Isolation and Identification of Urolytic Bacteria to Produce Biocement
Keywords:
Bio cementation, Urea hydrolysis, Scanning Electron Microscopy, Microorganism, Calcium carbonateAbstract
Application of carbonate precipitation induced by urolytic organisms for improving some properties of cement. Biomineralization is a naturally occurring process in living organism, which induced calcium carbonate precipitation the process of microbially induced calcite precipitation (MICP) is widely used recently in construction engineering in improving compressive strength, durability and self-healing of building materials and cultural heritage. Urease help in mineralization of Calcium carbonate, by hydrolyzing urea present in the environment. It releases carbon dioxide from urease activity, bacteria are able to use urea as sole nitrogen source and produce ammonia, which increase the pH in the proximal environment causing Ca+2 and CO+3 to precipitate as CaCO3. The microbes isolated from the cemented soil sample are tested for Urea hydrolyzing and tolerance capacities. Urease positive bacteria is used to produce Bio cement in-vitro by incubating it for Three week then the precipitated biomass is filtered and air dried, the biomass is checked for its porosity using Scanning Electron Microscope comparing to the ordinary cement. Multiple applications of MICP such as removal of heavy metal and radionuclides, improve the quality of construction materials and sequestration of atmospheric CO2.
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