Antibiogram of E coli isolates having heavy metals tolerance

Authors

  • Patil YS Department of Microbiology, Shri D. M. Burungale College of Science and Arts, Shegaon-444203 Dist. Buldana, Maharashtra. India.

Keywords:

Antibiotic resistance, heavy metal resistant bacteria, multiple tolerance.

Abstract

In the present Study total 10 heavy metal resistant E. coli were isolated from sewage of industrial effluents from waste water of Khamgaon M1DC area. Against copper, aluminium, iron, nickel and chromium. All the isolates exhibited high resistance to heavy metals with minimum inhibitory concentration for heavy metals ranging from 50 ug / ml to 500 ug / ml. All isolates showed multiple tolerance to heavy metal and were multi antibiotic resistant, heavy metal tolerance test indicated maximum microbial tolerance of E. coli to cromium (480 ug / ml) and lowest to Copper (60 ug / ml).

Downloads

Download data is not yet available.

References

Aksu Z (1998). Biosorption of heavy metals by microalgae in batch and continuous systems. In: Algae for waste water treatment. eds Y.-S. Wong and N. F. Y. Tam, 99.37-53. Springer, Germany.

Aksu Z and Kutsal T (1990). Acomparative study for biosorption characteristics of heavy metal ions with C. vulgaris. Environ. Technol., 11: 979-987.

Avery SV and Tobin JM (1993). Mechanism of adsorption of hard and soft metal ions to Saccaromyces cerevisiae and influence of hard and soft anions. Appl. Environ. Microbiol, 59: 2851-2856.

Brady D and Duncan JR (1994). Chemical and enzymatic extraction of heavy metal binding polymers from isolated cell walls of Sccharomyces cerevisiae. Biotechnol. Bioeng., 44: 297-302.

Brady D, Stoll AD, Starke L and Duncan JR (1994). Bioaccumulation of metal cations by Saccaromyces cerevisiae. Appl. Microbiol. Biotechnol., 41: 149-154.

Clausen CA (2000). Isolating metal-tolerant bacteria capable of removing copper, chromium, and arsenic from treated wood. Waste Manage Res., 18: 264-268.

Doenmez G and Aksu Z (1999). The effect of copper(II) ions on the growth and bioaccumulation properties of some yeasts. Process Biochem., 35: 135-142.

Dalsgarrd A and L Guardbassi (2002). Occurrence and fate of antibiotics resistant bacteria in sewage. Danish, EPA Environment Project No. 722.

Doenmez G and Aksu Z (2001). Bioaccumulation of copper(II) and Nickel (II)m by the non adapted and adapted growing Candida sp. Wat. Res., 35: 1425-1434.

Filali BK, J Taoufik, Y Zeroual, FAZ Dzairi, M Talbi and M Blaghen (1999). Waste water bacterial isolates resistant to heavy metals and antibiotics.Current Microbiology 41: 151-156.

Huang C, Huang C and Morehart AL (1990). The removal of copper from dilute aqueous solutions by Saccharomyces cerevisiae. Wat. Res., 24: 433-439.

Hussein H, Krull R, Abou-ElEla SI and Hempel DC (1998). Influence of heavy metal ions on the microbial degradation of xenobiotic waste water compounds, AWT98-Advanced wastewater treatment, recycling and reuse, Milano, 14-16 September.

Hussein H, Krull R, Abou-ElEla SI and Hempel DC (2001). Interaction of the different heavy metal ions with immobilised bacterial culture degrading xenobiotic waste water compounds. Second International Water Asociation World Water Conference (2nd IWA) Berlin, 15-19 October.

Koedam N, Wittouck E, Gaballa A, Gillis A, Hfte M and Cornelis P (1994): Detection differentiation of microbial siderophores by isoelectric focusing and chrome azurol S overlay. Biometals, 7: 287- Krieg, N.K. (Ed.) (1984). Bergey`s manual of systematic bacteriology. Baltimore, Hong Kong, London, and sydney, 1: 178-182.

Macaskie L and Dean ACR (1989). Microbial metabolism, desolubilisation and deposition of heavy metals: Metal uptake by immobilised cells and application to the detoxification of liquid wastes. Adv. Biotechnol. Proc., 12: 159-172.

Patterson JW (1977). Wastewater treatment technology. Ann Arbor science Publishers,Ann Arbor, MI, USA.

Rai LC, Gaur JP and Kumar HD (1981). Phycology and heavy metal pollution. Biol. Rev., 56: 99-151.

Silver S (1996). Bacterial resistances to toxic metal ions-a review. Gene 179: 9-19.

Sharma KP, A Frenkel and LD Balkwill (2000). A new Klebsiella planticola strain (cd-1) grows anaerobically at high cadmium concentrations and precipitates cadmium sulphide. Applied and Environmental Microbiology 66: 3083-3087.

Veglio F, Beolchini F and Gasbarro A (1997). Biosorption of toxic heavy metals: an equilibrium study using free cells of Arthrobacter sp. Process Biochem., 32: 99-105.

Volesky B., May H and Holan ZR (1992).Cadmium biosorption by Saccharomyces cerevisiae. Biotechnol. Bioeng., 41: 826-829.

Downloads

Published

2021-03-15

How to Cite

Patil YS. (2021). Antibiogram of E coli isolates having heavy metals tolerance. International Journal of Life Sciences, 53–57. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/398

Issue

Section

Research Articles