Biogenesis of Copper Nanoparticles using aqueous extract of Terminalia Chebula fruit and the Study of Antioxidant activity against DPPH.
Abstract
In the present study, phyto-fabricated Copper nanoparticles (CuNPs) were prepared from aqueous fruit extract of Emblica officinalis by a facile, economic, and eco-friendly approach. The facile phyto fabrication of EO-CuNPs was confirmed by UV-visible spectroscopy with characteristic peak around λmax of560-570nm, FTIR spectro-scopic analysis and Zetapotential analysis confirmed that EO-Cu NPs were synthesized by phyto constituents of Emblica officinalis aqueous fruit extract, negative charge (-29.1 mV) reveled the formation stable EO-Cu NPs. The XRD pattern showed that the synthesized EO-Cu NPs were crystalline with an FCC structure. The SEM images showed that EO-Cu NPs were irregularly shaped. The TEM analysis determined that Cu NPs have an average diameter of 11-44 nm and are spherical in shape. The Cu NPs has offered fascinating antioxidant property of EC 50% as 78.73μg/ml, as highest activity as 68.36 at 100μg/ml with dose-dependent activity and photo-catalytic activity against DPPH, with in 26 mins DPPH was completely reduced.
Keywords: Emblica Officinalis (EO), Metabolites, Biosynthesis, Copper Nanoparticles, Antioxidant
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1. Altemimi A, Lakhssassi N, Baharlouei A, Watson DG, Lightfoot DA (2017) Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants 6(4). doi:10.3390/plants6040042
2. Balakrishnan G, Shil S, Vijalakashmi N, Ram M, Rao K, Prabhu K (2019) Green synthesis of copper nanocrystallites using Triphala Churna and their antimicrobial studies 12(9).
3. Baskaran C (2013) Green synthesis of silver nanoparticles using Coleus forskohlii roots extract and its antimicrobial activity against Bacteria and Fungus. Int J Drug Develop Res 5: 1-10.
4. Batoool M, Masood B (2017) Green Synthesis of copper nanoparticles using Solanum lycopersicum (tomato aqueous extract) and study characterization. J Nanosci Nanotechnol Res 1: 1-5.
5. Cheirmadurai K, Biswas S, Murali R, Thanikaivelan P (2014) Green synthesis of copper nanoparticles and conducting nanobiocomposites using plant and animal sources. RSC Advances 4(37): 19507–19511.
6. Cuevas R, et al (2015) Extracellular Biosynthesis of Copper and Copper Oxide Nanoparticles by Stereum hirsutum, a Native White-Rot Fungus from Chilean Forests.
7. Din MI, Arshad F, Hussain Z, Mukhtar M (2017) Green Adeptness in the Synthesis and Stabilization of Copper Nanoparticles: Catalytic, Antibacterial, Cytotoxicity, and Antioxidant Activities. doi:10.1186/s11671-017-2399-8
8. Fatma S, et al (2017) Green synthesis of copper nanoparticle from passiflora foetida leaf extract and its antibacterial activity 10(4).
9. Gopinath K, et al (2016) Green synthesis of silver, gold and silver/gold bimetallic nanoparticles using the Gloriosa superba leaf extract and their antibacterial and antibiofilm activities. Microb Pathog 101: 1–11. doi:10.1016/j.micpath.2016.10.011
10. Gopinath M (2014) Synthesis of copper nanoparticles from Nerium oleander leaf aqueous extract and its antibacterial activity. Int J Curr Microbiol App Sci 3: 814-818.
11. Gunti L, Dass RS, Kalagatur NK (2019) Phytofabrication of Selenium Nanoparticles From Emblica officinalis Fruit Extract and Exploring Its Biopotential Applications: Antioxidant, Antimicrobial, and Biocompatibility 10: 1–17. doi:10.3389/fmicb.2019.00931
12. Gunti L, Dass RS, Kalagatur NK (2019) Phytofabrication of Selenium Nanoparticles From Emblica officinalis Fruit Extract and Exploring Its Biopotential Applications: Antioxidant, Antimicrobial, and Biocompatibility 10: 1–17. doi:10.3389/fmicb.2019.00931
13. Hariprasad S (2016) Green synthesis of copper nanoparticles by Areva lanata leaves extract and their anti-microbial activities. Int J ChemTech Res 9: 14-17.
14. Hulkoti NI, Taranath TC (2014) Biosynthesis of nanoparticles using microbes — A review. Colloids Surfaces B Biointerfaces 121: 474–483. doi:10.1016/j.colsurfb.2014.05.027
15. Ismail M, et al (2019) Green synthesis of zerovalent copper nanoparticles for efficient reduction of toxic azo dyes congo red and methyl orange, pp 135–143.
