Aflatoxins and their effects on fish health

Authors

  • Amjad Fatmi Department of Zoology, Govt. College, Dholpur- 328001. Rajasthan, India
  • Durreshahwar Ruby Department of Zoology, B. S. College, Danapur, Patna -800014, Bihar, India
  • Ahmad Masood Department of Botany, H.D Jain. College, Ara - 802301, Bihar, India

Keywords:

Aflatoxins, Aflatoxicosis, immunosuppressive effects, Growth, Survival, Vital organs

Abstract

Aflatoxins are secondary metabolites produced mainly by two molds A. Flavus and A. parasiticus. The molds grow on a variety of imprope-rly stored foods such as Aflatoxin B1 is the most potent of all known aflatoxins and causes severe toxicity known as aflatoxicosis in a variety of animals including human beings who consume contamina-ted foods. Aflatoxicosis is found in fish also and is a matter of great concern for aquaculture industry worldwide. Aflatoxicosis in fish depends upon the species, dose of the toxin as well as the time of exposure. Aflatoxin B1 produces various effect on fish such as retard-ed growth, increased mortality, liver and kidney disfunction, immune-suppression. Long exposure to aflatoxin B1 causes hepatocellular sarcoma and hepatocellular carcinoma in fish.  This review focuses on resources, production, and control measures of aflatoxins to ensure fish safety. The review is informative for research experts in the fields.

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Author Biography

Durreshahwar Ruby, Department of Zoology, B. S. College, Danapur, Patna -800014, Bihar, India

 

 

References

Agriopoulou S, Koliadima A, Karaiskakis G and Kapolos J (2016) Kinetic study of aflatoxins’ degradation in the presence of ozone. Food Control 61, 221–226. doi: 10.1016/j.foodcont.2015.09.013

Ahmad MA and Al-Jewary HA (2012) Effect of Vitamin C on the hepatotoxicity induced by displatin in rats. Raf. J.Sci. 23:-33.

Allocroft R, Carnaghan RBA (1963) Groundnut toxicity. An examination for toxin in human food products from animals fed toxic roundnut meal. Vet. Res., 75: 259.

Almeida IFM, Martins HML, Santos SMO, Freitas MS, Dacosta JMGN, D’Almeida BFM (2011) Mycobiota and aflatoxin B1 in feed for farmedeuropean sea bass (Dicentrarcus labrax). Toxin.3:163-171.

Alpsoy L, Yildrin A, Agar G (2009) The antioxidant effects of vitamin A,C and E on aflatoxin B1 induced oxidative stress in human Lymphocytes. Toxic. Indus. Health., 25:121-127.

Anatar A, Araujo, CMTD, Roch DCC, Ostrensky A, Filho JRE, Ribiero DR, Pimpao CT (2020) Evaluation of growth performance, haematological, biochemical and histopathological parameters of Rhamdia quelen fed with a feed artificially contaminated with aflatoxin B1 Aquaculture reports 17:10036.

Anon A (1993) Monographs on the evaluation of carcinogenic risk to uman: some naturally occurring substance in food items and constituent hetero cyclic aromatic Amines and mycotoxins .No. 56. ARC, Lyon, France, pp. 245-395.

Ayyat MS, Abd Rhman GA, El- Marakby, Mahmuud HK, Hessan AAA (2013) Reduction of aflatoxicity in Nile Tilapia fih. Egyptian J. Nutrition and feeds .16(2) Special issue :469-479.

Bennett JW and Klich M (2003) Mycotoxins. Clin. Microbiol. Rev. 16, 497–516. doi: 10.1128/CMR.16.3.497-516.2003

Blount WP (1961) Turkey “X” Desease. J.Brit. Turkey Fed., 9:52-77.

Buhler DR, Miranda CL, Henderson MC, Yang YH, Lee SJ, Buhler WJL (2000) Effects of 17 beta estradiol and testosterone on hepatic MRNA protein levels and calalytic activities of CYP2M1, CYP2K1 and CYP3A27 in rainbow trout (Oncorhynchus mykiss). Toxico. Appl. Pharmacol., 168: 91-101.

Caguan AG, Tayaban RH, Somga JR, Bartolome RM (2004) Effect of Aflatoxin Contaminated feed in Nile tilapia (Oreochromis niloticus L.). In Proceeding of the 6th International symposium on tilapia in aquaculture (R.B. Remedios, G.C. Imir and K. Fitzsimons. ed), pp. 172 – 178.

