokinins. Maximum percentage of shooting for both kind of explants was observed in MS medium supplemented with 3 mg BAP + 1mg IAA/ lit. Maximum percentage of rooting was observed in MS medium having 2 mg NAA +0.5 mg KN/ lit.

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References

1. Bahetee GJ, Dhumale DB, Nerker PD (1994) Plant regeneration in tissue cultures of pepper (Capsicum annuum L.) hybrids and varieties. Capsicum and Eggplant Newsletter 13: 65-67.

2. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum 15: 473-497.

3. Murthy NSR, Lakshmi N (1979) Male sterile mutant in Capsicum annuum L. Current Science 48: 312.

4. Sadhana Agarwal, Chandra N, Kothari SL (1988) Shoot tip culture of pepper for micropropagation. Current Science 24: 1347-1349.

5. Sun Z, Wang M (1990) Study on shoot tip meristem culture in Capsicum. Capsicum and Eggplant Newsletter 42: 8-9.

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Published

2026-06-30

How to Cite

Dr. Ch. G. Gupta. (2026). In vitro propagational study of a genetic male sterile line of Capsicum annuum. L. International Journal of Life Sciences, 39–41. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1665

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Section

Research Articles