Hydrocarbon degradation potentials of bacterial species isolated from leachate of Lemna waste dump site Calabar
Keywords:
Hydrocarbons, Dumpsite, Leachate, Degradation, LemnaAbstract
Hydrocarbon degradation potentials of bacteria species isolated from leachate samples of Lemna dumpsite, Calabar, Cross River State were investigated. Five (5) leachate samples were collected from the dumpsite with the use of PVC pipes and were analyzed using standard microbiological techniques. Results of microbial counts from the dumpsite leachates ranged from 10 x 104cfu/g to 2.0 x 105cfu/g. Bacteria isolates, from the leachate samples were subjected to hydrocarbon biodegradability screen test using kerosene, diesel and premium motor spirit (PMS) as substrate for carbon source. Bacteria genera isolated from the analyzed leachate samples were identified as Pseudomonas, Bacillus, Citrobacter, Klebsiella, Aeromonas, Serratia and Shigella. Among these isolates, Pseudomonas (CPS1, CPS2), Bacillus (CPS2, CPS10), Aeromonas (CPS5), Serratia (CPS13) and Corynebacterium (CPS6; CPS16) showed efficient kerosene biodegradability potentials compared to other of their bacteria counterparts. Also Pseudomonas (CPS1, CPS12), Bacillus (CPS2, CPS10), Aeromonas (CPS5) and Serratia (CPS11) were able to biodegrade diesel and premium motor spirit efficiently. However, in comparing hydrocarbon biodegradation potentials among the bacteria isolates from the analyzed leachate samples, Pseudomonas (CPS1, CPS12), Bacillus (CPS2, CPS10), Aeromonas (CPS5) and Serratia (CPS11) were efficient kerosene, diesel and premium motor spirit biodegraders, and this could be useful for bioremediation. This therefore calls for further research by microbiologist and other environmental agencies on the large scale applicability of these isolates for the bioremediation of hydrocarbon polluted environments, as this could serve as a more eco-friendly approach.
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