Castegnaro M, Hunt DC, Sansone EB, Schuller PL, Sriwardana MG, Telling GM Van, Egmond HP, Walker EA (1980) Laboratory decontamination and destruction of aflatoxin B1, B2, G1, G2 in laboratory wastes. IARC Scientific Publication, 37, Lyon

Centroducati G, Shantacroce MP, Lestingi A, Casalino E, Crescenzo G (2009) Characterization of cellular damage induced by aflatoxin B1 in Sea bream (Sparus aurata L. 1758.) hepatocytes. Ital. J. Anim. Sci., 8: 848-850.

Chang PK, Hua SS (2007) Non aflatoxigenic Aspergillus flavus TX 9-8 completely prevent aflatoxin accumulation by A. flavus isolates of large and small sclerotic morphocytes. Int.J. Food.Microbiol.114:275-279.

Chavez-Sanchez, MC, Martinez PCA, Osorio MI (1994) Pathological effects of feeding young Oreochromis niloticus diets supplemented with different levels of aflatoxin B1. Aquaculture, 127:49-60.

Cheeke PK and Shull LR (1985) Natural Toxicant in feeds and poisonous plants. Abi. Publishing company. ING. West Port. Connecticut.

Cotty PJ, Garcia RJ (2007) Influences of climate on aflatoxin producing fungi and aflatoxin contamination. Int. J. food. Microbiol., 119: 109-15.

Coulombe RA Jr, Bailey GS, Nixon JE (1984) Comparative activation of aflatoxin B1 to mutagens by isolated hepatocytes from rainbow trout (Salmo gaurdneri) and coho salmon (Oncorhynchus Kisutch). Carcinogenesis, 5: 29-33.

Debnath S (2011) Clarias batrachus, the medicinal fish: an excellent c & idate for aquaculture & employment generation. International conference on Asia Agriculture and Animal. IPCBEE.13 2011. IACSIT Press, Singapore.

Deng SX, Tian LX, Liu FG, Jin SJ, Liang GY, Yang HJ, Du ZY, Liu YJ (2010) toxic effect and residue of aflatoxin B1 in tilapia (Oreochromis niloticus × O.aureas) during long term dietary exposure .Aquaculture.307:233-240.

Di Paola JM, Ellis J, Ewin H (1967). Teratogenic response by hamster and mice to aflatoxin. Nature, 215: 638-39.

Diaz GJ, Murcia HW and Cepeda SM (2010) Cytochrome P450 enzymesinvolved in the metabolism of aflatoxin B1 in chickens and quail. Poult. Sci. 89,2461–2469. doi: 10.3382/ps.2010-00864

Diaz DE, Hagler WM, Hopkins BA, Whitlow LW (2002) Aflatoxin binderI: in vitro binding assay for aflatoxin B1 by several potential sequestering agents. Mycopathologia, 156:223-226.

Do JH, choi D (2007) Aflatoxin : Detection, toxicity and biosynthesis. Biotechnol. Bioprocess Eng. 12:585–593.

Doi AM, Patterson PE and Gallagher EP (2002) Variabilityin aflatoxin B1Macro molecular binding and relationship to biotransformation enzyme expression in human prenatal and adult liver. Toxicol. Appl. Pharmacol. 181:48–59

Eaton DL and Gallagher EP (1994) Mechanisms of aflatoxin carcinogenesis. Annu. Rev. Pharmacol. Toxicol. 34:135–172.

El-Boshy ME, El-Ashram AM, El-Ghany, NAA (2008) Effect of dietary beta -1,3Glucagon on immunomodulation on deseased Oreochromis niloticus experimentally infected with aflatoxin B1.

El-Gendy, KS, Aly MN, Mahmoud FH, Kenawy A, El-Sebae AK (2010) The role of vitamin C as antioxidant in protection of oxidative stress induced by imidacloprid food. Chem. Toxic. 48:215-221.

Ellis R, Clement M, Tibbets,A.,Wintree, R.(2000). Reduction of 20µg/kg aflatoxin in trout feed containing clay. Aquaculture 183:179-188.

Enomoto M, Saito M(1972) Carcinogens produced Dutta, H.M., Adikari, S., Singh. N.K., Roy. P.K., Munsi, J.S. (1993). Histopathological changes induced by Malathion in the liver of fresh water cat fish. Heteropneustes fossils (Bloch). Bull. Environ. Contam. Toxicol., 51: 895-900.

