Effect of moisture content on the production of protease by Fusarium oxysporum using agroindustrial waste
Keywords:
Agoindustrial waste, Fusarium oxysporum, proteaseAbstract
The effect of moisture content on the production of protease from Fusarium oxysporum was studied using agro industrial waste as substrates such as dal mill waste, oil mill waste, molasses, fruit waste and vegetable garbage under solid state fermentation. Dal mill waste, oil mill waste and vegetable garbage produced maximum protease activity in presence of all the types of moistures (25%, 35%, 45%, 55%, 65%, & 75%) after 96 hrs of incubation. However molasses and fruit waste gave highest protease production in presence of 55 % and 65% moisture content after 7th day of incubation. Among all the substrate dal mill waste and oil mill waste were promising in being utilized faster for the production of protease enzyme.
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Abdus Saboor and Altaff K (1995) Qualitative and Quantitative analysis of Zooplankton population of tropical pond during summer and rainy season. Ecobiol., 7(4):269 – 275. APHA (1998) Standard methods for the examination of water and waste water (20th edition). American Public Health Association, pp.10-161. Chutmanop Jarun, Sinsupha Chuichulcherm, Yusuf Chisti and Penjit Srinophakun (2008) Protease production by Aspergillus oryzea in solid state fermentation using agroindustrial substrates. Chemical Technology, 1018: 1012- 1018. De Azeredo, LAI, Lima MB, de-Coleho RRR and Freire DMG (2006) Thermophilic protease production by Streptomyces sp. 594 in submerged and solid state fermentation using feather meal. J. Appl. Microbiol. 100: 641–647
Germano S, Pandey A, Osaka CA, Rocha SN, Soccol CR (2003) Characterisation and stability of proteases from Penicillium sp. produced by solid-state fermentation. Enzyme Microb Technol., 32:246–251
Hadeer Lazim Hadeer, Houda Mankai, Nedra Slama, Insaf Barkallah and Ferid Limam (2009) Production and optimization of thermophilic alkaline protease in Solid state state fermentation by Streptomyces sp. CN902. J Ind. microbial Biotechnol. vol.36: pp 531-537. Keay L and Wildi BS (1970) Proteases of the genus Bacillus. I. Neutral proteases. Biotechnol. Bioeng., 12: 179–212. doi: 10.1002/bit.260120205
Kota KP and Sridhar P (1999) Solid state cultivation of Streptomyces clavuligerus for cephamycin C production. Process Biochem., 34:325-328. Malathi S and Chakraborty R (1991) Production of Alkaline Protease by a NewAspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. Applied and Environmental Microbiology, 57(3):712-716..
Negi S and Benergee R (2006) Optimization of amylase and protease production from Aspergillus awamori in single bioreactor through EVOP factorial design technique. Food Technol Biotechnol., 44:257-261). Sajeed Ali Syed and Vidhale NN (2013) Protease Production by Fusarium oxysporum in Solid- State Fermentation Using Rice Bran. American journal of microbiological research,1,(3): 45-47.
Sandhya C, Sumantha A, Szakacs G and Pandey, A. (2005) Comparative evaluation of neutral protease production by Aspergillus oryzea in submerged and solid-state Fermentation. Biochemical process., 40: 2689-2694.
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