Cytological studies on tissue culture raised plants

Authors

  • Deora Narpat Singh  Associate Professor, SMPBJ Govt. college Sheoganj (Sirohi) 307027, RJ.

Keywords:

stain, colchicine, regenerants, plantlets, aneuoploidy

Abstract

The actively growing root tips of various in vitro raised plantlets i.e. Anogeissus spp., Capparis decidua and M. emarginata were taken and pretreated with colchicine and cold treatment (-4%) for overnight. These pretreated root tips were fixed in 1:3(glacial acetic acid and absolute ethanol). Then these root tips were stained with basic fuchsine (0.5%) prior to make squash.  The stained root tips were squashed in 1% of acetocarmine mordanted with ferric chloride. The squashed root tips were observed under microscope for study of chromosome Most of the in vitro raised plantlets shows chromosome stability but some plantlets shows aneuploidy.

Downloads

Download data is not yet available.

References

Adatia RD and Gavde G (1962) Embryology of the celastraceae. In:Plant Embryology Symposium,CSIR, New Dehli pp. 1-11.

Catlin D, Ochoa A Mac Cormick S and Quiros C (1988) Celery transformation by A tumefaciens. Cytological and genetic analysis of transgenic plants. Plant Cell Report 7:100-103

Evans DA, Sharp WR and Amiratto PV (1983) Hand book of plant cell culture vol. 4 Technique and Application Mcmillon New York, London.

George R and Bingham E (1984). An unstable karyology has also been reported among alfa alfa plants regenerated from callus culture crop. Sci.24:655-658

Gill BS, Bir SS and singhal VK (1979) IOPB chromosome number reports. Taxon 28:391-408.

Nagarajan P and Walton P (1987) A Comparison of samatic chromosomes instability in tissue culture regenerants from medicga media pers Plant cell report 6:10 9-113.

Panikkar AON (1962) chromosome number in capparis decidua, Curr,Sci. 31-32.

Papes D ,Garaj-Vrhovac V, Jelaska S and kovlevska B (1983) chromosome behavior in cultured cell population of higher plants kew chromosome conference 11 George Allen and Unwin London.

Rao SR, Pandey R and Chandel KPS (1993). Genetic stability studies in regenerated plants of Allium tuberosum Rottle ex. Spreng-A cytological approach cytologia 58:31-38.

Shekhawat NS Rathore TS Singh RP Deora NS and Rao SR 1993 Factor affecting in vitro clonal propagation of P. cineraria. Plant growth regu. 12:273-280.

Downloads

Published

2023-04-24

How to Cite

Deora Narpat Singh . (2023). Cytological studies on tissue culture raised plants. International Journal of Life Sciences, 11(1), 109–111. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/740

Issue

Vol. 11 No. 1 (2023): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2023 Author

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/