Response surface optimization of some process condition in bioethanol production using fresh water microalgae biomass
Abstract
Background: The increase in the price of fossil fuel, strict government regulations on exhaust emissions and future depletion of worldwide petroleum reserves trigger studies to look for alternative fuels. Bio-ethanol from different kinds of biomass is one way to reduce both consumption of crude oil and environmental pollution. Although extensive efforts have been put in place to evaluate the potential of microalgae as a biofuels feedstock during the past 4–5 decades but there is currently limited information on the state of Microalgae biomass conversion to ethanol, this study aimed at harnessing the potentials of microalgae as third generation biomass for bioethanol production.
Materials and Methods: The hydrolysis of Spirogyra biomass was carried out using dilute acid, amylase and combine acid and enzyme hydrolysis. Fermentation of the algal hydrolysate were done using baker’s yeast (Sacchromyces cereviacea) isolated from sugarcane juice. Some important process conditions (pH, temperature and incubation time) were subjected to optimization using response surface methodology in order to assess their effect in relation to the bioethanol yield. The modeling and statistical analysis were performed using Design expert software, version 6.0.6.
Results: the maximal bioethanol yield of 12 % was obtained with pH of 5.2, at 37.5°C for 152hrs. So also three dimensional contour surface interactions of the parameters shows positive effect of incubation time with less effect of pH and temperature ranges used respectively. Subjecting the product to FT-IR revealed the presence of single carbon bond (2981cm-1), methyl (1417cm-1) and OH group (3331 cm-1) and boiling of 78.73 ± 0.03oC.
Conclusion: Base on the result of this study fresh water Spirogyra spp, could serve as low cost biomass for bioethanol production using combine acid and enzyme hydrolysis at optimized pH, temperature and incubation time.
Keywords: Bioethanol, Spirogyra biomass, Baker’s yeast (Sachromyces Cereviacea), fermentation, hydrolysis.
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Copyright (c) 2020 Mudassir Lawal, Aminu Abdullahi, Abdulazeez M.A, Musa Sale Makeri, Surayya Musa Kalla
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