Screening of Acetylcholinesterase inhibitors by Moringa olifera
Keywords:
Alzheimer’s Disease, Acetylcholinesterase, Inhibition, Zebrafish, Plant ExtractsAbstract
Acetylcholinesterase (AChE) is an enzyme involved in the breakdown of the neurotransmitter acetylcholine into acetic acid and choline. Inhibition of Acetylcholinesterase is needed for deceleration of neurodegeneration in Alzheimer’s, Parkinson’s and other neurodegenerative diseases. Plants are known to contain Acetylcholinesterase inhibitors.The methanolic extracts of Moringa olifera leaves were checked for Acetylcholinesterase inhibition on the acetylcholinesterase from brain homogenate of Danio rerio. The activity of the enzyme in-vitro and in-vivo conditions. In our investigations the extract from the plant showed a reduction in the Acetylcholinesterase activity. Under in-vitro conditions and in-vivo conditions the activity of Acetylcholinesterase dropped by 29.26% and 34.26% respectively.
Downloads
References
Anwar F, Latif S, Ashraf M, Gilani AH (2007) Moringa oleifera: a food plant with multiple medicinal uses. Phytother Res., 21: 17-25.
Dennis J Selkoe (2002) Alzheimer’s disease is a Synaptic Failure. Science; 298: 789-791.
DennisJ Selkoe (2001) Alzheimer’s disease: Genes, Proteins and Therapy. Physiological Reviews. : 81
Clark MD, Howe K, Torroja CF, Torrance J etal. (2013) The zebrafish reference genome sequence and its relationship to the human genome. Nature, 496 (7446): 498-503.
Jayanth A and Guruprasad R (2014) Assay of Acetylcholine Esterase Enzyme Activity By Titrimetric Method. International Journal of Pharma and Bio Sciences, 5(4): 643-647
Jayanth A (2014) Muralidhar.S.Talkad. Neuroprotective Activity of Stachytarpheta indica on Rotenone induced Parkinson’s disease in Zebrafish. World Journal of Pharmacy and Pharmaceutical Sciences, 3(7): 940 – 955.
Mark Holden and Cornelius Kelly (2002)) Use of Cholinesterase inhibitors in Dementia. Advances in psychiatric treatments. APT; 8:89-96.
Mehta Mona, Abdu Adem, and Marwan Sabbagh (2012) New Acetylcholinesterase Inhibitors for Alzheimer's Disease, International Journal of Alzheimer's Disease, (2012) Article ID 728983,
8 pages?
Morton JF (1991) The horseradish tree, Moringa pterigosperma (Moringaceae). A boon to arid lands. Econ Bot. 45: 318–333.
Orosco LA, Priyadarshini M, Panula PJ (2013) Oxidative stress and regulation of Pink1 in Zebrafish (Daniorerio). PLoS One. 26; 8(11):e81851. doi: 10.1371/journal.pone.0081851. eCollection 2013. PubMed PMID: 24324558; PubMed Central PMCID: PMC385007.
Rao VA, Devi PU, Kamath R (2001) In vivoradioprotective effect of Moringa oleifera leaves. Indian J Exp Biol. 2001;39: 858–863.
Somali MA, Bajnedi MA, Al-Faimani SS(1984) Chemical composition and characteristics of Moringa peregrina seeds and seed oil. J Am Oil Chem Soc., 61: 85–86.
Rogers SL, Farlow MR, Doody RS, Mohs R, Friedhoff LT and Donepezil(1998) A 24-week, double blind, placebo-controlled trial of donepezil in patients with Alzheimer's disease. Neurology, 50: 136-145.
Leuzinger Walo and Baker AL (1967) Acetylcholinesterase, I. Large-Scale Purification, Homogeneity, and Amino acid Analysis. Proc Natl Acad Sci, U S A. ; l57 (2): 446–451.
Leuzinger W,. Goldberg M, Elsa Cauvin (1069) Molecular properties of acetylcholinesterase. Journal of Molecular Biology.40(2): 217–225
Nair V Priya, Jennifer M Hunter (2004) Anticholinesterases and anticholinergic drugs. Oxford Journals4(5); 164-168.
Sreelatha S, Padma PR (2009) Antioxidant Activity and Total Phenolic Content of Moringa oleifera Leaves in Two Stages of Maturity. Plant Foods Hum Nutr.64: 303-311.
Tsimogiannis DI, Oreopoulou V (2006) The contribution of flavonoid C-ring on the DPPH free radical scavenging efficiency. A kinetic approach for the 3’, 4’- hydroxyl substituted members. Innovative food sci emerg technol., 7: 140-146.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/