Screening of phytochemicals and estimation of secondary metabolites in plant species Euphorbia prostrata L. and Euphorbia parviflora L.
Keywords:
Phytochemicals, Folin-Denis, Alkaloids, Medicinal plants, FlavonoidsAbstract
Phytochemicals, typically secondary metabolites, such as alkaloids, steroids, flavonoids, terpenoids and tannins are the active ingredients that give medicinal plants their therapeutic benefits. Parts of the medicinal plants Euphorbia prostrata and E. parviflora were examined for secondary metabolites. Each secondary metabolite was estimated using a different technique, such as the Folin-Ciocalteu method for phenol, the Folin-Denis method for tannin, and the aluminium chloride colorimetric method for flavonoids. The highest concentrations of phenol, tannin and flavonoids were found in Euphorbia parviflora. In contrast, Euphorbia prostrata had a reduced phenol concentration. Future research on secondary metabolites will require the use of strong analytical separation and detection methods such as GCMS and HR-LCMS.
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