Comparative effects of PEG6000 and NaCl simulation osmatic induced solutions stress on morphological seedling traits in wheat plant under lab conditions

Authors

  • Nada A. Asaad Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Nehal G. Abdelaziz Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Shereen A. A. Khalil Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Ali M. Ali Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Hanan E. Mohamed Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Marwa A. Eltony Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Omnia M. Kamal Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Ahmed M. Ahmed Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • E. Ismail Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
  • Dr. Arvind B Chavhan Department of Zoology, Digambarrao Bindu College Bhokar, DIst Nanded
  • Haitham M. A. Elsayed Genetics Department, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.

Keywords:

Wheat Plant, Seed Germination, Seedling Morphology Stage, PEG6000, NaCl, Biploit analysis, Euclidean Distance

Abstract

Development of water and salinity stresses tolerant genotypes through screening is one the most important strategy to overcome these problems. In the present study, seeds of 24 local and exotic genotypes of wheat were allowed to germinate at varying levels of polyethylene glycol (PEG6000) as induced osmotic water stress potential (PEG6000 0 (distilled water as a control treatment), 5%, 10%, 15% and 20%) and NaCl as induced osmotic salinity stress potential (0 mM (distilled water as a control treatment), 50mM, 100 mM, 150 mM and 200 mM) for two weeks on three layers of Whatmann paper in Petri dishes in factorial laboratory experiment with three replicates and complete random design. Data were analyzed with analysis of variance (P<0.01), mean comparisons of LSD at 0.05 and 0.01 levels, biplot analysis and Euclidean distance. When data was found to be significant (P<0.01) the mean comparisons was calculated according to t-test. The results of the study indicated that there was significant decrease on germination parameters (germination percentage) and growth parameters (seedling length, root numbers, seedling fresh and dry weights, tissue water content and vigor index) with subsequent treatments. Biplot analysis classified the tested genotypes in same group as drought and salinity tolerant genotypes. Cluster analysis based on Euclidean distance, classified the genotypes into three groups. These conclusively confirm that the adverse effects of PEG6000 on germination and early seedling growth were due to the osmotic effects rather than the specific ion because of seedling growth was reduced by both stresses, but NaCl usually caused less damage than PEG6000 to wheat seedlings, suggesting that NaCl and PEG6000 acted through different mechanisms. Overall, the genotypes (L (Sids4 X Kasonygiennson-81), Bectora, Canada-462, Benisuaif-6 and CIMMET-113) and (CIMMET-251, CIMMET-270, CIMMET-234, CIMMET-95 and Gimeza-10) showed promising performance against PEG6000 and NaCl simulation levels, respectively in compared to other genotypes due to their superior performance in the study.  

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References

Abdallah Hussein Khalid and Jin Ho Joo. 2018. Plant Growth-Promoting Rhizobacteria Improved Salinity Tolerance of Lactuca sativa and Raphanus sativus. J. Microbiol. Biotechnol. 28(6): 938-945.

Ahmad S. Nariman, Shadia H. S. Kareem, Kamil M. Mustafa and Dastan A. Ahmad. 2107. Early Screening of Some Kurdistan Wheat (Triticum aestivum L.) Cultivars under Drought Stress. Journal of Agricultural Science. 9(2): 88-103.

Ahmed B. Radwa, Aalaaa A. Aboelkassem, Shimaa M. Ali, E. Ismail, Galal A. R. El-Sherbeny and Haitham M. A. Elsayed. 2020. Screening the Responsible Impact of Fourteen Bread Wheat (Triticum aestivum L.) Genotypes against Osmatic Water Stress Mediated through PEG6000 in Terms of Seed Germination and Early Seedling Growth Stage in Search of Promising Drought Tolerant Genotypes under in vitro Condition. Asian Journal of Research in Biosciences. Article no. AJORIB, 172. 1(2): 78-97.

