Purification, characterization and application of extracellular Tannase by Klebsiellsa pneumoniae isolated from Goat rumen

Authors

  • Selvaraju Gayathri Devi Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore -641014, Tamil Nadu, India.
  • Rahman Fazil Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore -641014, Tamil Nadu, India.
  • Chinnanchettiar Mylsamy Gokulraj Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore -641014, Tamil Nadu, India.
  • Chithra Thangaraju Nandhini Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore -641014, Tamil Nadu, India.
  • Mohanraj Vignesh Department of Biotechnology, Dr. G. R. Damodaran College of Science, Coimbatore -641014, Tamil Nadu, India.

Abstract

Several bacteria were isolated from the partially digested contents of the goat rumen. The bacteria were screened for production of enzyme tannase. One bacterium was found to efficiently utilize tannic acid as a sole carbon source, which was selected as the best tannase producer and utilized for the further study. The bacterium was found as pale yellowish in colour by morphological identification in nutrient agar plates, Upon Gram Staining, it was found to be Gram positive rods. It showed various biochemical characteristics related to the genus Klebsiella. It was further subjected to 16S rRNA sequencing. Blast analysis revealed that the bacteria was closely related to Klebsiella pneumoniae. The crude tannase enzyme was produced from the bacteria and it was purified by Ion exchange and gel filtration chromatography. The specific activity of the crude and purified tannase was found to be 26.1 and 68.2 U/mg respectively. The enzyme had optimal activity at the temperature of 30℃ and pH 5. The addition of calcium, magnesium and manganese were found to increase the enzyme activity. The enzyme activity was influenced by the addition of several detergents. Upon, the use of solid substrates, pomegranate peel was found to be one of the best substrates for high enzyme production. The purified tannase was also found to successfully clarify pomegranate juice in 1 h.

Keywords: Goat rumen, Klebsiella pneumoniae, tannase, pomegranate juice clarification.

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Published

2020-09-30

How to Cite

Selvaraju Gayathri Devi, Rahman Fazil, Chinnanchettiar Mylsamy Gokulraj, Chithra Thangaraju Nandhini, & Mohanraj Vignesh. (2020). Purification, characterization and application of extracellular Tannase by Klebsiellsa pneumoniae isolated from Goat rumen. International Journal of Life Sciences, 8(3), 532–542. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/99

Issue

Vol. 8 No. 3 (2020): International Journal of Life Sciences

Section

Research Articles

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Copyright (c) 2020 Selvaraju Gayathri Devi, Rahman Fazil, Chinnanchettiar Mylsamy Gokulraj, Chithra Thangaraju Nandhini, Mohanraj Vignesh

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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