β-1,3-Glucanase-Mediated Induction of Disease Resistance in Cicer arietinum (L.) Against Fusarium oxysporum

Authors

  • Aboli Kshirsagar Department of Botany, Arts & Science College, Pulgaon, Dist.- Wardha (MS), India, Affiliated to Rashtrasanta Tukdoji Maharaj Nagpur University, Nagpur- 442302,

Keywords:

β-1,3-glucanase, pathogenesis-related proteins, phytoalexins, plant–pathogen interaction, systemic acquired resistance, Cicer arietinum, Fusarium oxysporum

Abstract

Plant β-1,3-glucanases are pathogenesis-related (PR) enzymes that hydrolyze β-1,3-glucans in fungal cell walls, compromising their structural integrity and inhibiting pathogen growth. These enzymes, along with phytoalexins, constitute key components of plant–pathogen interactions. In this study, the role of β-1,3-glucanase in elicitor-induced defense responses was investigated in resistant (Jaki-9812, Gulak-1), moderately resistant (AKG-46), and susceptible (Chafa-1) cultivars of chickpea (Cicer arietinum L.) against Fusarium oxysporum. Enzyme activity was assayed spectrophotometrically using laminarin as substrate. Results demonstrated rapid induction of β-1,3-glucanase activity in resistant cultivars within 5 hours post-elicitation, peaking at 40–45 hours. Fusarium conidial suspension was the most effective elicitor, followed by reduced glutathione (abiotic) and fungal cell wall elicitor. Tissue-specific expression was highest in cotyledons and roots, and lowest in epicotyls. The study concludes that β-1,3-glucanase activity, along with salicylic acid accumulation, serves as a biochemical marker for disease resistance and plays a vital role in systemic acquired resistance (SAR) in chickpea.

Downloads

Download data is not yet available.

References

Bailey JA, Mansfield JW (1982) Phytoalexins. Blackie, Glasgow.

Barz W, Welle R (1992) Biosynthesis and metabolism of isoflavones and pterocarpan phytoalexins in chickpea, soybean and phytopathogenic fungi. In: Phenolic Metabolism in Plants, Recent Advances in Phytochemistry 26:139–164.

Boller T (1988) Ethylene and the regulation of antifungal hydrolases in plants. Oxford Surveys of Plant Molecular and Cell Biology 5:145–174.

Chester KS (1933) The problem of acquired physiological immunity in plants. Quarterly Review of Biology 8:275–324.

Dixon RA, Harrison MJ (1990) Activation, structure, and organization of genes involved in microbial defense in plants. Advances in Genetics 28:165–234.

Dixon RA, et al. (1995) The phytoalexin response: elicitation, signaling, and control of host gene expression. Biological Reviews 61:239–291.

Grayer RJ, Kokubun T (2001) Plant–fungal interactions: the search for phytoalexins and other antifungal compounds from higher plants. Phytochemistry 56:253–263.

Ingham JL (1976) Induced isoflavonoids from fungus inoculated stems of chickpea (Cicer arietinum L.). Phytochemistry 15:1489–1495.

Ji C, Kuc J (1996) Antifungal activity of cucumber β-1,3 glucanase and chitinase. Physiological and Molecular Plant Pathology 49:257–265.

Kauffmann S, et al. (1987) Elicitor-specific induction of chitinase and β-1,3-glucanase in tobacco. Plant Physiology 85:22–26.

Kesmann H, et al. (1994) Induction of systemic acquired disease resistance in plants by chemicals. Annual Review of Phytopathology 32:439–459.

Linthorst HJM (1991) Pathogenesis-related proteins. Critical Reviews in Plant Sciences 10:123–150. Mansfield JW (1999) Antimicrobial compounds and

resistance. In: Slusarenko AJ, Fraser RSS, van Loon LC (eds) Mechanisms of Resistance to Plant Diseases. Kluwer, pp 325–370.

Mauch F, et al. (1988) Antifungal hydrolases in pea tissue: inhibition of fungal growth by combinations of chitinase and β-1,3-glucanase. Plant Physiology 88:936–942.

Ross AF (1961) Systemic acquired resistance induced by localized virus infections in plants. Virology 14:340-358.

Downloads

Published

2025-09-30

How to Cite

Aboli Kshirsagar. (2025). β-1,3-Glucanase-Mediated Induction of Disease Resistance in Cicer arietinum (L.) Against Fusarium oxysporum . International Journal of Life Sciences, 13(3), 335–339. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/1541

Issue

Vol. 13 No. 3 (2025): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2025 Aboli Kshirsagar

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license unless indicated otherwise in a credit line to the material. If the material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/