Ali M. Ali, Nada A. Asaad, Marwa A. Eltony, Nehal G. Abdelaziz, Omnia M. Kamal, Ahmed M. Ahmed, Galal A. R. El-Sherbieny and Haitham M. A. Elsayed. 2019. Evaluation Capability of Wheat (Triticum aestivum L.) Genotypes under Salinity (NaCl) Stress as a Systematic Tolerance Assessment at Seed Germination and Early Growth Stage under Laboratory Conditions. Asian Journal of Research in Biosciences. Article no. AJORIB, 171. 1(2): 65-77.

Ali M.N., Yeasmin L., Gantait S. et al. 2014. Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers. Physiol Mol Biol Plants 20:411–423.

Alom Rabiul, Abu Hasan M.d., Rabiul Islam M.d. and Qing-Feng Wang. 2016. Germination Characters and Early Seedling Growth of Wheat (Triticum aestivum L.) Genotypes under Salt Stress Conditions. J. Crop Sci. Biotech. 19 (5): 383- 392.

Ashraf M. and Harris P. J. C. 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166: 3-16.

Babu, S., Sheeba, A., Yogameenakshi, P., Anbumalarmathi, J. and Rangasamy, P. 2007. Effect of salt stress in the selection of salt tolerance hybrids in rice (Oryza sativa L.) under in vitro and in vivo condition. Asian J. Plant Sci. 69:137-142.

Baque Md. Abdullahil, Most. Faijunnahar, Md. Ahsan Habib, Mahmuda Motmainna. 2018. PEG Induced Germination, Seedling Growth and Water Relation Behavior of Wheat Genotypes under Salt Stress Conditions. Universal Journal of Plant Science. 6(3): 21-31.

Bhutto Tofique Ahmed, Abdul Qadir Gola, Muzaffar Hussain Bhutto, Safdar Ali Wahocho, Mahmooda Buriro and Niaz Ahmed Wahocho. 2018. Evaluation of wheat varieties to salt stress (NaCl) for seed germination and early seedling growth under laboratory conditions. Bolan Society for Pure and Applied Biology. http://dx.doi.org/10.19045/bspab.2018.700223.

Bilkis A., Islam M.R., Hafiz M.H.R. and Hasan M.A. 2016. Effect of NaCl induced salinity on some physiological and agronomic traits of wheat. Pak. J. Bot. 48(2): 455-460.

Chachar Zaid, Chachar, N. A., Chachar, Q.I., Mujtaba, S.M., Chachar, G.A. and Sadaruddin Chachar. 2016. Identification of water deficit tolerant wheat genotypes under drought conditions. International Journal of Research – Granthaalayah. 4(2):206-214.

Chaniago Irawati, Auzar Syarif and Putri Riviona. 2107. Sorghum Seedling Drought Response: In Search of Tolerant Genotypes. International Journal on Advanced Science Engineering Information Technology. 7(3): 892-897.

Datta, J.K., Lnag, S., Banerjee, A. and Mondal, N.K. 2009. Impact of salt stress on five varieties of Wheat (Triticum aestivum L.) cultivars under laboratory condition. J. Appl. Sci. Environ. Manage. 13(3): 93-97.

El-Hamamsy, M. A. Sam and Behairy, T. Rehab. 2015. Effect of Salinity Stress on Seedling Vigor and Biochemical Characters of Egyptian Barley Landraces (Hordeum vulgare L.). Middle East Journal of Applied Sciences. 5(3): 786-796.

Farhoudi, R., Modhej, A. and Afrous, A. 2015. Effect of salt stress on Seedlings growth and ions homeostasis of soybean (Glysin max) cultivars. J Scientific Res Dev. 2(5):118-121.

Genc Y., Hu Y.C., Schmidhalter U. 2007. Reassessment of tissue Na+ concentration as a criterion for salinity tolerance in bread wheat. Plant Cell Environ. 30: 1486-1498.

González, L.M., Argental, L., Zaldívar, N. and Ramírez, R. 2005. Effects of simulated drought induced by PEG-6000 on the germination and growth of two wheat varieties. Cultivos Tropicales, 26(4): 49-52.

