Effect of Nutrient Medium pH on in vitro Shoot Multiplication of Banana
Keywords:
Medium pH, medium autoclaving, agar, multiplication rateAbstract
Nutrient medium provides an ideal condition during in vitro development of plant tissue. The pH of medium is important factor of successful micro propagation besides different medium constituents and other growth components. The experiments were conducted to monitor the changes over time, decline of medium pH and to determine the effect of medium pH on shoot multiplication rate of banana var. Grand Naine. The medium pH was adjusted from pH 5.00 to 6.00 before addition of agar. Results of the experiments concluded that, change of medium pH significantly affects shoot multiplication rate, average weight of shoot clumps and number of roots per shoot. The gelling strength of medium is also affected by changes in pH. The decline in medium pH also observed during storage, but the medium stored in dark condition do not showed change of medium pH. However, prolonged incubation of explants deflects medium pH changes to acidic.
Downloads
References
Chien CC, Rick B and John C. 2015. Effect of environmental and cultural conditions on medium pH and explant growth performance of Douglas-fir (Pseudotsuga menziesii) shoot cultures. F1000 Research. 3: 298.
Dodds JH and Roberts LW. 1990. Aseptic techniques. Experiments in Tissues Culture. 2nd Edition, Cambridge Press, Cambridge. pp. 21-34.
George EF. 1993. The components of culture media, In: George, E. (Ed.), Plant propagation by tissue culture. Exegetics ltd., England, pp. 274-338.
Gomez KA and Gomez AA. 1984. Statistical procedures for agricultural research. John wiley and Sons, New York. pp. 357-427.
Hangarter RP and Stasinopoulos TC. 1991. Effect of Fe-catalyzed photooxidation of EDTA on root growth in plant culture media. Plant Physiol. 96: 843–47.
Mimura T, Shindo C, Kato M, Yokota E, Sakano K, Ashihara H and Shimmen T, 2000. Regulation of cytoplasmic pH under extreme acid conditions in suspension cultured cells of Catharanthus roseus: a possible role of inorganic phosphate. Plant and Cell Physiol. 41: 424-43.
Murashige T and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–97.
Murashige, T. (1974) Plant propagation through tissue culture. Annual review of plant physiology. 25: 135-66.
Owen HR, Wengerd D and Miller AR. 1991. Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal and medium storage method. Plant Cell Rep. 10: 583–86.
Ramage CM and Williams RR: Mineral nutrition and plant morphogenesis. 2002. In Vitro Cell Dev Biol - Plant. 38: 116–24.
Shi X, Yang L, Yan G and Du G. 2017. Medium pH between 5.5 and 7.5 has minimal effects on tissue culture of apple. Hortscience. 52: 475-78.
Singha S, Oberly GH and Townsend EC. 1987. Changes in nutrient composition and pH of the culture medium during in vitro shoot proliferation of crabapple and pear. Plant Cell Tiss Organ Cult. 11: 209–20.
Skirvin RM, Chu MC and Mann ML. 1996. Stability of tissue culture medium pH as a function of autoclaving, time and cultured plant material. Plant Cell Rep. 5: 292–94.
Thorpe T, Stasolla C, and Yeung EC, 2008. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems. George EF et al. (ed), Plant propagation by tissue culture, 3rd edn, the background. (Springer, Dordrecht, The Netherlands). 1: 115–73.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Sherkar Sandip H, Sherkar Harishchandra D
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/
