Melanoidin degradation in distillery effluent by Bacillus cereus.
Keywords:
Decolourisation, melanoidin, distillery effluentAbstract
Distillery spent wash is a pollution intensive wastewater generated by distilleries. Its dark brown colour is due to recalcitrant melanoidin pigments. The ability of microorganisms to degrade and metabolize a wide variety of compounds has been recognized and exploited in various bio treatment processes. In the present study investigation of the potential of various bacterial cultures isolated from soil at effluent discharge site of distillery was done for decolourization, BOD and COD reduction of effluent. Screening of bacteria having ability to degrade melanoidin pigment and thereby decolorize distillery waste water was followed by estimation of chemical and biochemical oxygen demand of the sample before and after bacterial treatment according to potassium dichromate method and Winkler’s iodometric method. Bacillus cereus demonstrated greater potential for bioremediation as compared to other bacterial isolates. Under optimum conditions Bacillus cereus was able to decolorize the spent wash distillery effluent by 56 % and reduced COD by 63 % after 72 hrs of incubation. Maximum decolourization and COD reduction was found within pH range of 6.5 to 7.0, and temperature range of 25 to 37°C. Microbial decolourization by bacteria is an environment friendly cost-effective alternative to chemical decomposition process before its disposal.
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