16. Karthikeyan K, Sang-Jae K (2013) Mater Res Bull 48: 3136.
17. Khan M, et al (2020) Synthesis of au, ag, and au–ag bimetallic nanoparticles using Pulicaria undulata extract and their catalytic activity for the reduction of 4-nitrophenol. Nanomaterials 10(9): 1–14. doi:10.3390/nano10091885
18. Khodaie M, Ghasemi N (2018) Green synthesis and characterization of copper nanoparticles using Eryngium campestre leaf extract 50: 244–250.
19. Kolekar R (2015) Biosynthesis of copper nanoparticles using aqueous extract of Eucalyptus sp. plant leaves. Curr Sci 109: 255-257.
20. Kotval SC, John T, Parmar KA (2018) Green Synthesis of Copper Nanoparticles using Mitragyna parvifolia Plant Bark Extract and Its Antimicrobial Study 4(4): 456–460.
21. Kotval SC, John T, Parmar KA (2018) Green Synthesis of Copper Nanoparticles using Mitragyna parvifolia Plant Bark Extract and Its Antimicrobial Study. J Nanosci Tech 4(4): 456–460. doi:10.30799/jnst.133.18040415
22. Krishna BA, Kumar PN, Prema P (2020) Green synthesis of copper oxide nanoparticles using Cinnamomum malabatrum leaf extract and its antibacterial activity 27: 525–530.
23. Kulkarni N, Muddapur U (2014) Biosynthesis of metal nanoparticles: A review. J Nanotechnol 2014. doi:10.1155/2014/510246
24. Madhuri K, et al (2020) Assessment of the effect of green synthesized silver nanoparticles against aquatic pathogen Aeromonas hydrophila using Artemia nauplii. Indian Journal of Geo Marine Sciences 49(12): 1831-1838.
25. Mohindru JJ, Garg UK (2017) Green synthesis of copper nanoparticles using tea leaf extract. Int J Eng Sci Res Technol 6: 307-311.
26. Murthy HCA, et al (2020) Synthesis of Green Copper Nanoparticles Using Medicinal Plant Hagenia abyssinica (Brace) JF Gmel. Leaf Extract: Antimicrobial Properties.
27. Nasrollahzadeh M, Sajadi SM, Khalaj M (2014) Green synthesis of copper nanoparticles using aqueous extract of the leaves of Euphorbia esula L and their catalytic activity for ligand-free Ullmann-coupling reaction. RSC Advance 4: 47313–47318.
28. Nayantara, Kaur P (2018) Biosynthesis of nanoparticles using eco-friendly factories and their role in plant pathogenicity: a review. Biotechnol Res Innov 2(1): 63–73. doi:10.1016/j.biori.2018.09.003
29. Parthasarathy Srividya, Sanjana Jayacumar, Sudestna Chakraborty et al. Fabrication and Characterization of Copper Nanoparticles by Green Synthesis Approach Using Plectranthus Amboinicus Leaves Extract, 17 August 2020, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-55984/v1].
30. Prateek Khare A, Sharma N, Verma (2014) Synthesis of phenolic precursor-based porous carbon beads in situ dispersed with copper-silver bimetal nanoparticles for antibacterial applications. J Colloid Interface Sci 418: 216–224. doi:10.1016/j.jcis.2013.12.026
31. Raigond P, Raigond B, Kaundal B, Singh B, Joshi A, Dutt S (2017) Effect of zinc nanoparticles on antioxidative system of potato plants. J Env Biol 38: 435.
32. Rajan MS (2004) Nano: The Next Revolution. National Book Trust, India, pp 15-72.
33. Ruparelia JP, Chatterjee AK, Duttagupta SP, Mukherji S (2008) Strain specificity in antimicrobial activity of silver and copper nanoparticles. Acta Biomaterialia 4: 707–716.
34. Saranyaadevi K (2014) Synthesis and characterization of copper nanoparticle using Capparis zeylanica leaf extract. Int J Chem Tech Res 6: 4533-4541.
35. Sharma G, et al (2019) Novel development of nanoparticles to bimetallic nanoparticles and their composites: A review. Journal of King Saud University - Science 31(2): 257-269. doi:10.1016/j.jksus.2017.06.012
36. Shende S, Ingle AP, Gade A, Rai M (2015) Green synthesis of copper nanoparticles by Citrus medica Linn. (Idilimbu) juice and its antimicrobial activity. World J Microbiol Biotechnol 31: 865-873.
37. Subhankari I, Nayak P (2013) Synthesis of copper nanoparticles using Syzygium aromaticum (Cloves) aqueous extract by using green chemistry. World J Nano Sci Technol 2: 14–17.
38. Subhankari I, Nayak PL (2013) Antimicrobial activity of copper nanoparticles synthesised by ginger (Zingiber officinale) extract. World J Nano Sci Technol 2: 10-13.
39. Thirugnanasambandan T, Alagar M (2010) Scholars Research Library.
40. Usha S, Ramappa KT, Hiregoudar S, Vasanthkumar GD (2017) Biosynthesis and Characterization of Copper Nanoparticles from Tulasi (Ocimum sanctum L.) Leaves 6(11): 2219–2228.
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