Effect of moisture content on the production of protease by Fusarium oxysporum using agroindustrial waste

Authors

  • Vidhale NN Department of Microbiology, Shri. Shivaji Science College, Amravati 444604, M.S., India

Keywords:

Agoindustrial waste, Fusarium oxysporum, protease

Abstract

The effect of moisture content on the production of protease from Fusarium oxysporum was studied using agro industrial waste as substrates such as dal mill waste, oil mill waste, molasses, fruit waste and vegetable garbage under solid state fermentation. Dal mill waste, oil mill waste and vegetable garbage produced maximum protease activity in presence of all the types of moistures (25%, 35%, 45%, 55%, 65%, & 75%) after 96 hrs of incubation. However molasses and fruit waste gave highest protease production in presence of 55 % and 65% moisture content after 7th day of incubation. Among all the substrate dal mill waste and oil mill waste were promising in being utilized faster for the production of protease enzyme.

Downloads

Download data is not yet available.

References

Abdus Saboor and Altaff K (1995) Qualitative and Quantitative analysis of Zooplankton population of tropical pond during summer and rainy season. Ecobiol., 7(4):269 – 275. APHA (1998) Standard methods for the examination of water and waste water (20th edition). American Public Health Association, pp.10-161. Chutmanop Jarun, Sinsupha Chuichulcherm, Yusuf Chisti and Penjit Srinophakun (2008) Protease production by Aspergillus oryzea in solid state fermentation using agroindustrial substrates. Chemical Technology, 1018: 1012- 1018. De Azeredo, LAI, Lima MB, de-Coleho RRR and Freire DMG (2006) Thermophilic protease production by Streptomyces sp. 594 in submerged and solid state fermentation using feather meal. J. Appl. Microbiol. 100: 641–647

Germano S, Pandey A, Osaka CA, Rocha SN, Soccol CR (2003) Characterisation and stability of proteases from Penicillium sp. produced by solid-state fermentation. Enzyme Microb Technol., 32:246–251

Hadeer Lazim Hadeer, Houda Mankai, Nedra Slama, Insaf Barkallah and Ferid Limam (2009) Production and optimization of thermophilic alkaline protease in Solid state state fermentation by Streptomyces sp. CN902. J Ind. microbial Biotechnol. vol.36: pp 531-537. Keay L and Wildi BS (1970) Proteases of the genus Bacillus. I. Neutral proteases. Biotechnol. Bioeng., 12: 179–212. doi: 10.1002/bit.260120205

Kota KP and Sridhar P (1999) Solid state cultivation of Streptomyces clavuligerus for cephamycin C production. Process Biochem., 34:325-328. Malathi S and Chakraborty R (1991) Production of Alkaline Protease by a NewAspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. Applied and Environmental Microbiology, 57(3):712-716..

Negi S and Benergee R (2006) Optimization of amylase and protease production from Aspergillus awamori in single bioreactor through EVOP factorial design technique. Food Technol Biotechnol., 44:257-261). Sajeed Ali Syed and Vidhale NN (2013) Protease Production by Fusarium oxysporum in Solid- State Fermentation Using Rice Bran. American journal of microbiological research,1,(3): 45-47.

Sandhya C, Sumantha A, Szakacs G and Pandey, A. (2005) Comparative evaluation of neutral protease production by Aspergillus oryzea in submerged and solid-state Fermentation. Biochemical process., 40: 2689-2694.

Downloads

Published

2015-06-30

How to Cite

Vidhale NN. (2015). Effect of moisture content on the production of protease by Fusarium oxysporum using agroindustrial waste. International Journal of Life Sciences, 3(2), 162–166. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1229

Issue

Vol. 3 No. 2 (2015): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2015 Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/