Evaluation of antioxidant and antimicrobial properties of the muscle extracts of Chrysochir aureus and Otolithes ruberfrom Kakinada Harbour, Andhra Pradesh, India
Keywords:
Antioxidant activity, Antimicrobial activity, Chrysochir aureus, Otolithes ruber, Catla catlaAbstract
Marine fish are important sources of bioactive compounds with potential pharmaceutical and nutraceutical applications. This study evaluated the antioxidant and antimicrobial activities of muscle extracts from Chrysochir aureus and Otolithes ruber. Muscle tissues were extracted using organic solvents and analyzed for antioxidant activity through standard in vitro assays, including free radical scavenging and reducing power tests. Antimicrobial activity was assessed against selected Gram-positive and Gram-negative bacteria, as well as fungal pathogens, using the agar well diffusion method, with minimum inhibitory concentration (MIC) determined where applicable. Both fish muscle extracts exhibited concentration-dependent antioxidant activity, with Chrysochir aureus demonstrating greater free radical scavenging potential than Otolithes ruber. The extracts also showed broad-spectrum antimicrobial activity, producing varying degrees of inhibition against the tested microorganisms. These bioactivities are likely associated with naturally occurring bioactive peptides, proteins, and other antioxidant constituents present in the muscle tissue. The findings indicate that muscle extracts of Chrysochir aureus and Otolithes ruber are promising natural sources of antioxidant and antimicrobial compounds with potential applications in food preservation, pharmaceuticals, and functional foods. Further studies focusing on the isolation, characterization, and in vivo evaluation of these bioactive compounds are needed to confirm their therapeutic potential and elucidate their mechanisms of action.
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