Screening and detection of Proteinicious Protease Inhibitor from Some Plants from MTR, Maharashtra, India
Keywords:
Protease Inhibitor, X-ray Film, Trypsin, Pepsin, PapainAbstract
Plant protease inhibitors are widely distributed in various plants and majorly reported in seeds. Melghat Tiger Reserved (MTR), Maharashtra region is rich in plant diversity, 714 plant species are reported from the region. The plants used in this study are reported for variety of pharmacological properties and therapeutic purposes. Seeds from 30 different plants from this region were collected and analyzed for the presence of protease inhibitors. Detection of plant protease inhibitors (PI) from the defatted proteinaceous preparation from seed extracts were performed by using X-ray film method. Amongst the selected 18 were detected through X ray film method. Further found to possess the PI capable to inhibit various proteases like trypsin, pepsin and papain. These findings were further confirmed by using quantitative protease inhibition assay by caseinolytic method. The plants of this study reported first time for the presence of trypsin, pepsin and papain inhibitor.
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