Evalyn, W. M., Paul G, M. , Gunner, S, E., James, K.G., Joyce, G. M., Stephen M. , Robert, M. W., Isaac Rumpel ,M., Jan,L.L(2018). Occurrence and Levels of Aflatoxins in Fish Feeds and Their Potential Effects on Fish in Nyeri, Kenya. Toxins 2018, 10,543doi:10.3390/toxins10120543

FAO(2004).world wide regulation for mycotoxins in food and feed in 2003.FAO Food and Nutrition Paper 81.

Ferguson,L.R.,Philpott.M.(2008).Nutritionandmutagenesis.Annu.Rev.Nutr.28:313-329.

Gallagher,E.P.,Kunze,P.L.,Sinclair,P.R.,Stapleton,P.L.,Eaton,D.L(1996).The kinetics of aflatoxin B1 by human cDNA expression and human liver microsomal cytochromes 450 1A2 and 3A4.Toxicol.Appl.Pharmacol.141:595-606.

Galvano, F., Galofaro, V., and Galvano, G. (1996). Occurrence and stability of aflatoxin M1 in milk and milk products. A worldwide review. J. Food Prot. 59, 1079–1090. doi: 10.4315/0362-028X-59.10.1079

Giray, B., Girgin, G., Engin, A. B., Aydın, S., and Sahin, G. (2007). Aflatoxin levels in wheat samples consumed in some regions of Turkey. Food Control 18, 23–29. doi: 10.1016/j.foodcont.2005.08.002

Goldblatt, L. A. (1969). Aflatoxin, Scientific, Background, Control and Implications. Acadmic Press, New York, London.

Guengerich,F.P.,Krausz,W.W.,Johnsos,T.,Shimada,Y.F.,Ueng,H.Y.,Languet,S.(1998).Activation and detoxification of aflatoxin B1.Mutat.Res.402:121-128.

Han,D.,Xie,S.,Zhu,X.,Yang,Y.,Guo,Z.(2009).Growth and hepatopancreas performance of gibel carp fed diet containing low level of aflatoxin B1.Aquaculture Nutrition,16(4):335-342.

Hedayati,M.T.,Pasqualotto,A.C.,Warn,P.A.,Bowyer,P.,andDenning,D.W. (2007). Aspergillus flavus: human pathogen, allergen and mycotoxin producer. Microbiology 153, 1677–1692. doi: 10.1099/mic.0.2007/007641-0

Hendrick,J.D., Bailey,G.S.(1989)Adventitious toxin ,In :Halver JE(ed) Fish nutrition . 2nd edn.Academic Press,San Diego:605-651.

Horn,C.W.,Boleman,LL.,Cofmann,C.G.,Deton,J.H.,LawhornD.B.(1989).Mycotoxin in feed and food producing crops college state Texas.Texas Vet.Med.Diagnostic,The National Dairy Database(1992).

Hseih, D.P., Atkinson, D.N. (1990).Biological reactive intermediates and carcinogenicity. Plenum Press.pp. 525-532. New York.

Hussain, S. Y., Mekkawy, I. A., Z. Z. Moktar, Z.Z., Mubarak, M. (2000). Protective effect of Nigella Sativa seed against aflatoxicosis in Oreochromis niloticus. Mycotoxin Conference, Mycotoxin and environmental Poland. Bydgoszez. 25-27, 109-130.

Hussain,D.,Mateen,A.,Galtin,D.M.(2017).Alleviation of aflatoxin B1 toxicity by calcium bentoniteclay:effects on growth performance,condition indicesand bioaccumulation of AFB1 residue in Nile tilapia(Oreochromis niloticus)Aquaculture.475:8-15.

Hussain,I., Anwar,J.(2008).Astudy on contamination of aflatoxinM1in raw milk in the Punjab Province of Pakistan. Food Control 19, 393–395.

IARC Monographs.(1993). Aflatoxins. Naturally Occurring Aflatoxins(Group1) Aflatoxin M1 (Group 2B). International Agency forResearch on Cancer, Lyon, France. 56, 245.

Jana.H.(2016). Existing problems of fish farming in Indian agriculture.Rashriya Krishi Vol. 11(2). 103-104.

Jantrorotai, I. V., Lovell, R.T.(1990).Subchronic toxicity of diatary aflatoxin B1 to channel cat fish. Journal of Aquatic animal health 2: 248-254.In chickens Mycopathologia, 104: 33-36.

