Contraceptive effects of aqueous seed extract of Carica papaya (Linn.) on M-isozyme, H-isozyme and M/H ratio in semen of male albino mice (Mus musculus)
Abstract
Objective: To evaluate the effects of Carica papaya seed extract administration on seminal M-isozyme activity, H-isozymes activity and M/H ratio of LDH in mice.
Methods: 60 adult male Swiss albino mice weighing about 25 to 30 gm were randomly divided into two groups. Group 1st (treated group) was fed 0.1 ml of Carica papaya seed extract at a dose of 50 mg/kg body wt. and group II (control group) was fed the same amount of distilled water with similar exposure. After 10 to 50 days of treatment, mice were sacrificed. Semen samples were collected from cauda epididymis of both groups of mice. These samples were filtered, centrifuged, and processed for electrophoresis for the separation of M-isozyme and H-isozymes bands.
Results: Results showed that after 10 to 50 days of papaya seed treatment M-isozyme of LDH activity showed a highly significant (p<0.001) increase in the semen of treated group of mice than the control. However, H-isozyme increased slightly and insignificantly than control. Such an increase in M-isozyme of LDH causes an increase in M/H ratio of LDH in the semen of treated groups of mice than the control.
Conclusions: Significant increase in M-isozyme activity of LDH may impair sperm motility which leads to infertility among papaya seed treated group of mice.
Keywords: Isozymes, epididymis, semen, Infertility,
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Copyright (c) 2020 Soni Kumari, Rajesh Kumar, Vibhuti Narayan Singh
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