Effect of Sodium bicarbonate on Candida albicans

Authors

  • Varsha Shegokar Kamla Nehru Mahavidyalaya, Nagpur, MS, India
  • Sneha Khadse Project Assistant at CSIR –NEERI, Nagpur, MS, India

Abstract

The purpose of this study was to evaluate the effect of 1% and 10% sodium bicarbonate on the Candida albicans. 5ml of solution from 1% (A) was taken and added to 100ml of Czapek Dox broth and the subcultures were made after every 24 hrs.5ml of solution from 10% (B) was taken and added to 100ml of Czapek Dox broth and subculture were made after every 24 hrs.No sodium bicarbonate was added to 100ml of Czapek Dox broth and subcultures were made after every 24 hrs. The Czapek Dox broth with inoculum (Control flask), the Czapek Dox broth with inoculum with 5ml of solution from 1% (A) and the Czapek Dox broth with inoculum with 5ml of solution from 10% (B) was incubated at 27-300C for 24hrs. After 24 hrs, 0.1ml broth from control flask was added in control plate and was spread over the PDA plate and kept for incubation for 24 hrs at 27-300C. Similarly, 0.1ml of inoculum broth from (A) and 0.1 ml of solution from (B) was added in their respective PDA plates and kept for incubation for 24 hrs at 27-300C. This method was repeated till 168 hrs. By performing the spread plate method, it was found that the growth of candida albicans was inhibited after 168 hrs (after 7 days)

Keywords: Candida albicans, Sodium bicarbonate, Saliva, PDA, Candida hiveg, Czapek Dox Broth.

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Published

2020-06-30

How to Cite

Varsha Shegokar, & Sneha Khadse. (2020). Effect of Sodium bicarbonate on Candida albicans. International Journal of Life Sciences, 8(2), 457–464. Retrieved from https://ijlsci.in/ls/index.php/home/article/view/35

Issue

Vol. 8 No. 2 (2020): International Journal of Life Sciences

Section

Research Articles

License

Copyright (c) 2020 Varsha Shegokar, Sneha Khadse

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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