Joner A. (2000) Mycotoxine (http : // www. Ansci Cornell. Edu / courses / as625 / 1999 term / toner / aflatoxin. Html)

Kang, M. S. (1970). Mycotoxin producing potentials of fungi isolated from maiz grain. Ph.D. Thesis, Punjab Agri. University, Ludhiana.

Kenawy,A.M.,ELGenaidy,H.M.,Authman,M.M.N.,AbdelWahab,M.A.(2009).Pathological studies on effects of aflatoxin on Oreochromis niloticus with application of different trials of control. Egypt.J.Com.Path.Clin.Path.,22(1):175-193.

Kheir Eldin, A.A., Motawi, T.M.K. and Sadik, N.A.H. 2008. Effect of some natural antioxidants on aflatoxin B1 induce hepatic toxicity. EXCLI. J. 7: 119131

Kosalec, I., Pepeljnjak, S. (2005). Mycotoxigenicity of clinical and environmental Aspergillus fumigatus and Aspergillus flavus isolates. Acta. Pharm., 55: 365-75.

Lewis,L.,Onsongo,M.,Njapao,H.,SchurzRoger,H.,Luber,G.,Kieszak,S.,Nyamongo,J.,Baker,L.,Dahia,A.MMisore, A.,DeCok,K.,Rubin,C.(2005).Aflatoxin contamination of commercial maizproducts during an outbreak of acute aflatoxicosis in eastern and central Kenya .Environ. Healthperspect.13:1763-1767.

Lillehoj, E.B. (1983). Effects of environmental and cultural factors of aflatoxin in contamination of developing corn carnels.In V.L. Diener, R.L. Asquith and J. W. dickens (ed.) Aflatoxin and A. flavus in corn. Southern coopserv. Bull. 279, Craftsmaster, Opalika, Ala., 112. P

Lovel, R.T.(1972).Protein requirement of cagecultured channel cat fish. Proceedings, Southeast Asian Association of game and fisheries commission.26:357-361

Mahfouz, M.E and Sharif, A.H (2015). A multi parameter investigation into adverse effects of aflatoxin on Oreochromis niloticus health status. J. Bas. Appl. Zool.71: 48-59.

Maryam,A.D.,Parivash,K.,Roshanak,D.G.,Maryam,M.,Ladan,N.,Susan,R. (2018).Inhibitory effect of vitamin C on Aspergillus parasiticus growth and aflatoxin gene expression . Current Medical Mycology.4(3):10-14.

Mclean, M., Dutton, H. F. (1995). Cellular interaction and metabolism of aflatoxin; an update Pharmacol. Therapy, 65: 163-192.

Montesano, R., Hainut, P., Wild, C.P. (1997). Hepatocellular carcinoma. From gene to public health review.J.Nat. Cancer Inst., 89: 1844-1851

Mousa,M.A.A.,EL-Ashram,A.M.M.,Hamed,M.(2008)Effect of neem leaf extract on fresh water fish andzooplankton community 8th international symposium on Tilapia in Aquaculture. proceedings.Cairo,Egypt,12-14 October.2008;pp.307-318.

Nayek ,S.K.,Sawain,p.,Mukherjee,S.C.(2007)Effect of dietary supplement of probiotic and vitamin C on the immune responseof Indian major carp Labeo rohita(Ham).Fish and shellfish immunol.23(4): 892-896.

Newberne, P.M., Rogers, A.E.(1981). Animal toxicity of major environmental toxins. In “Shank R.C. ed. Mycotoxins and N-Nitroso Compounds. Environmental risk Vol.I.CRC Press Inc. Florida, pp. 51-197.

Newberne,P.M., Willium ,H.B.(1969).Acute and chronic effects of aflatoxin on on the liver of domestic and laboratory animals.Cancer Research(29):236-250.

Ngethe, S., Horsberg, T.E., Mitema, E., brigtsen, N. (1993). Species differences in hepatic concentration of orally admenestered 3H – AFB-I between rainbow trout and tilapia. Aquaculture, 114: 355-358.

Nomoto.K.(2005).Prevention of infection by prebiotics.J.Biosciences and Bioengineering,100(2):583-592.

