Formulation and evaluation of antimicrobial activity of Morus alba sol–gel against periodontal pathogens
Keywords:
Antibacterial activity, Morus alba, periodontal microorganisms, thermoreversible sol–gelAbstract
Antibiotic resistance is a major problem with inadvertent usage. Thus, there is a need to search for new antimicrobial agents of herbal origin to combat antibiotic resistance. One such plant is Morus alba which has a long history of medicinal use in traditional Chinese medicine. To compare the antimicrobial activity of Morus alba sol–gel with chlorhexidine sol–gel against ATCC standard strains of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia. Crude extract of Morus alba leaves was prepared by Soxhlet method by using ethanol as a solvent. Phytochemical screening of the crude extract of M. alba was performed to check the various chemical constituents. M. alba sol–gel and chlorhexidine sol–gel were formulated using Pluronic f127 and Pluronic f108 and compared for their antimicrobial activity. The minimum inhibitory concentration of both the gels was performed using agar well diffusion technique. The minimum inhibitory concentration of M. alba sol–gel and chlorhexidine sol–gel against A. actinomycetemcomitans is 15 and 13 mm, T. forsythia is 7 and 17 mm, and P. gingivalis is 10 and 11 mm, respectively. M. alba possess good antibacterial activity against A. actinomycetemcomitans, P. gingivalis and T. forsythia and thus would be beneficial for the prevention and treatment of periodontal disease.
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