Angiogenic Factors Gene Polymorphisms and Pre-disposition to Fibroid Growth in Black Women Attending the Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital, Yaounde
Abstract
Angiogenesis appears to be an important event in the pathophysiology of Uterine Fibroids. Two angiogenic factors, fibroblast growth factor FGF 2 and VEGF, play a central role in the initiation of angiogenesis. We investigated whether the FGF2 754C/G and VEGF 460 C/T polymorphisms are associated with a risk of developing Uterine fibroids in Black Women Attending the Gynaecological Endoscopic Surgery and Human Reproductive Teaching Hospital- CHRACERH, Yaounde. A case-control study was performed on 60 consecutively collected blood samples, spotted on Whartman N° 3-filter paper from which DNA was extracted by the chelex-100 DNA extraction method. Genotyping of the FGF2 and VEGF SNPs was done using the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP). The Chi squared test (X2) was used to establish associations and a p-value of <0.05 was considered statistically significant. There was no difference in genotype distribution of the FGF2 745C/G polymorphism between uterine fibroid cases and controls (P>0.05), but the frequency of the C allele of FGF2 C745G polymorphism in the UF patients was lower than that of control women. The C allele showed to be a risk factor for the development of Uterine fibroids (OR=2.071, 95%CI=0.178-24.150). The Homozygous mutant genotype for the VEGF C460T showed a prevalence of 100% between the UF and control groups. In conclusion, our study demonstrated for the first time that the FGF2 745C/G VEGF 460C/T polymorphisms may be associated with a risk of developing Uterine fibroids in Cameroonian women. Carriers of the GG genotype of FGF2 C745G polymorphism could be protected against the development of Uterine fibroids.
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