Isolation and antimicrobial profiling of pathogenic bacteria from Fish samples
Keywords:
Pathogenic Bacteria, Skin, Gills, Intestine, Buccal cavity, S. typhi, S. aureus, P. aeruginosa, E. coliAbstract
Fish is important nutrient source of protein because it contains more than 60% of protein source. The advantage of fish as a food is that it has easy digestibility and nutritional value. The range of bacterial genera isolated which are related to the aquatic habitat of fish. These include the change in flora during storage. Some disease-causing pathogenic bacteria grow on fish which can survive in freezing temperature and will resume growth when thawing. The bacterial species that are facultative pathogenic for fish and human being and may be isolated from fish without apparent symptoms of the disease. In assessment to isolate and identified these pathogenic bacteria from different variety of fish parts such as Skin, Gills, Intestine, Buccal cavity and study their trends of susceptibility and resistant pattern with various antibiotic, the present work was undertaken. In this study pathogenic bacterium such as S. typhi, S. aureus, P. aeruginosa, E. coli Were isolated. This study demonstrates that fish is potential source of infectious diseases. In antibiotic resistant E. coli and S. typhi was more resistant to gentamycin, and ampicillin. It was also found that S. aureus more resist to gentamycin, ciprofloxacin as compared to amoxicillin, erythromycin, and chloramphenicol.
Downloads
References
Bauer AW, Kirby WMM, Sherris JC, Turck M. (1966). “Antibiotic susceptibility testing by a standardized single disk method”. Amer. J. Clin. Pathol., 45 : 493-6.
Bergey’s manual of Systematic bacteriology, 2nd editition, 1984.
Blanch AR, Hispano C, Bulto P, Balleste E , Lopez J, Gonzale JJ and Vilanova X (2008). Comparison of Vibriospp. population in seawater, in exhibition, in fish intestine and in fish feed. Journal of applied microbiology, 106: 57-65.
Cahill MM. (1990). Bacterial flora of fish; A Review. Microbialecology 19: 21-41
Central Laboratory Standard Institute (CLSI) (2015). “Performance standards for antimicrobial disc susceptibility tests”, Approved standards 10th ed. CLSI document M02-A10, 29 (1).
Clements KD, Angert ER, Montgomery WL, Choat JH. (2014). Intestinal microbiota in fishes: what's known and what's not. Mol Ecol.;23(8):1891-8. doi: 10.1111/mec.12699. Epub 2014 Mar 17. PMID: 24612310.
Dubey RC, Maheshwari P. (2006). “Text book of practical Microbiology”, 2nd edition, S. Chand & company Ltd.
Eze El, Echezona BC, and Uzodinma EC. (2011). Isoaltion and identification of pathogenic bacteria associated with frozen mackerel fish(Scomber scombrus) in a humid tropical environment. African journal of Agriculture Research, 6 (7): 1918-1922.
Gram Lone and Dalgaard (2002). Fish spoilage bacteria – problem and pollution. Current opinion in biotechnology,13: 262-266.
Jain DN, Kamble VA. (2017). Molecular characterization of corneal ulcers causing Staphylococcus aureus. Int J Res Med Sci; 5:1419-24.
Kusuda Richi, and Kawai.Kenji (1998). Bacterial Diseases of Cultured of Culture Marine Fish in Japan. Fishpathology, 33(4):221-227
Masatan SM (2013). Pseudomonas septicemia in labeorohita (HAM).And cyprinuscarpio (LINN.) In Andhra Pradesh –natural occurrence and artificial challenge. International journal of Pharmacy and Pharmaceutical Science, Vol5. ISSN 0975-1491.
Riberio LF, Bardosa MMC, Rezende F de Pinto, Guriz CSL, Maluta RP, Rossi JS, Rossi GAM, Lemon MVF and Amaral (2015).Shiga toxigenic and enteropathogenic E.coli in water and fish from pay to fish pond. AppliedMicrobiology, ISSN 0266-8254.
Sharma Parvati and Shiag RC (2013). Pathogenic Test of bacterial and fungal fish pathogenic in Cirrihinus mrigala infected with EUS Disease. JournalofbiologicalScience, 16 (20): 1204-1207.
Shin JS, Chang S and Kang DH (2004). Application of antimicrobial ice for reduction of fodborne pathogen (E.coli O157:H7, Salmonella typhimurium, Liseria. monocytogenes) on the surface of fish. Journal Applied microbiology, 97: 916-922.
Sichewo Petronillah, Gono Robert, Muzvoudiwa John v , Sizanobouhlez Nayoni (2013). Isolation and identification of pathogenic Bacteria in Edible Fish: A Case Study of Fletcher Dam in Gweru, Zimbabwe. Internationaljournal of scienceand research, 2(9):2319-7064.
Silva, Glavur rogerio Matte Rogerio, Pedro Manuel Leal Germano. Pedro Manuel Leal & Matte Maria Helena (2010) .Occurence of pathogenic microorganisms in fish sold in sao Paulo,Brazil. Journalfood safety, 30:94-110.
Yogoub OS (2009). Isolation of enterobacteriaceae and pseudomonas spp. From raw fish sold in fish market in Khartoumstate. Journalofbacteriologyresearch, 1(7): 85- 88.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Jain Deepika, Hande Roshan
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/