Estimation of Gallic acid, Rutin and Quercetin in Portulaca quadrifida L. – A potential wild edible plant by HPTLC method

Authors

  • Marathe Vishal R Department of Botany, N.E.S. Science College, Nanded (MS), India
  • Umate Satish K Department of Botany, N.E.S. Science College, Nanded (MS), India

Keywords:

HPTLC, Nutraceuticals, Portulaca quadrifida Wild Edible Plant

Abstract

HPTLC method was developed for the quantitative estimation of gallic acid, rutin and querecetin from methanolic extract of Portulaca quadrifida L. a potential wild edible plant. Precoated silica gel GF254 used as stationary phase and mobile phase for gallic acid was Toulene: Formic acid: Ethyl acetate: Methanol [3:3:8:2, V/V/V/V] and Mobile phase for rutin and querecetin was Ethyl Acetate: Formic acid: Glacial Acetic acid: Water [10:0.5:0.5:1.3, V/V/V/V]. Detection and quantification were performed densitometrically at wavelength λ 254. The Rf values of gallic acid, rutin and quercetin are 0.41, 0.19 and 0.94 respectively. The total peak areas of the standards (gallic acid, rutin and querecetin) and the corresponding peak areas of extract were compared and the gallic acid, rutin and quercetin content were estimated to be 790.9, 2029.7 and 4326.0.

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Published

2016-04-11

How to Cite

Marathe Vishal R, & Umate Satish K. (2016). Estimation of Gallic acid, Rutin and Quercetin in Portulaca quadrifida L. – A potential wild edible plant by HPTLC method. International Journal of Life Sciences, 4(1), 83–88. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1276

Issue

Vol. 4 No. 1 (2016): International Journal of Life Sciences

Section

Research Articles

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