Hasan M. M., Baque M. A., Habib M. A., Yeasmin M. and Hakim M. A. 2017. Screening of Salt Tolerance Capability of Wheat Genotypes under Salt Stress Condition. Universal Journal of Agricultural Research. 5(4): 235-249.

Hassan Abdul AL-Razak Ali AL-Saady. 2015. Germination and Growth of Wheat Plants (Triticum Aestivum L.) Under Salt Stress. Journal of Pharmaceutical, Chemical and Biological Sciences. 3(3):416-420.

Hellal F.A., El-Shabrawi H.M., Abd El-Hady M., Khatab I.A., El-Sayed Chedly Abdelly S.A.A. 2018. Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. Journal of Genetic Engineering and Biotechnology. 16: 203-212.

Hussein Khalid Abdallah and Jin Ho Joo. 2018. Plant Growth-Promoting Rhizobacteria Improved Salinity Tolerance of Lactuca sativa and Raphanus sativus. J. Microbiol. Biotechnol. 28(6): 938-945.

Ibne Hoque, M.M., Jun, Z., Guoying, W. 2015. Evaluation of salinity tolerance in maize (Zea mays L.) genotypes at seedling stage. J Bio Sci Biotechnology. 4(1): 39-49.

Islam, M.M., Kayesh, E., Zaman, E., Urmi, T.A. and Haque, M.M. 2018. Evaluation of Rice (Oryza sativa L.) Genotypes for Drought Tolerance at Germination and Early Seedling Stage. The Agriculturists. 16(1): 44-54.

Jaleel C. A., Gopi R., Manivannan P. and Panneerselvam R. 2007. Antioxidative potentials as a protective mechanism in Catharan thusroseus (L.) plants under salinity stress. Turkish J. Bot. 31(3): 245–251.

Kandil A.A., Sharief A.E., Abido W.A.E. and Ibrahim M.M.O. 2012. Response of some canola cultivars (Brassica napus L.) to salinity stress and its effect on germination and seedling properties. J. Crop Sci. 3:95-103.

Kausar, F., Hahbaz, M.S, and Ashraf, M. 2013. Protective role of foliar applied nitric oxide in Triticum aestivum under saline stress. Turkish Journal of Botany. 37: 1155-1165.

Kawasaki T., Akiba T., Moritsgu M. 1983. Effects of high concentrations of sodium chloride and polyethylene glycol on the growth and ion absorption in plants. I. Water culture experiments in a green house. Plant Soil, 75: 75-85.

Kayacetin Fatma, Efeoglu Banu and Behrouz Alizadeh. 2018. Effect of NaCl and PEG-Induced Osmotic Stress on Germination and Seedling Growth Properties in Wild Mustard (Sinapis arvensis L.). Anadolu, J. of Aari. 28(1): 62 – 68.

Khakwani Abdul Aziz, Dennett M. D. and Munir M. 2011. Early Growth Response Of Six Wheat Varieties Under Artificial Osmotic Stress Condition. Pak. J. Agri. Sci. 48(2). 119-123.

Murillo-Amador B., Lopez-Aguilar R., Kaya C., Larrinaga-Mayoral J., and Flores H.A. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188: 235-47.

Ouhaddach M., ElYacoubi H., Douaik A. and Rochdi A. 2017. Morpho-Physiological and Biochemical Responses to Salt Stress in Wheat (Triticum aestivum L.) at the Heading Stage. J. Mater. Environ. Sci. 9(6): 1899-1907.

Rana, M.S., Hasan, M.A., Bahadur, M.M. and Islam, M.R. 2017. Effect of Polyethylene Glycol Induced Water Stress on Germination and Seedling Growth of Wheat (Triticum aestivum L.). The Agriculturists, a Scientific Journal of Krishi Foundation. 15(1): 81-91.

Salgotra R. K. et al. 2015. Genetic Diversity and Population Structure of Basmati Rice (Oryza sativa L.) Germplasm Collected from North Western Himalayas Using Trait Linked SSR Markers. PLoS ONE. 10(7): 1–19.

