Molecular Identification of Fungal Pathogens Isolated from Bt. Cotton Grown in Saline Soils of Akola and Buldhana Districts, Maharashtra, India

Authors

  • Rupali Deshmukh Department of Microbiology, Shankarlal Khandelwal Art, Science and Commerce College, Akola
  • Dnyansagar Bhokare Department of Microbiology, Shankarlal Khandelwal Art, Science and Commerce College, Akola
  • Yash Sharma Department of Microbiology, Shankarlal Khandelwal Art, Science and Commerce College, Akola

Keywords:

Cotton, Saline Soil, Fungal pathogen, Rhizosphere Sustainable Agriculture

Abstract

Cotton (Gossypium spp.) is one of the world’s most important fiber crops, but its productivity is severely affected by soil salinity and fungal diseases, particularly in the saline–sodic cotton-growing regions of Akola and Buldhana districts in the Vidarbha region of Maharashtra. The present study aims to isolate and identify fungal phytopathogens associated with Bt cotton grown in saline soils. The 32 samples were collected from infected cotton plants from saline-affected fields, and fungal pathogens were isolated on Potato Dextrose Agar (PDA). Three fungal pathogens were identified on the basis of morphological and microscopic examination and molecular characterization. The most frequently recovered species were Aspergillus niger (52%) Fusarium oxysporum (26%), Rhizopus arrhizus (22%). 

 

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References

1. Brown J, Carling DE (2013) Rhizoctonia damping-off and root rot of cotton. The University of Arizona Cooperative Extension. Available at: https://extension.arizona.edu

2. Chen J, Wang Y, Zhang X, Liu L, Ma Z (2023) Molecular mechanisms of pathogenicity and host interactions of Verticillium dahliae. Frontiers in Plant Science 14: 1187563. doi:10.3389/fpls.2023.1187563

3. Davis RM, Colyer PD, Rothrock CS, Kochman JK (2006) Fusarium wilt of cotton: Population diversity and implications for management. Plant Disease 90(6): 692–703. doi:10.1094/PD-90-0692

4. Egamberdieva D, Wirth SJ, Jabborova D, Räsänen LA, Liao H (2022) Coordination between beneficial microorganisms and plants under salt stress. Frontiers in Microbiology 13: 974430. doi:10.3389/fmicb.2022.974430

5. Etesami H, Jeong BR (2021) Contribution of beneficial microorganisms to plant tolerance against environmental stresses. Microbiological Research 242: 126620. doi:10.1016/j.micres.2020.126620

6. Food and Agriculture Organization of the United Nations (2018) International Code of Conduct on Pesticide Management: Guidance on addressing illegal trade of pesticides. FAO. Available at: https://www.fao.org

7. Food and Agriculture Organization of the United Nations (2024) Saline soils and their management. Available at: https://www.fao.org/4/x5871e/x5871e04.htm

8. ICAR–Central Soil Salinity Research Institute (2024) Annual Report 2024. Indian Council of Agricultural Research. Available at: https://cssri.res.in/PSSU/Publications/Annual%20Report/Annual%20Report%202024%20%28Bilingual%29.pdf

9. International Cotton Advisory Committee (2024) World Cotton Statistics. Available at: https://icac.org/publications/statistics

10. Kaur S, Dhillon GS, Brar SK, Vallad GE, Chand R, Chauhan VB (2012) Emerging phytopathogen Macrophomina phaseolina: Biology, economic importance and current diagnostic trends. Critical Reviews in Microbiology 38(2): 136–151. doi:10.3109/1040841X.2011.640977

11. Kirkpatrick TL, Rothrock CS (2001) Compendium of Cotton Diseases (2nd ed.). APS Press.

12. Konde NM, Kanase N, Jadhao SM, Mali DV, Kadu PR (2024) Assessment of water quality in saline tract of Purna Valley in the Vidarbha region. Asian Journal of Soil Science and Plant Nutrition 10(3): 131–143. doi:10.9734/AJSSPN/2024/v10i3325

13. Li N, Han X, Feng D, Yuan D, Huang L (2021) Signaling crosstalk between salicylic acid and reactive oxygen species in plant responses to abiotic stress. Frontiers in Plant Science 12: 661108. doi:10.3389/fpls.2021.661108

14. Press Information Bureau (2025) Ministry of Agriculture and Farmers' Welfare releases Second Advance Estimates of Production of Major Agricultural Crops (Kharif & Rabi) for 2024–25. Government of India. Available at: https://pib.gov.in/pressreleasepage.aspx?PRID=2109884

15. Pitt JI, Hocking AD (2009) Fungi and food spoilage (3rd ed.). Springer. doi:10.1007/978-0-387-92207-2

16. Sharma DK, Minhas PS (2020) Soil salinity and food security in India. Frontiers in Sustainable Food Systems 4: 533781. doi:10.3389/fsufs.2020.533781

17. Sharma R, Patel A, Singh P, Kumar S (2025) Recent advances in the biology and management of Alternaria alternata causing leaf spot diseases in field crops. Journal of Plant Pathology 107(1): 45–62.

18. Zhang Y, Li H, Wang X, Chen L, Xu J (2025) Advances in biological control of Verticillium dahliae: Current status and future prospects. Journal of Fungi 11(2): 156. doi:10.3390/jof11020156

19. Zin NA, Badaluddin NA (2020) Biological functions of Trichoderma spp. For agriculture applications. Annals of Agricultural Sciences 65(2): 168–178. doi:10.1016/j.aoas.2020.09.003

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Published

2026-09-30

How to Cite

Rupali Deshmukh, Dnyansagar Bhokare, & Yash Sharma. (2026). Molecular Identification of Fungal Pathogens Isolated from Bt. Cotton Grown in Saline Soils of Akola and Buldhana Districts, Maharashtra, India. International Journal of Life Sciences, 14(3), 271–278. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1740