Screening of antimicrobial Actinomycetes from saline belt of Vidarbha Region
Keywords:
Actinomycetes, Saline belt, Vidarbha region, AntimicrobialAbstract
Total 147 Actinomycetes were isolated from saline belt of Purna river basin which appears in Akola, Amravati and Buldhana district of Vidarbha region. 147 actinomycetes isolates were recovered during primary screening and out of that 87 isolates (59.18 %) showed an antibacterial activity against two test bacteria such as Staphylococcus aureus and Escherichia coli by agar overlay method and 19 isolates were recorded antibacterial against both these test bacteria. These 19 isolates were subjected to secondary screening and found antimicrobial against Staphylococcus aureus (MTCC 7443), Bacillus subtilis (MTCC 441), Escherichia coli (MTCC 443), Pseudomonas aeruginosa (MTCC 424), Aspergillus niger (MTCC 281) and Candida albicans (MTCC 3017) by agar well diffusion method. Nearly 78.94% isolates were active against Staphylococcus aureus followed by 68.42% against Bacillus subtilis, 52.63% against Escherichia coli, 47.36% against Candida albicans, 42.10% against Pseudomonas aeruginosa and 26.31% against Aspergillus niger. Similarly actinomycetes isolate N8 showed activity against all the test microorganisms.
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