Sandhu N. and Kumar A. 2017. Bridging the Rice Yield Gaps under Drought: QTLs, Genes, and Their Use in Breeding Programs.Agronomy. 7(2): 1–27.

Sayar R., Bchini H., Mosbahi M. and Ezzine M. 2010. Effects of salt and drought stresses on germination, emergence and seedling growth of Durum wheat (Triticum durum Desf.). Journal of Agricultural Research. 5(15): 2008-2016.

Shamaya Nawar Jalal, Yuri Shavrukov, Peter Langridge, Stuart John Roy and Mark Tester. 2017. Genetics of Na+ exclusion and salinity tolerance in Afghani durum wheat landraces. BMC Plant Biology. 17(209): 2-8. DOI 10.1186/s12870-017-1164-6.

Shamim Fakhra, S.M. Saqlan, Habib-Ur-Rehman Athar and Abdul Waheed. 2014. Screening and Selection of Tomato Genotypes/Cultivars for Drought Tolerance Using Multivariate Analysis. Pak. J. Bot., 46(4): 1165-1178.

Sheshaiah, Shankergoud I., Siddhesh R., Nadkarni and Vikas V. Kulakarni. 2017. Screening of Sunflower (Helianthus annuus L) Genotypes for Moisture Stress Tolerance using PEG-6000. Int.J.Curr.Microbiol.App.Sci. 6(5): 848-856.

Slamani Renata Matuszak and Aleksander Brzóstowicz. 2015. Influence of salt stress on growth and frost resistance of three winter cereals. Int. Agrophys. 29: 193-200.

Soare Marin, Paula Iancu, Elena Bonciu, Ovidiu Păniță. 2018. The Effect of Salicylic Acid and Polyetylene Glycol on Wheat Germination. Bulletin USAMV series Agriculture. 75(1): 38-43.

Soare Marin, Paula Iancu, Elena Bonciu, Ovidiu Păniță. 2018. The Effect of Salicylic Acid and Polyetylene Glycol on Wheat Germination. Bulletin USAMV series Agriculture 75(1): 38-43.

Španić Valentina, Maja Ižaković and Tihana Marček. 2017. Wheat Germination And Seedlings Under Peg-Induced Conditons. Agronomski Glasnik 3/2017. ISSN 0002-1954.

Verma H., Borah J. L. and Sarma R. n. 2019. Variability Assessment for Root and Drought tolerance traits and Genetic Diversity Analysis of Rice Germplasm using SSR Markers. Scientificreports. 9:16513 | https://doi.org/10.1038/s41598-019-52884-1

Zhu, Guanglong, Linlin An, Xiurong Jiao, Xubing Chen, Guisheng Zhou, and Neil McLaughlin. 2019. Effects of gibberellic acid on water uptake and germination of sweet sorghum seeds under salinity stress. Chilean Journal of Agricultural Research. 79(3): 415-224. doi:10.4067/S0718-58392019000300415.

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Published

2022-02-21

How to Cite

Nada A. Asaad, Nehal G. Abdelaziz, Shereen A. A. Khalil, Ali M. Ali, Hanan E. Mohamed, Marwa A. Eltony, Omnia M. Kamal, Ahmed M. Ahmed, E. Ismail, Dr. Arvind B Chavhan, & Haitham M. A. Elsayed. (2022). Comparative effects of PEG6000 and NaCl simulation osmatic induced solutions stress on morphological seedling traits in wheat plant under lab conditions. International Journal of Life Sciences, 10(1), 1–20. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/554

Issue

Vol. 10 No. 1 (2022): International Journal of Life Sciences

Section

Research Articles

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Copyright (c) 2022 Nada A. Asaad, Nehal G. Abdelaziz, Shereen A. A. Khalil, Ali M. Ali, Hanan E. Mohamed, Marwa A. Eltony, Omnia M. Kamal, Ahmed M. Ahmed, E. Ismail, Dr. Arvind B Chavhan, Haitham M. A. Elsayed

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