Nunez, J. D.H., Hendricks, J. D., Duimishra, J. R. (1991). Ultra structure of hepato cellular neoplasms in aflatoxin B1 (AFB1) initiated rainbow trout (Oncorhychus mykiss). Toxicol Pathol,. 19:11-21

Nurcan, D., Donmez, H.H., Keskin, E., Kisadere, I. (2012). Effects of aflatoxin on some hematological parameters and protective effectiveness of esterified glucomannan in Marino rams. Sci. World. J.,34: 246-248

Oliviera,C.A.F,.Bavo,F.,Corrasin,C.H.,Jager,A.V,. Reddy,K.R.(2013).Recent trends in microbiological decontamination of aflatoxin in food stuffs in Razzaghi- Abyanah,M.Eds,.Aflatoxin recent advances and future perspectives.Publisher in Tech.Crotia.59-92.

Ong, T. (1975) Aflatoxin mutagenesis. Mutat. Res; 32: 35-58.

Pier, A. C. (1992). Major biological consequences of aflatoxicosis in animalproduction. J. Anim. Sci. 70, 3964–3967. doi: 10.2527/1992.70123964x

Pitt, J. I. (2000). Toxigenic fungi and mycotoxins. Br. Med. Bull., 56 : 184-92

Plakas, S. M., Loveland, P.M., Bailey, G. S., Blazer, V. S., Wilson, G. L. (1991). Tissue deposition and excretion of 14C-labelled Aflatoxin B1 after oral administration in channel cat fish. Food chemistry Toxicology, 29: 805-808.

Raksha,R.K.,Vipin,A.V.,Hariprasad,P.,AnnuAppaiah,K.A.,Venkateswaran.G.Biological detoxification of aflatoxin B1 by Bacillus licheniformis CFR1.Food Control.71:234-241.

Reed, J.D., Casali, O.B.(1987). Hazards to livestock consuming aflatoxin contaminated meal in Africa. In: ICRISAT proceeding of international workshop on aflatoxin contamination in ground nut. 6-9 Oct.1987.

Rodrigues,P.,Venancio,A.,Lima,L.(2012).Aflatoxigenic fungi and aflatoxins in almond.Scie. World J. 9:Article ID 471926,9 pages.

Royes,J.B.,Yanong,R.P.(2002).Mold iin fish feed and aflatoxicosis .Copyright by the University of Florida.University of Agriculture science(UF/IFAS).

Ruby,D.S.,Masood,A.,Fatmi,A.(2014).Effect of aflatoxin contaminated feed on energy reserves of the fish Labeo rohita(Hamilton).Curr.World Environ.9(3):1037-1040.

Sahoo,P.K. and Mukherjee,S.C.(2001).Immunosuppressive effects of aflatoxin B1 in I ndian major carp(Labeo rohita). Comparative immunology, Microbiology and infectious desease.24:143-149.

Sahoo,P.K. and Mukherjee,S.C.(2003)Immunomodulation by dietary vitamin C in healthy and aflatoxin B1 induced immunocompromised rohu(Labeo rohita. Comparative Immunology, Microbiology and infectiousDiseases,26(1):65-76.

Santacroce,M.P.,Conversano,M.C,Cassalino,E.,Lai,O.,Zizzadoro,C.et.al.,(2008). Aflatoxin in aquatic species. Metabolism, toxicity and perspectives. Fish Biology and Fisheries 18:99-130.

Selim, K.M., El-Hofy, H. and Khalil, R.H.( 2014). The efficacy of three mycotoxin adsorbents to alleviate aflatoxin B1 induced toxicity in Oreochromis niloticus. Aquacult. Int. Doi: 10. 1007/s10499-013-9661-6.

Sepahdari. A; Ebrahimzadeh Mosavi. H.A; Sharif pour. I; Khosravi A; Motallebi. A.A; Mohseni. M; kakoolaki. S; pourali, H.R; Hallajian A. (2010). Effect of different diatory level of AFB1 on survival rate and growth factor of Beluga (Huso Huso). Iranian journal of fish. Sci.,9: 141-150.

Severns,D.E.,Clements,M.J.,Lambert,R.J.,andWhite,D.G.(2003). Comparison of Aspergillus earrotandaflatoxincontaminationingrainofhigh- oil andnormaloilcornhybrids. J. FoodProt. 66, 637–643. doi: 10.4315/0362- 028X-66.4.637

Shcherbakova, L., Statsyuk, N., Mikityuk, O., Nazarova, T., and Dzhavakhiya, V. (2015). Aflatoxin B1 degradation by metabolites of Phoma glomerata PG41 isolated from natural substrate colonized by aflatoxigenic Aspergillus flavus. Jundishapur J. Microbiol. 8:e24324. doi: 10.5812/jjm.24324

Soheir ,A.A.M.(2017). Antioxidant activity of ascorbic acid against aflatoxin in contaminated nuts on rats.The J. Of Animal. and Plant sci.27(2):389-397.

