Evaluation of Fungitoxic efficacy and Physicochemical analysis of Essential Oil of Acorus calamus
Keywords:
Acorus calamus, Rhizome, Clevenger’s apparatus, Essential oil, Fungitoxic efficacy, PhysicochemicalAbstract
The objectives of the study were to determine the fungitoxic efficacy and the physicochemical characteristics of the essential oil extracted from Acorus calamus. 500 g of the rhizome pulp was subjected to hydro-distillation using Clevenger’s apparatus. The fungitoxic efficacy of the essential oil was tested against twenty-five phytopathogenic fungi by using Poisoned food technique. It showed hundred percent fungitoxicity against six fungi out of twenty-five fungi tested. The physicochemical characteristics of essential oil such as colour, specific gravity, specific viscosity, refractive index, acid value, saponification value, ester value, carbonyl percentage, test for phenol and solubility were also determined. These characteristics are used to determine the quality of extracted essential oil. The oil of Acorus calamus was pale yellow and soluble in acetone, ethanol, methanol, benzene and chloroform. The oil exhibited the presence of phenols. The present study indicates that the extraction of essential oil of Acorus calamus has a potential of secondary metabolites which can be exploited as a good source of bioactive substances.
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