Anti-Cancerous Effect of Phytochemical Compound (Lectin) on A549 and NCI-H929 Cancer Cell Lines by Cytotoxicity Assay
Keywords:
: Euphorbia tithymaloide,, apoptosis, necrosis, caspase 3, A549 cell line, NCI-H929 cell line, MTT assay, Cytotoxicity, Lectins, Anti-cancerous propertiesAbstract
Cancer is one of the leading reasons of death, worldwide. Cancer is a deadly disease, where the abnormal behaviour of a single cell type is challenging to treat via chemotherapy. It is essential in cancer therapy that the remedy targets only the affected cells, leaving the unaffected cells undisturbed, which is quite difficult, especially in chemotherapy. Anti-cancer drugs reachable in the modern-day market are not target-specific and elicit countless side-effects and issues encountered in the scientific management of a number of forms of cancer, which highlights the pressing need for novel effective and less-toxic therapeutic approaches. Recently, centre of attention has shifted from the usage of lectins to diagnose cancer to actually using lectins to combat cancer.
Evidence is now emerging that lectins are dynamic contributors to tumour cell recognition (surface markers), cell adhesion and localization, signal transduction throughout membranes, mitogenic stimulation, augmentation of host immune defence, cytotoxicity, and apoptosis. In this study we have extracted lectin from plant Euphorbia Tithymaloide (ET). The predominant objective of this project is to check the anti-cancer property of lectins extracted from ET on A549 and NCI-H929 cell lines and a comparative analysis and confirmatory research of its cytotoxicity on these both cells lines.
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