Subasinghe, R., Soto, D. and Jia, J. 2009. Global aquaculture and itsrol in sustainable development. Rev. Aquac., 1: 2-9. doi.org/10.1111/j.1753-5131.2008.01002.x.

Teniola, O. D., Addo, P. A., Brost, I. M., Farber, P., Jany, K. D., Alberts, J. F., et al. (2005). Degradation of aflatoxin B1 by cell-free extracts of Rhodococcus erythropolis and Mycobacterium fluoranthenivorans sp. nov. DSM44556T. Int. J. Food Microbiol. 105, 111–117. doi: 10.1016/j.ijfoodmicro.2005.05.004

Tsai,H.W.(1996).Evolution of zebrafish( Brachidanio rerio) as a model for carcinogenrsis.Doctoral dissertation,Origon State University.

Varior,S. And Philip,B(2012)Aflatoxin B1 induced alteration in the stability of lysosomal membrane in Oreochromis mssambicus (Peter 1852).Aquat.Res.43(8):1170-1175.

Verma, R.J.(2004).Aflaroxin causes DNA damage.Int. J. Hum.Genet.4 :231-236.

Verma,R.J and Ravel,P.J.(1989)Morphological alteration in red blood cells after aflatoxin treatment.Interntional Symposium on Agricultural and Biological aspects of aflatoxin related health hazard held at Delhi,22-25 March 1989,pp.21

Verma. R. J., Mehta. D.N. (1998). Occurance of hemolytic anaemia during aflatoxicosis. Indian.J. Environ.Toxicol., 8: 5-7.

Wagachah,J.M., Muthomi,J.W.(2008). Mycotoxin problem in Africa : current status implication to food safety and health and possible management strategies. Int.Food Micro.124: 1-12.

Weidenborner,M.(2001). EncyclopediaofFoodMycotoxins. NewYork,NY: Springer-Verlag.

Wesolek,N.,Roudot,A.C.(2014)Monte Carlo Modeling Aflatoxin B1 Distribution Pistachio Samples:Prerequisite For Sampling Pant Validation .J.Food Tech. Res.1:1-20.

Wu, Q.,Jezkova ,A.,Yuan ,Z.,Pavlikova,L.,Dohnal,V.,and Kuca,K.(2009). Biological degradation of aflatoxins. Drug Metab.Rev. 41, 1–7. doi: 10.1080/ 03602530802563850

Xian,S.,Wang,Y.,Zhao,Q.,Li,M.,Hu,H.,Ma,Y.,Chen,X.,Ni,J.,Zhao,W.,Huang,S.,Wu,L.(2018).Biological degradation of aflatoxin B1 by cells free extract of Bacillus velezensis DY3108 with broad pH stability and excellent thermostability.Toxins.10:330(1-15).

Yu, J. 2012. Current understanding of aflatoxin biosynthesis and future prospective in reducing aflatoxin contamination. Toxins, 4:1024-1057.

Zaki,M.S.,Fawzy,O.F.(2012)Effect of aflatoxin on endocrine status in catfish(Clarias lazera).Life.Sci..,7:591596-596

Zaki; M. S., Fawzy, O. F. And Imam, M. Z. (2011).Reduction of aflatoxin in Clarius lazera cat fish by Ginseng extract and Nigella sativa oil. J American Sci., 7: 591-596.

Zaki; M. S., Fawzy, O. F., Omer,O.,Fauzi,M.,Awad, I.. (2010).Diminution of aflatoxin in Tilapia Zilli fish by dietary supplementation with fix in toxin and Nigella sativa oil.Nature and Science, 8(2): 43-49.

Zycowski,K.E.,Hoffman,A.R.,Ly,H.J.,Pohlenz,C., Buentello, A., Romoster, A.,Gatlin,D.M.A,Philips,T.D.(2013)Effect of aflatoxin on red drum(Scianops ocellatus) and assessment of dietarysupplement of Novasil for prevention of aflatoxicosis.Toxins.5:1555-1573.

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2021-06-30

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Amjad Fatmi, Durreshahwar Ruby, & Ahmad Masood. (2021). Aflatoxins and their effects on fish health . International Journal of Life Sciences, 9(2), 260–268. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/477